Báo cáo khoa học: Irregular dimerization of guanylate cyclase-activating protein 1 mutants causes loss of target activation ppt

... (M) 10 -9 10 -8 10 -7 10 -6 10 -5 10 -4 10 -3 10 -2Intensity 10 0 10 5 11 0 11 5 12 0 12 5 13 0CPG-GCAP -1 300 350 400050 10 0 15 0Fluorescence (arbitrary units)Wavelength (nm)[Ca2+] (M) 10 -9 10 -8 10 -7 10 -6 10 -5 10 -4 10 -3 10 -2Intensity6065707580859095WT300 ... Irregular dimerization of guanylate cyclase-activating protein 1 mutants causes loss of target activation Ji-Young Hwang 1, *, Ramona Schlesinger2and Karl-Wilhelm Koch 1 1Institut ... the size of the columns.time (min)0246 810 1 214 16Ca2+monomer (WT)dimerA280CaM-GCAP -1 time (min)0246 810 1 214 16A280EGTACaM-GCAP -1 monomer (WT)dimertime (min)0246 810 1 214 16AAB280EGTACPGmonomer...

Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt

... 10 .55 3. 01 ± 0 .10 1. 00 ± 0.04 0.25 10 . 41 3 .12 ± 0.20 1. 41 ± 0 .13 0.25 9.82 3.05 ± 0 .15 1. 06 ± 0 .18 0.25 10 .07 3.34 ± 0.09 0.96 ± 0.03 1. 00 11 .17 2.50 ± 0 .11 0.78 ± 0.02 0.50 10 .68 3 .18 ± 0.30 1. 29 ... 10 .54 3 .18 ± 0 .15 1. 30 ± 0 .12 0.50 10 .03 3 .11 ± 0 .10 1. 05 ± 0.040.30 10 .38 2.85 ± 0.05 0.87 ± 0.03 0.75 10 .64 3.25 ± 0.20 1. 37 ± 0 .16 1. 00 10 .17 3.05 ± 0 .10 1. 07 ± 0.070.40 10 .50 – – 1. 00 10 .62 ... 0 .16 0.50 9.87 3.08 ± 0 .10 1. 04 ± 0 .12 0.50 10 .54 3. 61 ± 0 .12 0.87 ± 0.04 1. 50 11 .94 2 .10 ± 0.06 0. 61 ± 0.03 0.75 10 .64 3.33 ± 0 .17 1. 33 ± 0 .11 0.75 10 .95 3.83 ± 0.09 0.76 ± 0.03 1. 00 10 .75 3 .15 ...

Tài liệu Báo cáo khoa học: Electrostatic contacts in the activator protein-1 coiled coil enhance stability predominantly by decreasing the unfolding rate docx

... Fig. 4.kf1(M )1 Æs )1 )mu–mt1(calÆmol )1 ÆM )1 ) kf2(s )1 )mI–mt2(calÆmol )1 ÆM )1 ) ku1(s )1 )mf–mt2(calÆmol )1 ÆM )1 ) ku2(s )1 )mI–mt1(calÆmol )1 ÆM )1 )DGkin(kcalÆmol )1 )cJun–FosW ... ER = )1. 45 ( )1. 5)g3)e’4ER = )1. 45 ( )1. 5) ER = )1. 45 ( )1. 5)e2)g’ 1 EK = )1. 15 ( )1. 5) RE = )1. 45 ( )1. 5)e3)g’2RQ = )0.7 ( )1) RE = )1. 45 ( )1. 5)e4)g’3QT = ? (?) RE = )1. 45 ( )1. 5)Total ... ku2(s )1 )mI–mt1(calÆmol )1 ÆM )1 )DGkin(kcalÆmol )1 )cJun–FosW 5.8e6± 1. 3e6 )1. 4 ± 0.2 2.3 ± 0.5 )0.2 ± 0.2 0.046 ± 0. 01 1.0 ± 0 .1 0.92a4.2b??cJun(R)–FosW(E) 7.1e6± 1. 6e6 )1. 9 ± 0.2 4.0 ± 0.7 )1. 0 ± 0 .1 0.00 01 ± 0.00 01 2.5 ± 0.21...

Báo cáo khoa học: Entamoeba histolytica TATA-box binding protein binds to different TATA variants in vitro ppt

... 10 )5 1. 20 · 10 )1 1.48 · 10 )66. 01 · 10 )12 )1. 10 1. 12 · 10 )3)2.86 · 10 )7 1. 20 · 10 )1 3 .16 · 10 )82.76 · 10 )15 2 4.37 1. 56 · 10 )2)2.77 · 10 )58.24 · 10 )1 1.25 · 10 )56. 31 · 10 )11 )2 .19 ... · 10 )52.44 · 10 )1 3. 71 · 10 )6 1. 87 · 10 )11 )1. 86 2.23 · 10 )3)6. 71 · 10 )72.44 · 10 )1 9 .13 · 10 )8 1. 13 · 10 )14 6I 4. 61 7.62 · 10 )3 )1. 24 · 10 )56.54 · 10 )29.94 · 10 )75. 01 · 10 )12 )1. 17 ... 10 )11 )2 .19 2.74 · 10 )5)8.56 · 10 )78.24 · 10 )1 3.87 · 10 )76.03 · 10 )14 3 5.04 7. 91 · 10 )3 )1. 16 · 10 )5 1. 68 · 10 )1 2.55 · 10 )6 1. 29 · 10 )11 )1. 34 1. 24 · 10 )3)2.87 · 10 )7 1. 68 · 10 )1 6.29...

Báo cáo khoa học: "Why Initialization Matters for IBM Model 1: Multiple Optima and Non-Strict Convexity" pptx

... stdev unif 1 100 10 0 10 0 - 2.9K -4.9K8 83.6 89.0 10 0 33.3 2.3K -2.3K32 77.8 81. 8 10 0 17 .9 874 74.4 12 8 67.8 73.3 99.7 17 .7 270 2721K 52.6 64 .1 99.8 24.0 220 2 81 10K 30.3 47.33 99.9 24.4 15 0 300Table ... 28.89 10 0K-unif-5 28.98 - - 12 .68 - - 10 0K-rand-5 28.63 28.99 30 .13 12 .25 12 .62 12 .89 10 0K-unif-∞ 28 .18 - - 16 .84 - - 10 0K-rand-∞ 27.95 28.22 30 .13 16 .66 16 .78 16 .85Table 2: AER results for Model 1 and HMM using ... Take x = [x 1 , x2] = [ .1, .2],y = [y 1 , y2] = [.2, .1] and θ = .5. We havez = θx + (1 − θ)y = [z 1 , z2] = [ .15 , .15 ]. Also− log (1 T(θx + (1 − θ)y)) = − log(z 1 + z2) =− log(.3)....

Tài liệu Báo cáo khoa học: Secondary substrate binding strongly affects activity and binding afﬁnity of Bacillus subtilis and Aspergillus niger GH11 xylanases docx

... 0.08N141Q 1. 01 ± 0 .14 N54W-N141Q 1. 00 ± 0 .11 XANWild-type 1. 00 ± 0.09D16A 1. 03 ± 0.04Y29A 1. 11 ± 0 .11 E31A 0.98 ± 0.06D32A 1. 08 ± 0.05Y29A-E31A 1. 04 ± 0.07D16A-Y29A-E31A 1. 18 ± 0.08*Y29W 1. 04 ... (%)XBSWild-type 10 0 ± 13 N54A 86 ± 19 G56A 70 ± 15 *N141A 81 ± 13 N181A 70 ± 21 T183A 63 ± 13 *W185A 62 ± 12 *N54A-G56A 68 ± 13 *N54A-T183A 65 ± 16 *N181A-T183A 66 ± 23G56A-T183A-W185A 54 ± 14 *N54F 83 ± 10 N54W ... 76 ± 17 N141Q 80 ± 26N54W-N141Q 79 ± 21 XANWild-type 10 0 ± 13 D16A 92 ± 6Y29A 82 ± 3E31A 70 ± 13 *D32A 10 1 ± 2D16A-E31A 76 ± 16 Y29A-E31A 73 ± 5*D16A-Y29A-E31A 82 ± 5*G15W 98 ± 12 D16Y...

Tài liệu Báo cáo khoa học: Dmrt1 genes at the crossroads: a widespread and central class of sexual development factors in ﬁsh pdf

... post-translational and functional regulations of the dmrt1a ⁄ dmrt1bY gene pairin medaka. 10 10 FEBS Journal 278 (2 011 ) 10 10 10 19 ª 2 011 The Authors Journal compilation ª 2 011 FEBSAmong the downstream candidate ... ﬁshFEBS Journal 278 (2 011 ) 10 10 10 19 ª 2 011 The Authors Journal compilation ª 2 011 FEBS 10 11 general rule, although some dmrt1 expression could bedetected in ovaries (see Table 1) . Interestingly, ... O (2 010 ) Sexually dimorphic expression of A. Herpin and M. Schartl Sexual development factors in ﬁshFEBS Journal 278 (2 011 ) 10 10 10 19 ª 2 011 The Authors Journal compilation ª 2 011 FEBS 10 17...

Tài liệu Báo cáo khoa học: Hsp105b upregulates hsp70 gene expression through signal transducer and activator of transcription-3 pdf

... hatayama@mb.kyoto-phu.ac.jp(Received 14 May 2009, revised 3 August2009, accepted 7 August 2009)doi :10 .11 11/ j .17 42-4658.2009.07 311 .xHsp105a and Hsp105b are mammalian members of the Hsp105 ⁄ 11 0 family,a divergent subgroup of ... shock transcription factors in regulation of the heatshock response and beyond. FASEB J 15 , 11 81 11 31. 18 Baler R, Dahl G & Voellmy R (19 93) Activation of human heat shock genes is accompanied ... control. How-ever, Hsp105b (1 6 41) , Hsp105b (1 5 91) , and Hsp105b (1 564), lacking more than the C-terminal 17 3 aminoacids of Hsp105b, failed to induce luciferase activity.As Hsp105b(D642–698), which...

Tài liệu Báo cáo khoa học: Studies on structural and functional divergence among seven WhiB proteins of Mycobacterium tuberculosis H37Rv pdf

... coefﬁcient of various WhiB proteinswas determined using vector-nti software. The values wereas follows: 17 550 m )1 Æcm )1 for WhiB1, 13 14 0 m )1 Æcm )1 forWhiB2, 18 830 m )1 Æcm )1 for WhiB3 ... A4200 15 030345060675750909 10 50 210 21 Effect of GSH (10 mM) 0 h 2 h 6 h 20 h 30 h 42 hWhiB7WhiB6WhiB5WhiB4WhiB3WhiB2WhiB1% Change in A420 15 0030345060675750909 10 50 210 21 Effect of dithiothreitol (10 ... each protein sample are expressed as means ± SD(three independent protein preparations).SamplesAtoms of ironper monomerWhiB1 (native) 0 .13 1 ± 0. 014 WhiB1 (in 8M urea) 0 .14 1 ± 0.052WhiB1 (refolded)...

Tài liệu Báo cáo khoa học: Top-down MS, a powerful complement to the high capabilities of proteolysis proteomics pdf

... University, Ithaca,NY 14 853, USAFax: +607 255 413 7E-mail: fwm5@cornell.edu(Received 30 May 2007, revised 12 October2007, accepted 17 October 2007)doi :10 .11 11/ j .17 42-4658.2007.0 614 7.xFor the characterization ... spectra of 1 : 1, 2 : 1 and 3 : 1 mixtures of 14 4 and 11 6 kDa proteinsshowed the corresponding molecular ions and, foreach, 11 17 different mass values of 1 10 kDa thatrepresented their b or y fragment ... highcapabilities of proteolysis proteomicsFred W. McLafferty 1 , Kathrin Breuker2, Mi Jin 1 , Xuemei Han 1 , Giuseppe Infusini 1 , Honghai Jiang 1 ,Xianglei Kong 1 and Tadhg P. Begley 1 1 Department of...

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