... fragment from the humantrypsinogen prepro sequence was amplified from humanpancreatic cDNA using the primer set (forward, CCCAAGCTTACCATGAATCTACTCCTGAT; reverse, GTTGGTACCTTGTCATCATCATCAAAGG), and ... 7(TACACACCCTGACCCGCATC) and 6 as template.Sequence analysis The sequence of the cDNA was analyzed using genetyxsoftware (Software Development Co. Ltd, Tokyo, Japan). A novel serine protease ... brain.Biochim Biophys Acta 1350, 11–14.11 Ogawa K, Yamada T, Tsujioka Y, Taguchi J, Takaha-shi M, Tsuboi Y, Fujino Y, Nakajima M, YamamotoT, Akatsu H, Mitsui S & Yamaguchi N (2000) Localiza-tion...