Báo cáo khoa học: Identifying determinants of NADPH speciﬁcity in Baeyer–Villiger monooxygenases docx

... 3-amine group of the coenzyme analog and theO4 of the isoalloxazine ring of the flavin cofactor, whichwould specifically promote AADP+binding. Such aligand–cofactor interaction is in line ... sequences contain two dinucleo-tide-binding sequence motifs (Rossmann-fold motifs) –GxGxxG – which are involved in binding of the ADPmoieties of FAD and NADPH [27]. This distinguishesthem ... Ó FEBS 2004This again confirms a role of Lys439 in coenzyme binding.By monitoring the redox state of the flavin during turnover of NADPH, it was found that the different amino acidsubstitutions...

Tài liệu Báo cáo khoa học: Molecular determinants of ligand speciﬁcity in family 11 carbohydrate binding modules – an NMR, X-ray crystallography and computational chemistry approach doc

... addition of CtCBM11. In general, a progressive line broadening of all thecellohexaose protons was observed during titration withincreasing amounts of protein, which can be understoodas a result of ... (Fig. 1III–V), which could indicate that the corre-sponding hydroxyl groups are involved in proteinbinding.The results for the linebroadening measurements of protons H1a in the alpha and beta conﬁgurations,aHa1 ... madamm software[22] that allows the introduction of a certain degree of protein ﬂexibility in standard docking processes.The process tries to mimic a conformational bindingmodel, in which...

Báo cáo khoa học: Structural determinants of protein stabilization by solutes The importance of the hairpin loop in rubredoxins pdf

... stabil-izing solutes reflects the strengthening of the hydrogenbond network. These findings are in line with an ear-lier study about the effect of DGP on the dynamics of Structural determinants of ... of Southampton, UK In spite of the extensive accumulation of data on pro-tein structure, the molecular determinants of proteinthermal stability remain elusive. Also, the beneficialstabilizing ... destabilized by addition of DGP. In con-trast, the presence of MG caused a consistent retarda-tion on the rates of all rubredoxins examined; in thecase of RdDg the increment of half-life induced byMG...

Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt

... becom-ing pupae in about a week after spinning (Fig. 1B).Isovaleric acid seems to be the cause of prepupallethality because injection of isovaleric acid intonormal spinning larva induces ... thesku mutant accumulates branched-chain amino acids in haemolymph, thismutant may be useful in the investigation of unique branched-chain aminoacid catabolism in insects.AbbreviationsACAD, acyl-CoA ... subtracting thereduction rate of the enzyme-excluded solution from that of the enzyme-containing solution and was expressed as nmols of 2,6-dichloroindophenol reduced per mg of protein permin....

Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: chemokines in the joints of patients pdf

... chemokines stimu-lated mainly by in ammatory cytokines, including IL-1b, TNF-a andIFN-c. (B) Chemokines expressed in the joint recruit leukocytes intothe joints. In addition to functioning in ... examination of affected rheu-matoid joints reveals extensive RANTES ⁄ CCL5expression in the synovial lining and sublining layers[22]. The expression of RANTES ⁄ CCL5 in culturedFLS increases in both ... IP-10, interferon-c-inducible protein-10; MAPK,mitogen-activated protein kinase; MCP, monocyte chemoattractant protein; Mig, monokine induced by interferon-c; MIP, macrophage in ammatory protein;...

Tài liệu Báo cáo khóa học: The lysozyme of the starfishAsterias rubens A paradigmatic typei lysozyme docx

... 1.cDNA cloning and sequencingcDNA was prepared from soft tissues of a single A. rubensspecimen. As a starting point, we used degenerate primersdesigned from the N-terminal sequence determined by ... peptide of 16 amino acids and its cleavage site. Ten more amino acidsare found in the predicted translated protein upstream of theserine that is found to be the N-terminal residue of theextracted ... struc-ture.During recent years, interest in a new type of lysozyme, theinvertebrate-type (i-type), has been growing. In 1996 Jolle`set al. [1] published the N-terminal sequences of lysozymesfrom two...

Tài liệu Báo cáo khoa học: The role of the ESSS protein in the assembly of a functional and stable mammalian mitochondrial complex I (NADH-ubiquinone oxidoreductase) pptx

... that the N-terminal domain is involved in protein–protein interactions with other hydrophilic domains of neighboring integral membrane subunits, and interspeciesinteractions are incompatible.From ... thebovine complex a large subdomain is made up of  20integral membrane proteins contributing > 60 transmem-brane segments. Some of these must be intimately involved in proton pumping. Another ... proteins reveals a high degree of conservation in the C-terminal domain (including thetransmembrane region), but a signiﬁcant number of differ-ences in the N-terminal domain (located on the matrix...

Tài liệu Báo cáo khoa học: "The Contribution of Linguistic Features to Automatic Machine Translation Evaluation" docx

... ﬁnding is that combining both kinds of metrics gives top performance according to ev-ery meta-evaluation criteria. In addition, our Com-bined System Test shows that, when training acombined ... Acceptable. In Proceedings of the Joint 21st International Conference on Com-putational Linguistics and the 44th Annual Meet-ing of the Association for Computational Linguistics(COLING-ACL), ... Role of BLEU in Machine Translation Research. In Proceedings of 11th Conference of the European Chapter of the As-sociation for Computational Linguistics (EACL).Chris Callison-Burch. 2005. Linear...

Tài liệu Báo cáo khoa học: "The Impact of Query Reﬁnement in the Web People Search Task" docx

... clustering task, with over 15 research groupssubmitting results in each campaign. Since the re-lease of the ﬁrst datasets, this task is becoming anincreasingly popular research topic among Infor-mation ... person. In a typicalhiring process, for instance, candidates are eval-uated not only according to their cv, but also ac-cording to their web proﬁle, i.e. information aboutthem available in the ... and n-grams beginning or ending with a stopword. Usingthe Stanford Named Entity Recognition Tool3weobtained the lists of persons, locations and organi-zations mentioned in each document.Additionally,...

Tài liệu Báo cáo khoa học: Electrostatic role of aromatic ring stacking in the pH-sensitive modulation of a chymotrypsin-type serine protease, Achromobacter protease I pdf

... thisprotease at pH9 [10]. Further interest in the aromaticstacking is in the role of the electrostatic properties in enzymatic catalysis of API, and in distinguishing thefunctionally catalytic ... andW169A, deviates from the original position. In the struc-tural deviation, the solvent ASA of the side-chain of His210increased with the decrease in size of the side-chain atresidue 169 (Table ... effect of the size of the residue at position 169 may berelated to the solvent accessibility of the side-chain of His210 (Fig. 3C). When the accessibility of the side-chain of His210 increased,...

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