Báo cáo khoa học: Monitoring the prevention of amyloid ﬁbril formation by a-crystallin Temperature dependence and the nature of the aggregating species pdf

Báo cáo khoa học: Utp25p, a nucleolar Saccharomyces cerevisiae protein, interacts with U3 snoRNP subunits and affects processing of the 35S pre-rRNA docx

... study by showing the direct interaction of Utp25p with SSU pro-cessome subunits, and the analysis of the 35S pre-rRNAcleavage reactions that are affected by the depletion of Utp25p. Furthermore, ... UTP-B and UTP-C[18,19]. t-UTP complex binds very early during tran-scription of the pre-rRNA, followed by the UTP-Bcomplex, U3 snoRNP and the Mpp10p complex, and later by Rrp5p and the UTP-C ... probes. The relative positions of the probes on the 35S pre-rRNA are indicated in (B).Bands corresponding to the major intermediates and to the mature rRNAs are indicated on the right-hand side. The...

Báo cáo khoa học: Monitoring ligand-mediated nuclear receptor–coregulator interactions by noncovalent mass spectrometry pptx

... different species on the ESI mass spectrum reﬂect the actual distribution of these species in solution. The reproducibility of the determination of the relative p roportions of the different species ... bind to the HD in the absence of any ligand, and (b) ESI-MS data allow one to directlymonitor the effect of ligand binding on the association/dissociation pattern of the heterodimer and the corepressor.RAR-speciﬁc ... of the species areobtained by summing the peak intensities of the three predominant charge states of the complexes. Reproducibility of the values is within 2–3%.No ligandRelative amounts of...

Báo cáo khoa học: Structural ﬂexibility in Trypanosoma brucei enolase revealed by X-ray crystallography and molecular dynamics pdf

... of the atoms. The eigenvalues reflect the magnitude of the fluctuationalong the respective EVs. Structural superpositions and other conformational analyses were performed using lsq-man [56] and ... represented, the former by PAH_1 and FPAH and the latter by PEP and PAH_2. This appears to be the first time that an enolase-PAH complex has crys-tallised in a nonfully closed conformation. The PAH_1 and ... the coordinated loop dimension(EV1) and the His156-out dimension (EV2). The tra-jectory reaches the EV1 value of the open sulfate_1*structure (maximum 1.73) and exceeds the EV2 values of the...

Báo cáo khoa học: Monitoring the prevention of amyloid ﬁbril formation by a-crystallin Temperature dependence and the nature of the aggregating species pdf

... Journal compilation ª 2007 FEBS 6305 Monitoring the prevention of amyloid fibril formation by a-crystallin Temperature dependence and the nature of the aggregating species Agata Rekas1,2, Lucy Jankova3, ... detail,two aspects of a-crystallin s fibril- suppressing ability: (a) its temperature dependence, and (b) the nature of the aggregating species with which itinteracts. First, the efficiency of a-crystallin ... decreased by 0.0167 gÆcm)3, from the start of the experiment. By the end of the measurement, the thickness had increased by 0.342 nm, the mass hadincreased by 0.10 ngÆmm)2 and the density...

Báo cáo khoa học: Carbohydrate binding sites in Candida albicans exo-b-1,3-glucanase and the role of the Phe-Phe ‘clamp’ at the active site entrance ppt

... implementedto help explain their importance to the speciﬁcity and catalytic efﬁciency of the enzyme. The role of the Phe-Phe entranceway The fortuitous discovery of an unreacted molecule of the mechanism-based ... solved the crystal structure of this GH5enzyme in the presence of two different mechanism-based inhibitors, thereby revealing the close network of interactions that hold the terminal glucose of the ... seen for the Phe triad in the structure of the mutant Exg:L3 complex. Neither of the two sugar residues interacting with the Trp triadoverlaps with the sugar seen in the Phe-Phe clamp of Exg.Structural...

Báo cáo khoa học: Cell death-inducing DFF45-like effector, a lipid droplet-associated protein, might be involved in the differentiation of human adipocytes pdf

... is oftencharacterized by an increase in both the size and the number of adipocytes. Previous data have shown thatadipose tissues play crucial roles in the development of obesity, and that the ... promote the formation of lipid droplets. However, the detailed roles of human CIDEC in the dif-ferentiation of human adipocytes remain unknown. In the present study,we found that the expression of ... 18 (a), 23 (b) and 33 (c) of gestation (scale bar = 25 lm). The expression of CIDEC increased along with the differentiation of adipose tissue, and the highest level of expression of CIDEC was...

Tài liệu Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase pdf

... peptide was identified by the almost complete series of y-fragments and a longseries of b-fragments (Fig. 4). From the difference of the precursor mass to the y-28 fragment and from the b1 fragment, ... labeling of P40within the gel by the CAF reagent occurred in a veryefficient way and allowed the identification of the new N-terminus after cleavage of the signal sequence(Fig. 5).Remarkably, the ... ions (…) and the b-fragment ions(– –). Calculated from the b1 fragment and the y 28 fragment, the N-terminal amino acid consists of asparagine modified by the CAF reagent(+136 Da). Most of the unlabelled...

Tài liệu Báo cáo khoa học: Changes in rat liver mitochondria with aging Lon protease-like activity and N e-carboxymethyllysine accumulation in the matrix doc

... high level of the polyunsaturated fatty acid linoleic acid [40,41]. Theyare prime target of ROS due to their location near to the site of ROS production [42] and could be the source of carbonyladducts. ... at )80 °C for furtherdeterminations of protease activity and the level of carboxymethylated protein. Protein was assayed by the Bradford method.To estimate the contamination of mitochondrial ... atoms of oxygen consumed per minute and per mg protein. The rate of oxygen consumption in state 3 and in state 4,respiratory control ratio (RCR) of oxygen consumptions instates 3 and 4 and the...

Báo cáo khoa học: Neuroserpin Portland (Ser52Arg) is trapped as an inactive intermediate that rapidly forms polymers Implications for the epilepsy seen in the dementia FENIB ppt

... calculate the inflection point of the transition and hence the Tm[14].Assessment of the polymerization of wild-type and Ser52Arg neuroserpinPolymerization of wild-type, Ser49Pro and Ser52Arg ... 24 h.Assessment of the conformation of Ser52Argneuroserpin The most likely cause for the inactivity of Ser52Argneuroserpin and inaccessibility of the reactive loop is that the mutant had adopted ... concentrations of urea, and the change influorescence profile was followed by exciting the protein at295 nm and measuring the shift in maximum fluorescenceFig. 5. Characterization of the c onformation of...

Báo cáo khoa học: Selecting cells with different Alzheimer’s disease c-secretase activity using FACS Differential effect of presenilin exon 9 deletion on c- and e-cleavage doc

... releases the activator domain in the cytosol, whichcan then traffic to the nucleus to initiate the transcription of the greenfluorescence protein (GFP) gene by binding to the 5Gal-E1b domain of the ... (kindly provided by F. Reinhard) and was cloned into pIRESpuro2. We thereby removed the N-terminal flag sequence. The last three mutations did notcontain the VRSQ-sequence and therefore their mutationswould ... aphosphorimaging screen and analyzed with the MACBAS V2.0 imaging software (Fuji) or exposed to BioMax MR-1film (Kodak) and the density of the bands quantified using the NIH-IMAGE1.60 software. For Ab–Western...

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