Báo cáo khoa học: The heat shock protein 70 molecular chaperone network in the pancreatic endoplasmic reticulum ) a quantitative approach potx

... centrifugation, and analyzed by SDS ⁄ PAGEand protein staining with Coomassie Brilliant Blue. The protein ladder was run on the same gel (lane 5). Pancreatic endoplasmic reticulum chaperone network A. ... major ER-lumi-nal heat shock protein (Hsp) 70, IgG heavy chain-binding protein (BiP), atleast one of which also involves the second ER-luminal Hsp70, glucose-reg-ulated protein (Grp) 170. In ... cysteine-rich region; TRX,thioredoxin-like domains). The quantitative aspects are summarized in Table 1.Table 1. Hsp70 chaperones and their cochaperones of the ER in the canine pancreas. The...

Báo cáo khoa học: Small heat shock protein Hsp27 prevents heat-induced aggregation of F-actin by forming soluble complexes with denatured actin docx

... small heat shock protein with molecular mass 25 kDa (hsp2 5) with actin. Eur JBiochem 270, 892–901.22 Brophy CM, Lamb S & Graham A (199 9) The small heat shock- related protein- 20 is an actin-associated ... before F-actin thermal denaturation (at30–35 °C) (A) and after F-actin denaturation (at 70 °C) (B). Condi-tions were the same as in Fig. 1A. Each plot is an average of 10distributions obtained ... heat- induced aggregation of F-actin. (A) F-actin (1.0 mgÆmL )1 ) washeated at a constant rate of 1 C°Æmin )1 in the absence (curve 1) or in the presence (curves 2– 4) of Hsp27-3D, and aggregation was fol-lowed...

Báo cáo khoa học: Structural and functional roles for b-strand 7 in the a-crystallin domain of p26, a polydisperse small heat shock protein from Artemia franciscana pdf

... 5¢-CACGTACAAAGAGAACGTCGACGACG-3¢5¢-CGTCGTCGACGTTCTCTTTGTACGTG-3¢R11 4A 5¢-GAGAATTTCGAGCACGATACAGACTCCC-3¢5¢-GGGAGTCTGTATCGTGCTCGAAATTCTC-3¢Y116D 5¢-CGACGACGAGACAGACTCCCAGAACATGTC-3¢5¢-GACATGTTCTGGGAGTCTGTCTCGTCGTCG-3¢Y. ... H, The ´riault JR & LandryJ (200 5) Structural instability caused by a mutation at a conserved arginine in the a- crystallin domain of Chinesehamster heat shock protein 27. Cell Stress Chaperones10, ... Stress Chaperones 8, 381–394. 70 Rajaraman K, Raman B, Ramakrishna T & Rao CM(200 1) Interaction of human recombinant aA- andaB-crystallins with early and late unfolding intermedi-ates of...

Báo cáo khoa học: Mapping of chorismate mutase and prephenate dehydrogenase domains in the Escherichia coli T-protein doc

... PDH, and induces aggregation of the T -protein [3]. An analogous bifunctional protein in E. coli,known as the P -protein, contains CM and prephenatedehydratase (PDT), and catalyzes the transformation ... Phe-binding domain,T -protein fragment analysis indicated that tyrosine bindingand feedback inhibition could not be attributed to a structural domain that was separate from the CM andPDH domains. Initial ... ofchorismate into phenylpyruvate in the biosynthetic pathwayto phenylalanine.Domain mapping studies on the P -protein (386 aminoacids, homodimer, molecular mass 43 kDa) have estab-lished that the...

Tài liệu Báo cáo khoa học: Accessibility changes within diphtheria toxin T domain when in the functional molten globule state, as determined using hydrogen⁄deuterium exchange measurements pdf

... chaincorresponding to the catalytic domain (C domain);and the B chain corresponding to the translocationdomain (T domain) and the receptor-binding domain. The C and T domains remain covalently linked by a disulﬁde ... using the following protein standards (Amersham): RNase (13.7 kDa), chymotrypsino-gen (25 kDa), ovalbumin (43 kDa), BSA (67 kDa) and BlueDextran (2000 kDa). BSA was removed from the standardsat ... of the toxin in the membrane. The C domainis then translocated across the endosomal membraneinto the cytosol. The C domain ADP-ribosylates the elongation factor 2, blocking protein translation...

Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx

... ferritin (DAKO); 5F6, a monoclonal antibody tooxidatively modiﬁed proteins containing the aldehydeadduct acrolein (a gift from K. Uchida8, Nagoya University,Nagoya, Japan). Rabbit anti-mouse and ... and stored at )7 0 °C for further analysis.From each sample, total RNA was isolated and DNAsetreated using the RNeasy kit according to the manufacturespeciﬁcations (Qiagen, Victoria, Australia). ... immunoprecipitation and mRNA studies. (A) Cytospins of HepG2 cells were incubated with Nor3.2 followed by rabbit anti-(mouse Igs) and the alkaline phosphatase-antialkaline phos-phatase (APAAP) conjugate,...

Tài liệu Báo cáo khoa học: High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine pptx

... particular, most of the essential features of the catalyticsite (i.e. a catalytic triad of Ser-His-Glu, an oxyanion hole, a p-cation-binding site, and an acyl-binding pocket) are the same in AChE and ... BuChE, as indicated. In each panel, the solid circlesindicate the measured kcatvalues, the solid squares indicate the measured bkcatvalues, and the lines are the result of ﬁtting the meas-ured ... positively chargedsubstrate) making protonation of the catalytic histidinemore difﬁcult.Plots of pK a values against the nonpolar surface area of the residue [31] or against any of a variety of...

Báo cáo khoa học: Leishmania donovani methionine adenosyltransferase Role of cysteine residues in the recombinant enzyme pptx

... 5¢-GTTCTCGAAGGTGTG TGCGAGCGAGTCGTCCGCGAAG-3¢(C44S), 5¢-GTTGCGTGCGAGTCGAGCGCGAAGACGGGCATG-3¢ (C47S), 5¢-CTG GACTACGAGTCGAGCAATGTGCTGGTTGCG-3¢ (C92S), 5¢-CAGTCGCCGGACATCAGCCAGGGTCTGGGCAAC-3¢ (C106S),5¢-GGCCTGGCGCGCCGCAGCCTTGTGCAGCTCGCG-3¢ ... Thermus aquaticus (Taq) poly-merase was from Promega and Pyrococcus furiosus (Pfu)polymerase was from Stratagene (La Jolla, CA, USA).Leishmania donovani promastigotes (insect ﬂagellated form)and ... decarboxylase inhibitor a- diﬂuoromethyl-ornithine (DFMO) leads to massive intracellular build-up ofAdoMet and a potential state of hypermethylation, causingcellular death in the parasite [14]. The information...

Báo cáo khoa học: Characterization of Trypanosoma brucei PEX14 and its role in the import of glycosomal matrix proteins pptx

... part of the GAPDH (a PTS-1 protein) ,ALD (a PTS-2 protein) and TIM (an I-PTS protein) isalready released from the cells at concentrations of digitoninthat leave the glycosomal membrane intact. ... no membrane-spanning domains in PEX14have been unambiguously identiﬁed and it was observed in mammalian cells and S. cerevisiae that both the N-terminaland the C-terminal domain are at the cytosolic ... compartmentation has been studied in detail in the kinetoplastid Trypanosoma brucei, the causativeagent of African sleeping sickness. When present in the mammalian bloodstream, this parasite...

Báo cáo khoa học: First evidence of catalytic mediation by phenolic compounds in the laccase-induced oxidation of lignin models ppt

... of catalytic mediation by phenolic compounds in the laccase-induced oxidation of lignin modelsFrancesca d’Acunzo and Carlo GalliDipartimento di Chimica, Universita`‘La Sapienza’, Roma, Italy; ... can be oxidatively converted into a reso-nance-stabilized phenoxy radical, performs as a mediator in the laccase-catalyzed oxidation of a nonphenolic substrate(4-methoxybenzyl alcohol) and also ... to argue that natural mediators may participate in the laccase-catalyzed oxidation of nonphenolic ligninsubunits in those micro-organisms that only rely on laccasefor their ligninolitic action...

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