Báo cáo khoa học: Altering the surface properties of baculovirus Autographa californica NPV by insertional mutagenesis of the envelope protein gp64 ppt

... the baculovirus envelope (Eur. J. Biochem. 269) 4467 Altering the surface properties of baculovirus Autographa californica NPV by insertional mutagenesis of the envelope protein gp64 Alexandra ... the native envelope protein gp64. Thereby, no duplication of the gp64 is necessary to target the foreign peptide to the surface of baculoviruses because each copy of the gp64 contains the target ... sites within the gp64 envelope protein wereselected by their theoretical maximum surface probabilityand investigated for eﬃcient peptide presentation. The ELDKWA peptide of the gp41 of HIV-1,...

Báo cáo khoa học: Spectroscopic and kinetic properties of the horseradish peroxidase mutant T171S Evidence for selective effects on the reduced state of the enzyme potx

... modified by mutation [14] or Ca-depletion [13] significant chan-ges in the properties of the ferric form of the protein has been detected. Even so, the changes detected in the haem cavity of the reduced ... in the disorder and hence entropy of the reduced state of the mutant compared to that of the wild-type protein. In contrast to the present study, in previous caseswhere the proximal site of ... isthat modification of the proximal cavity of HRPC by mutation or Ca2+ion removal has a significant impacton the properties of His170. The strength of the hydro-gen bond between the proximal His...

Báo cáo khoa học: Puriﬁcation and properties of the glutathione S-transferases from the anoxia-tolerant turtle, Trachemys scripta elegans pdf

... focusing of proteins.Methods Enzymol 22, 389–412.49 Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein- dye ... change in the amount of GST protein present, a covalent modiﬁ-cation of GST that alters its properties, or a change in the mixture of GST isozymes present in the organ tobetter suit the enzyme ... but did not ﬁndevidence of a change in the expression pattern of eitherisozyme during anoxia or of the expression of novelGST isozymes under anoxia. However, the effect of anoxia exposure on...

Tài liệu Báo cáo khoa học: Brain angiogenesis in developmental and pathological processes: regulation, molecular and cellular communication at the neurovascular interface pdf

... migrate through the extracellularmatrix with the help of matrix metalloproteinase [82]. The consequent event of brain macrophages is one of the most important defense mechanisms used by the brain. ... 2009)doi:10.1111/j.1742-4658.2009.07174.x The vascular network of the brain is formed by the invasion of vascularsprouts from the pia mater toward the ventricles. Following angiogenesis of the primary vascular network, ... by reducing the ﬂow of water into the brain [71].vSMCsvSMCs are myocytes that mediate vasoconstrictionand vasodilation. The thickness of the vSMC layer dif-fers according to the size of the...

Tài liệu Báo cáo khoa học: An ecdysteroid-inducible insulin-like growth factor-like peptide regulates adult development of the silkmoth Bombyx mori docx

... achieved.Determination of the structure of 8K-BLPMALDI-TOF MS analysis of the purified materialfrom the final HPLC yielded a monoisotopic mass of 7409.2 ([M + H]+) (Fig. 3A). Amino acid sequenceanalysis of the ... to those of bombyxin and insulin,which are of the order of 100 pm [23,24]. The veryhigh titer of 8K-BLP may be due to a remarkably highlevel of gene expression in the fat body and to the large ... alsoaffect the development of the wing disks at the prepu-pal stages. It is interesting to note that bombyxin-IIalso stimulated the growth of the wing disks in the butterfly Precis coenia and the hawkmoth...

Tài liệu Báo cáo khoa học: A tyrosinase with an abnormally high tyrosine hydroxylase/dopa oxidase ratio Role of the seventh histidine and accessibility to the active site docx

... be part of the defense sys-tem against wounding and infection by the pathogen[16]. Thus, the timing and location of melanizationseems to be essential for the prevalence of one of thesetwo ... 1.10.3.1), but the differentiation between these two types of enzymeis not clear [4]. Looking at the sequences of the twoenzymes, both show absolute conservation of the histi-dine residues of the CuA ... SDS.Fig. 3. Dependence of the TH activity of extracts of R3 and the two mutant strains on the presence of cofactor,L-dopa. (n) R3; (d)R3-0337–which only expresses the protein NP_519622; (m)R3-1501–which...

Tài liệu Báo cáo khoa học: "Mining metalinguistic activity in corpora to create lexical resources using Information Extraction techniques: the MOP system" doc

... classification task.7 The nai-ve Bayes (NB) algorithm estimates the conditional probability of a set of features given a label, using the product of the probabilities of the individual features ... /'YES'@[102]. The different number of positions considered to the left and right of the markers in our training corpus, as well as the nature of the features selected (there are many more ... threshold of similarity of 65% for comparison between a golden standard slot entry and the one provided by the application. Thus, if the autonym or the informational segment is at least 2/3 of the...

Tài liệu Báo cáo khoa học: Identi®cation and properties of type I-signal peptidases of Bacillus amyloliquefaciens doc

... for10 min a t either 3 0 °Cor42°C, the t wo cultures werelabelled for 1 min at the indicated temperature by the addition of 35S[methionine] (50 mCiáL)1) and chased by the addition of nonradioactive ... sequence-speci®ccleavage by SPases and removal of the s ignal peptide from the precursor (export) protein [4,6±8]. Minor differencesbetween individual signal peptides, speci®c properties of t heexport protein ... ielded a DNA fragment of 1.3 kb. The nucleotidesequence demonstrated the presence of three ORFs, and the deduced proteins to have 42, 73, and 82% of identity toFig. 2. Abundance of sipW- like DNA...

Tài liệu Báo cáo khoa học: "Discovering Global Patterns in Linguistic Networks through Spectral Analysis: A Case Study of the Consonant Inventories" pdf

... (i.e., the eigenvector correspond-ing to the largest eigenvalue) is the direction inwhich the sum of the square of the projections of the row vectors of the matrix is maximum. Infact, the principal ... threetaken together. The individual contribution of the other eigenvalues to the spectrum is signiﬁcantlylower than that of the top three. Third, the eigen-values on either ends of the spectrum ... Nevertheless, the work does notstudy the spectrum of the graph. The aim of the present work is to demonstrate the use of spectral analysis for discovering the global patterns in linguistic networks. These...

Báo cáo khoa học: Muramyl-dipeptide-induced mitochondrial proton leak in macrophages is associated with upregulation of uncoupling protein 2 and the production of reactive oxygen and reactive nitrogen species docx

... the safety of MB, the absence of undesirable effects on the central nervous system, and the lack of induction of inﬂammatory responses [22].Despite a long-standing interest in the ﬁeld of mura-myl ... immunomodulators to reveal the mechanism of action of toxic MDPs on mitochondrialrespiration by correlating the uncoupling effectinduced by these molecules with the level and function of UCP2 and free ... source of ROS production and the primary ROS generated issuperoxide anion as a consequence of monoelectronicreduction of O2. Moreover, the main sites of OÀ2genera-tion at the level of the...

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