... M.,Fujisawa,H.,Kleihues,P.&Walter,G.(2001)Reducedexpres-sion of the Aa subunit of protein phosphatase 2A in humangliomas in the absence of mutations in the Aa and Ab subunitgenes. Int.J.Cancer93, 798–804.31. Calin, G.A., De Lasio, M.G., Caprini, ... kDa instead of the calculated 72 964 Da, we verified the correctness of the expected initiation methionine by translating the fusionprotein GST-XN73 and His6-XN73. As can be seen in Fig. 2B, these ... fusion proteins run at the expected height,proving that no internal methionine was used as initiationstart in the translation of the wild type mRNA of XN73 and that the XN73 protein runs with...