Báo cáo khoa học: NADH oxidation and NAD+ reduction catalysed by tightly coupled inside-out vesicles from Paracoccus denitriﬁcans doc

... NADH oxidation and NAD+ reduction catalysed by tightly coupled inside-out vesicles from Paracoccus denitrificans Alexander B. Kotlyar and Natalia BorovokDepartment ... Israel Tightly coupled inside-out vesicles were prepared from Paracoccus denitrificans cells (SPP, sub -Paracoccus particles) and characterized kinetically. The rate of NADH oxidation, catalysed by ... suppressing the rate of NADH oxidation of SPPFig. 1. Kinetics of NADH oxidation and NAD+reductionbySPP.Lineweaver–Burk plots of the initial rates at different concentrations of NADH (A) and NAD+(B)....

Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

... and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of1 nm. Thermal stability of Rep245 was measured by incu-bating the protein at 60, 70 and 80 °C for 30 min and ... Na-acetate (pH 5.0, 5.4 and 5.8),Tris ⁄ HCl (pH 6.5, 7.0, 7.5 and 8.0) and glycine ⁄ NaOH(pH 8.6, 9.0 and 9.6). Reactions were carried out for30 min at 65 °C and the products resolved by denaturinggel ... bacterio-phages and crenarchaeal and Gram-positive bacterialplasmids. In a recent description, they are said to betypiﬁed by the RepA-like protein ORF904 encoded by the pRN1 plasmid from the hyperthermophilic...

Tài liệu Báo cáo khoa học: Computational processing and error reduction strategies for standardized quantitative data in biological networks doc

... could be eliminated by randomized sample loading, and error reduction wasachieved by the use of normalizers or calibrators incombination with computational data processing. By converting relative ... generated under standardized conditions iscritical for understanding dynamic cellular processes. We report strategiesfor error reduction, and algorithms for automated data processing and forestablishing ... the gel and the transfer procedure leading to correlatederrors. These correlations are prevented by randomized gel loading, whichsigniﬁcantly reduces standard deviations. Further error reduction...

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon doc

... sequencing and MALDI-TOF MS analy-sis. This work was supported partly by a CreativeScientific Research from the Japan Society for the Pro-motion of Science (JSPS-13GS0023) and partly by anIntellectual ... acetylornithine and glutamate to formacetylglutamate and ornithine, thereby functioning in the first and fifthsteps in citrulline biosynthesis. CLGAT enzyme purified 7000-fold from leaves was ... with those from At2g37500 from A. thaliana (A),B. subtilis GAT (B) and S. cerevisiae GAT(S) (accession nos. AAC98066, NP389002, and NP012464, respectively). Consensus identical (black) and similar...

Tài liệu Báo cáo khoa học: "Identifying Agreement and Disagreement in Conversational Speech: Use of Bayesian Networks to Model Pragmatic Dependencies" docx

... between A and Bnumber of spurts between A and Bnumber of spurts of speaker B between A and Bdo A and B overlap?Durational features:duration of Aif A and B do not overlap: time separating A and Bif ... “yes” and “right”, as listedin (Cohen, 2002), general cue phrases, e.g. “but” and “alright” (Hirschberg and Litman, 1994), and adjectives with positive or negative polarity (Hatzi-vassiloglou and ... present both in A and B (webuilt 3 features for ranging from 2 to 4)ﬁrst and last word of Anumber of instances at any position of A ofeach cue word listed in (Hirschberg and Litman,1994)does...

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a sialic acid speciﬁc lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

... an important component of the ligands recognized by thelectins. Recognition can be affected by specific structuralvariations and modifications of sialic acids and their linkageto the underlying ... antennarius [12], Scylla serrata [24] and Penaeus monodon [27]. The lectin from Scylla serratawas NeuGc specific [24] and that from Cancer antennariuswas 9-O-NeuAc and 4-O-NeuAc specific [12,28]. Lectinswith ... sialidasetypeX,proteaseenzymes and molecular mass standards were purchased from Sigma.Preparation of crab seraFreshwater field crabs, Paratelphusa jacquemontii werecollected from the local wetlands of Kanyakumari...

Báo cáo khoa học: Comparative biochemical and functional studies of family I soluble inorganic pyrophosphatases from photosynthetic bacteria potx

... This work wassupported by research grants from the Spanish(BMC2001-563 and BFU2004-00843, MEC) and Andalusian Regional (PAI group CVI-261) Adminis-trations, funded in part by the EU FEDER program.References1 ... UÆmg)1protein and recovery levels of 20–30%. In all cases, the analysis by SDS ⁄ PAGE and native PAGE of puriﬁed preparations showed onlyone protein band of 20–22 kDa (Figs 1 and 2; supple-mentary ... relationshipbetween cyanobacteria and heliobacteria determined by phylogenetic analysis of photosynthetic genes [25].No protein bands were immunodetected in cell extracts from E. coli K12 and DH5a or nonphotosynthetic...

Báo cáo khoa học: Puriﬁcation and characterization of Helicobacter pylori arginase, RocF: unique features among the arginase superfamily doc

... tetracycline and ampicillin and induced with isopropyl thio-b-D-galactoside. Bacteriawere harvested and lysed by French Press. The whole cell lysate (WCL)was separated into soluble and insoluble ... (Figs 3, 4 and 6 and Table 1), and (e) the rocF gene and RocF protein shared homologywith the arginase/agmatinase superfamily [8]. These resultsdemonstrate that rocF is necessary and sufﬁcient ... manganese, inhibition by reducingagents, and a pH optimum considerably lower than that ofpreviously characterized arginases.Materials and methodsBacterial strains, growth conditions, and plasmidsE....

Báo cáo khoa học: Puriﬁcation and characterization of novel salt-active acharan sulfate lyase from Bacteroides stercoris HJ-15 ppt

... activity was potently inhibited by Cu2+, Ni2+ and Zn2+. This enzyme was inhibited by some agents, butanediol and p-chloromercuric sulfonic acid, which modify arginine and cysteine residues. The ... sulfate lyases and heparinase III, from B. sterocoris were not activated by salts.Therefore, to purify salt-active heparin lyase, B. stercorisHJ-15 cells were disrupted by ultrasonic, and the super-natant, ... activity was slightly increased by addition of Mn2+, whereas they were severely inhibited by Cu2+, Ni2+ and Zn2+(Table 2). The puriﬁed enzymewas inhibited by PCMS3 and butane-1,3-diol. Particularly,PCMS...

Báo cáo khóa học: Isolation and biochemical characterization of two soluble iron(III) reductases from Paracoccus denitriﬁcans docx

... Fe(III) sulfate by NADH catalyzed by cytosolic and membrane fractions. The former reaction differed markedly from the latter by about 10-times lower apparent Kmvaluefor NADH, by a discernible ... enzymes (FerA and FerB) catalyzing the reduction of a number of iron(III) complexes by NADH, were puriﬁed to near homogeneity from the aerobicallygrown iron-limited culture of Paracoccus denitriﬁcans ... ofFe(II) from the Fe(III)–siderophore complex upon incuba-tion with NADH, succinate and an ultracentrifuged and dialyzed cell-free extract while Dailey and Lascelles [25]noticed reduction...

Xem thêm