Báo cáo khoa học: Crystal structure of heme oxygenase-1 from cyanobacterium Synechocystis sp PCC 6803 in complex with heme pot

Báo cáo khoa học: Crystal structure of heme oxygenase-1 from cyanobacterium Synechocystis sp. PCC 6803 in complex with heme pot

... Crystal structure of heme oxygenase-1 from cyanobacterium Synechocystis sp. PCC 6803 in complex with heme Masakazu Sugishima1, Catharina T. Migita2, Xuhong Zhang3, ... fer-redoxin; HO, heme oxygenase; heme HO, H O in complex with heme; Syn HO-1, HO-1 from Synechocystis sp. PCC 6803; HmuO, HO from Corynebacterium diphtheriae; HemO, HO from Neisseria meningitidis.Enzyme: ... utilizing reducing equiv-alents [1]. In mammals, HO is mainly involved in heme metabolism f or the purpose of recovering iron from waste heme. Biliverdin IXa is further reduced by biliverdinreductase...

Báo cáo khoa học: Crystal structure of archaeal highly thermostable L-aspartate dehydrogenase/NAD/citrate ternary complex doc

... stereospeciﬁcity for hydrogenremoval from the C4 position of the nicotinamide moi-ety of NAD(P)H. In our binding model, the re-face of the nicotinamide ring is in front of the a-hydrogenatom of ... using insight ii (Biosym ⁄ MSI, San Diego, CA).Within this structure, two oxygen atoms of the C a car-boxylate are situated within hydrogen- and ionic-bond-ing distance of the side chains of ... of l-aspartate to iminoaspartate, after which QS catalyzesthe condensation of iminoaspartate with dihydroxyace-tone phosphate to produce quinolinate. Quinolinate isthen converted to nicotinate mononucleotide...

Báo cáo khoa học: Crystal structure of Thermoanaerobacter tengcongensis hypoxanthine-guanine phosphoribosyl transferase L160I mutant ) insights into inhibitor design potx

... Xiaocheng Gu1, Ming Luo1,3and Xiaofeng Zheng1,21 National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing, China2 Department of Biochemistry and ... con-taining 0.4 mL of the respective crystallization solution. Tofully saturate the enzyme binding site, GMP was added in a4 : 1 molar ratio with respect to wild-type and mutantHGPRTs. Single crystals ... to crystallization screening. The crystal structure of one mutant, L160I, was determined at 1.7 A˚resolution.Surprisingly, the active site is occupied by a peptide from the N-terminus of a...

Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt

... Kristiina Kruus2,Juha Rouvinen1and Nina Hakulinen11 Department of Chemistry, University of Eastern Finland, Joensuu, Finland2 VTT Technical Research Centre of Finland, Espoo, FinlandKeywordsascomycete; ... Joly N,Madzak C & Mougin C (2002) Crystal structure of afour-copper laccase complexed with an arylamine:insights into substrate recognition and correlation with kinetics. Biochemistry 41, ... 507–513.50 Krissinel E & Henrick K (2005) Detection of proteinassemblies in crystals. In Lecture Notes in Computer Sci-ence (Berthold MR ed), pp 163–174. Springer-Verlag,Berlin.51 Krissinel E...

Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt

... determined the X-ray crystal structure of a truncated form of importin-a lacking the importin-b binding domain, bound to a CLIC4NLS peptide. The NLS peptide binds to the major binding site in anextended ... gauge crystal quality before autoindexing anddetermining the optimum rotation range using strategy from within mosflm [35]. Crystals were found to have thesymmetry of the orthorhombic space ... obtain the importin-a:CLIC4NLS complex lacks the ﬁrst 69 residues that corre-spond to the ﬂexible importin-b binding domain. Theimportin-b binding domain is known to have anautoinhibitory function,...

Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... metalcofactor, respectively. SODs are grouped into fourclasses according to their metal cofactors: copper andzinc-containing SOD (Cu,Zn-SOD), iron-containingSOD (Fe-SOD), manganese-containing ... cambialistic SODs. In addition to the tertiarystructures of metal-speciﬁc SODs [4], crystal structures of several cambialistic SODs have been reported,including those from Porphyromonas gingivalis [5] ... determining the oligomeric struc-ture of SOD enzymes.We observed ﬁve-coordinate and six-coordinate struc-tures of metal ions in Mn-bound and Fe-bound Ape-SOD crystal structures, respectively. In...

Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc

... (2003) Crystal structures of human prostatic acid phosphatase in com-plex with a phosphate ion and a-benzylaminobenzyl-phosphonic acid update the mechanistic picture andoffer new insights into inhibitor ... phytate-bound structure of AppA,even in the absence of sulfate ions. The side chain of the corresponding Glu212 bends out of the cata-lytic pocket of PhyK avoiding steric or charge inter-actions with ... ancestor(‘prototype’) of HAPs, endowed with the potential todevelop speciﬁc enzymatic features in response to selec-tive pressures arising from individual environmentalconditions. According to the crystal structures...

Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx

... DESC subfamily in complex with benza-midine. The proteinase domain of DESC1 exhibits a trypsin-like serine pro-teinase fold with a thrombin-like S1 pocket, a urokinase-type plasminogenactivator-type ... thecatalytic machinery and structures required for binding of the main chain of the substrate peptide and properpositioning of the scissile bond with respect to the cata-lytic serine. Speciﬁcity ... matriptase in complex with benzamidine, (C) human hepsin in complex with the inhibitor 2-(2-hydroxy-phenyl)1H-benzoimidaxole-5-carboxamidin and (D)human enteropeptidase in complex with thetrypsinogen...

Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc

... determination of the BcZBPprotein at a resolution of 1.8 A˚, provides importantclues towards understanding the enzyme function,including the identiﬁcation of a zinc-binding site withina potential ... proteins. B. cereus thus provides a convenient model organism forstudying proteins potentially associated with the pathogenicity of the highlyinfectious B. anthracis. The zinc-binding protein of ... bdomain. The overall structure can be divided into twodistinct structural motifs shown with different shades of gray in the topology diagram of Fig. 1A. The N-ter-minal part of the protein (residues...

Tài liệu Báo cáo khoa học: Crystal structure of a subtilisin-like serine proteinase from a psychrotrophic Vibrio species reveals structural aspects of cold adaptation docx

... Thermoactino-mycetes vulgaris.ResultsThe crystal structure of the Vibrio proteinaseThe obtained Vibrio proteinase crystals formed clusters of needles, which transformed into thin platelets withina ... ongoing mutagenic research in which single and combined amino acid substitutionsaimed at increasing the stability of the Vibrio protein-ase are being tested. Elucidation of the Vibrio protein-J. ... Homology modelling and protein engineeringstrategy of subtilases, the family of subtilisin-like serineproteinases. Protein Eng 4, 719–737.39 Schechter I & Berger A (1967) On the size of the activesite...

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