structure of rpa the crystal structure of rpa p70 from residues 181 422 is represented in the absence 1a or presence 1b of a dc 8 dna substrate the structure was analyzed using pymol analysis of the pbd file 1fgu 4a represents rp
... outstanding volleyball player special physical evaluation, research with the team, the test data ofthe indicators, using SPSS13.0, Microsoft Excel software for data Statistical AnalysisThe purpose ... so as to work out the best athletes physical training program, but also for the theoretical system of sports training in Vietnam improve and supplement of great significance The article use of ... indicators, using SPSS13.0, Microsoft Excel software for data Statistical Analysis This paper the use of literature, expert interviews, statistics and questionnaire method, screened Vietnam outstanding...
... PAI-1(I13 7A) , PAI-1(I13 8A) , PAI-1(N13 9A) , PAI-1(W1 4 1A) , PAI1(T14 6A) and PAI-1(M149K) Structural analysis Structural analysiswas based on the following depositions inthe Protein Data Bank ([32]; ... PAI-1, and thus in an inert, probably latent conformation (Fig 2) The rate of latency transition was determined for all variants Typical assays are depicted in Fig 4, and the data for all the variants ... amount of PAI-1 required to inhibit half the uPA The half-life of PAI-1 was finally calculated from an exponential decay plot ofthe data obtained Generally, only one preparation of each PAI-1 variant...
... cervical to thoracic tracheal area Coughing and dyspnea disappeared and the dogs resumed normal activity Stent implantation isa minimally invasive, effective, easy and brief procedure that can ... used inthe treatment of tracheal collapse ina dog J Am Vet Med Assoc 2004, 225, 1217-1221 10 Moritz A, Schneider M, Bauer N Management of advanced tracheal collapse in dogs using intraluminal ... the lumen is obliterated on lateral thoracic radiograph M: male, F: female, FS: neutered female, CT‐TT: cervical trachea to thoracic trachea Fig Lateral radiographs before (a) and after (b) intratracheal...
... mg/mL paracetamol added to the drinking water The abdominal aorta was accessed surgically by a midline abdominal incision using sterile technique The retroperitoneum was explored and the aorta was ... “Principles of Laboratory Animal Care” formulated by the National Society for Medical Reseacrh and “Guide for the Care and the Use of Laboratory Animlas” prepared by the US Natinoal Academy of ... three sections: the proximal, intact part ofthe aorta, the wounded part ofthe aorta, and the distal, intact part ofthe aorta Following the dehydration process using graded ethanols, specimens...
... patient was readmitted with unstable angina pectoris (NYHA IV) A third attempt to reengage the protruding stent inthe vein graft ostium was discussed and planned The protruding part ofthe aorta was ... engage the stent inthe vein graft ostium was performed Unfortunately, the engagement was unsuccessful and the procedure was aborted Another conservative approach was proposed and a PCI ofthe ... GJ and RA analyzedthe procedure describe in this manuscript LP and RA wrote the manuscript All authors read and approved the final manuscript References Satler LF: Aorto-ostial disease and aorto-ostial...
... exhale a maximal amount of air while a signal fromthe force transducer produced a readable analog wave The most stable waveform was selected, and the mean value was recorded Subjects performed ... longer than minutes Training was conducted at the Mayborn Campus Center (MCC) at the University of Mary Hardin-Baylor under the supervision of trained research assistants, documented in training logs, ... as means ± standard deviations All statistical procedures were analyzedusing SPSS (Statistical Package for Social Science) version 16.0 Results Medical Monitoring, Dietary Analysis, and Training...
... disease in China in 1 981 Nearly 30 years later, this disease has now spread widely across China and affects almost all ofthe mink cultivation sites [24] In recent years, although a vaccine has ... residuesinthe vicinity of residue 300 ofthe amino acid sequence can reduce the amount of adsorption ofthe virus into canine cells [39] Inthe late 1 980 s and early 1990 s, the original CPV- 2a and ... be variable in VP2 proteins of nine MEV strains listed in table 1, and these include in particular the amino acid residuesinthe vicinity of residue 300 (Table 1) This may lead to changes in...
... A sample writing lesson plan usingthe genre approach Unpublished thesis, RELC, Singapore 25 Thi Kim Anh, Dang (2002) Applying a genre-based approach in teaching the writing ofthe English Discussion ... 2 Aims and objectives ofthe research The research is aimed at investigating students‘ attitudes towards the genre-based approach, which was new to them, and examining whether this teaching approach ... where the research was conducted and the informants involved inthe research It includes the writing tasks inthe textbook, research design, the teaching cycle inthe study The method of data collection...
... even inthepresenceof phosphate M240 is an invariant, solvent inaccessible residue (ASA ẳ A2 ) that anchors the short helix a8 Â to the core ofthe protein [1] Merz et al [36] have shown that disruptive ... residue that anchors the short helix a8 Â to the core ofthe protein [1] This proposed disulde crosslinks the loops b 1a1 and b 8a8 , which are widely separated in sequence but adjacent in space (Fig ... probes ofthe folding pathway of barnase: increasing the stability of proteins against the rate of denaturation Biochemistry 32, 43224329 Sambrook, J., Fritsch, E.E & Maniatis, T (1 989 ) Molecular...
... persists stably inthepresenceof DNA, whereas this fragment is rapidly degraded intheabsenceofDNA Several smaller fragments are produced in relatively large amounts intheabsenceof DNA, ... whereas these peptides are practically absent inthepresenceofDNAThe data suggest thepresenceof an inner folded core, which is suggested to participate inDNA binding, on the basis of DNA- binding-induced ... cleavage along motifs 1A and 1B, indicating either DNA- binding-induced conformational changes or covering of otherwise exposed proteolytic sites by DNA binding to these segments Motif 1Ais dispensable...
... De Luca [16] These authors developed a statistical-biomechanics method for analyzing force platform data recorded during quiet standing, called stabilogram diffusion analysis (SDA) SDA was applied ... volume, and left/right balance modulation can be found in [9] Each subject was instructed to maintain balance during the trials by taking advantage ofthe ABF information, when available Five trials ... behaviour of subject #8 COP-RMS percentage change using ABF is reported on the horizontal axis and F95% percentage change using ABF is reported on the vertical axis The values of each subject from...
... indicate a clear advantage ofthe mimetic scheme Inthe same table at the bottom, the numerical convergence rates for each method are also presented A quadratic convergence rate was obtained for the ... equations (4.1) is too general for the purpose of our theoretical analysis Therefore two simplifications are in order They are the assumption that K isthe identity operator and the restriction ... presented Theoretical and numerical analysisof its quadratic convergence rate isa new contribution This is not an obvious result in view ofthe first-order truncation errors and the nonstandard linear...
... attendance and several training sessions The inter-rater agreement was assessed using Intraclass correlation ICC(2,1) The ICC was 89 8 for the PCL-R total score; 735 for factor and 920 for factor scores ... factor model, 83 for facet 2/factor inthe three factor model, 87 for facet 3/factor inthe three factor model, and 64 for facet Statistical analysis Data analyses were made with SPSS 16.0 statistical ... design, and acquisition of data, analyzed and interpreted data, and served as first author GW-H contributed to conception, design, and acquisition of data, analyzed and interpreted data, and participated...
... process and data analysis Thanks to Giorgio Saragò M.D., and Stefania Bini M.D for help in administering the NPPO-REAC treatment and Alessandro Castagna M.D for his helpful support Author details ... design and coordination and in drafting ofthe manuscript LA has critically revised the manuscript and PM has done the psychiatric evaluation All authors read and approved the final manuscript ... activate REAC and inthe other the placebo-REAC The physicians that have participated inthe treatment’s administration turned inthe different rooms, using both “active” and “inactive” REAC but they...
... China, using DNAstar software package Sequence analysis based on the complete ORF2 sequence ofthe isolate in this study and other 10 genotype HEV isolates in China, using DNAstar software package ... transmission in this area Competing interests The authors declare that they have no competing interests Authors' contributions All authors participated inthe planning ofthe project XGH wasthe ... whether this genotype HEV strain is prevalent in Shanghai swine or not HEV paly an important role in acute sporadic and epidemic viral hepatitis in Asia and Africa Although HEV infection is frequent...
... after disposal Therefore, accurate prediction ofthe settling processes of disposed material in open waters, such as the descending and transport or dispersion ofthe material, isof practical ... stratification can be induced by many natural or man-made processes For instance, ocean disposal operations of dredged material usually occur inthe dredging of navigation channels and harbors, ... component in z-direction vi x* y* z* t * x y σ t Z spatial and temporal coordinates in physical domain spatial and temporal coordinates in computational domain dumping distance Greek Symbols β weighting...
... PhyK inthea ⁄ b domain than inthea domain [20] This is also reflected inthe rigidity ofthe protein The atomic displacement factors ofthe PhyK structure are smaller for thea ⁄ b domain than ... protein chain is shown Colors are the same as in Fig 1A indicating the two domains (B) Electrostatic surface potential ofthe active site of PhyK as calculated with APBS [ 38] is displayed ina range ... 3-phosphate The 3-phosphate isin close contact with Arg 28 Therefore, these two conserved arginine residues are important for arranging thesubstrateinthe correct orientation for catalysis, whereas...
... [ 38] in CCP4i, using proteinase K (PRK; PDB 1ic6 [20]), as search model Thestructurewas built usinga combination of auto building in ARP ⁄ wARP [39] and manual refitting of side chains using ... strong binding site found in PRK and formed by the side chain of Asp200 and the carbonyl oxygen atoms ofresidues 175 and 177 and is involved in stabilizing strands b8 and b9 Ca4 isthe weak binding ... density was observed inthe prime site ofthesubstrate binding region of SPRK This was initially interpreted as a Pro-Ser dipeptide, but was after a few cycles of refinement interpreted as an Ala-Pro-Thr...
... residues at the interface coming fromthe PYR and PP domains This isin agreement with the average mobility ofthe domains inthe crystal; the PYR domain has the lowest average B-factor, ˚ 23 A2 (comparable ... explain the lack ofsubstrate activation in ZmPDC In IPDC, the assembly ofthe subunits inthe tetramer resembles that of ZmPDC rather than ScPDC As in ZmPDC, the active site loops are folded in ... folded ina conformation poised for catalysis even intheabsenceofsubstrateor other activators Thestructureof IPDC supports the conclusion that thesubstrate activation observed in most PDC species...