Báo cáo khoa học: Differences in substrate speciﬁcities between cysteine protease CPB isoforms of Leishmania mexicana are mediated by a few amino acid changes potx

... speciﬁcities between cysteine protease CPB isoforms of Leishmania mexicana are mediated by a few amino acid changes Maria A. Juliano1, Darren R. Brooks2, Paul M. Selzer3, Hector L. Pandolfo1, Wagner ... & Juliano, M .A. (2001) S1subsite speciﬁcity of a recombinant cysteine proteinase, CPB, of Leishmania mexicana compared with cruzain, humancathepsin L and papain using substrates containing ... peptidase upon transfer into the endoplasmic reticu-lum ,a1 06aminoacidpro-region ,a2 18aminoacidmaturedomain that includes the active site, and a C-terminaldomain of either 16 or 100 amino acids [7]....

Báo cáo khoa học: Comparing the substrate speciﬁcities of cytochrome c biogenesis Systems I and II docx

... c552cleaved of its signal peptide is approx-imately 9 kDa, whereas the uncleaved product has a mass of approximately 11 kDa.Maturation of single -cysteine holocytochromesThere are natural examples ... and CcmB are not involved in hemetransport in E. coli [44,45]. Notably, maturation of anAXXAH-containing variant b -type cytochrome c550 in the present study indicates a nascent heme-binding ... spectra are vertically offset for clarityand normalized for wet cell weight. Samples were reduced by theaddition of a few grains of disodium dithionite. The absorbancemaxima are at 417, 521 and...

Báo cáo khoa học: Probing the substrate speciﬁcities of matriptase, matriptase-2, hepsin and DESC1 with internally quenched ﬂuorescent peptides potx

... basic (Arg) oracidic (Glu) amino acids. Position P1 was always occu-pied by Arg because TTSPs have an exclusive prefer-ence for substrates that contain this amino acid (orLys) [2]. Amino acids ... (Shanghai, China).Aprotinin, leupeptin, AEBSF, soybean trypsin inhibitor,pepstatin, bestatin and E-64 were from Roche Diagnostics(Laval, Canada). EDTA, ortho-phenanthroline and heparinwere ... required a basic amino acid (Arg) at the P4 position of the substratesto establish kcat⁄ Kmvalues. The presence of other types of amino acids at this position (Ala, Glu, Leu andTyr; substrates...

Báo cáo khoa học: Predicting the substrate speciﬁcity of a glycosyltransferase implicated in the production of phenolic volatiles in tomato fruit pptx

... FragariaxananassaUGT78D1 A. thalianaUGT8 6A1 A. thalianaUGT8 7A1 A. thalianaUGT8 3A1 A. thalianaUGT8 2A1 A. thalianaUGT8 5A1 A. thalianaSbHMNGT S. bicolorUGT76D1 A. thalianaUGT76E1 A. thalianaS39507 ... thalianaOsSGT1 O. sativaUGT74F1 A. thalianaUGT74F2 A. thalianaNtGT2 N. tabacumUGT75C1 A. thalianaUGT75B1 A. thalianaUGT75D1 A. thalianaUGT84B1 A. thalianaUGT8 4A1 A. thalianaFaGT2 FragariaxananassaUGT78D1 ... lycopersicumUGT76F1 A. thalianaCAO69089 V. viniferaUGT76B1 A. thalianaUGT76C1 A. thalianaUGT71B1 A. thalianaCaUGT1 C. roseusUGT71C1 A. thalianaUGT71D2 A. thalianaUGT8 8A1 A. thalianaUGT72E2 A. thalianaUGT72E3...

Tài liệu Báo cáo khoa học: Changes in purine speciﬁcity in tandem GAF chimeras from cyanobacterial cyaB1 adenylate cyclase and rat phosphodiesterase 2 pptx

... of amino acidstowards the N-terminal, two amino acids at a time, toidentify the amino acids that impeded cAMP stimula-CyaB2-GAFB-cAMPrPDE 2A- GAF-B-cGMP CyaB2-GAF -A- cAMP CyaB2-GAF-B-cAMP A ... the tandemGAF domain of cyaB1 in which only GAF B appearsto mediate signalling to investigate ligand speciﬁcation. In fact, the interacting amino acids, Arg256 andThr258, are conserved in the ... theN-terminal domains. Five PDE families, 2, 5, 6, 10and 11, have an N-terminal tandem GAF domain of about 500 amino acids, i.e. two GAF domains, termed A and B, are sequentially connected by a short...

Báo cáo khoa học: pH dependence, substrate speciﬁcity and inhibition of human kynurenine aminotransferase I ppt

... efﬁcient in catalyzing the transamination of a number of amino acids, including glutamine, phenylalanine,leucine, kynurenine, methionine, tyrosine, histidine, cys-teine, and aminobutyrate (Table ... I/glutaminetransaminase K (EC 2.6.1.64; KAT-I) is a multifunctionalenzyme. In vitro, the enzyme catalyzes the t ransamination o fseveral amino acids (e.g. glutamine, methionine, aromatic amino acids ... onhKAT-I catalyzed KYNA formation. Analysis of substrate speciﬁcity revealed t hat a number of amino acids a nd a- ketoacids can serve as the amino group donor and acceptor,respectively, for hKAT-I....

Tài liệu Báo cáo khoa học: Structural and functional speciﬁcities of PDGF-C and PDGF-D, the novel members of the platelet-derived growth factors family docx

... encom-passing almost only the CUB domain, and the longervariant containing the CUB domain and the ﬁnal 30residues in the C-terminal end of the growth factordomain. These splice variants are also ... revealing a remarkable increase in contact between the prostate carcinoma cells and the stromalcells, which is critical for prostate cancer progressionand metastases [66]. As human prostate cancer ... tumour angiogenesis. The apparent roles of PDGF-C in diseases and malignancies are discussedbelow and are summarized in Fig. 5.PDGF-C in Ewing family sarcomasThe ﬁrst link between PDGF-C and malignancy...

Báo cáo khoa học: Molecular basis for speciﬁcities of reactivating factors for adenosylcobalamin-dependent diol and glycerol dehydratases pptx

... enzyme-bound, adenine-lacking cobalamins for free adenosyl-cobalamin, an adenine-containing cobalamin throughintermediary formation of apoenzyme. The function of reactivating factors is to release a tightly ... that reactivating factor mediates theexchange of the enzyme-bound, adenine-lacking cobal-amin for free, adenine-containing cobalamin toward a cognate dehydratase. In addition, DDR can mediate ... consideredmodels of the damaged cofactor (adenine-lackingcobalamin) and intact coenzyme (adenine-containingcobalamin), respectively. As shown in Fig. 3A, whenthe diol dehydratase–CN-Cbl complex was incubatedwith...

Tài liệu Báo cáo khoa học: Molecular and cellular speciﬁcity of post-translational aminoacyl isomerization in the crustacean hyperglycaemic hormone family docx

... PR, Saccamo NA,Thadeio PF & Volkmann RA (1996) Posttranslational amino acid epimerization: enzyme-catalyzed isomeriza-tion of amino acid residues in peptide chain. Proc NatlAcad Sci USA 93, ... Morishita F, Nakanishi Y, Kaku S, Furukawa Y, OhtaS, Hirata T, Ohtani M, Fujisawa Y, Muneoka Y &Matsushima O (1997) A novel D -amino- acid- containingpeptide isolated from Aplysia heart. Biochem ... T,Watanabe K, Funase K, Sun XP, Yongsiri A, KimKH, Novales-Li P et al. (1989) Achatin-I, an endo-genous neuroexcitatory tetrapeptide from Achatinafulica Fe´russac containing a D -amino acid...

Báo cáo khoa học: Changes in speciﬁc lipids regulate BAX-induced mitochondrial permeability transition pptx

... conformational changes [1–4], becoming a mediator of the intrinsic phase of apoptosis by itsinsertion into mitochondrial membranes, a process thatculminates in the release of cytochrome c and activa-tion ... and the incision was closed, simulating thyroidec-tomy.Puriﬁcation of BAX-DC (rBAX) proteinRecombinant GST–BAX-DC (protein lacking the C-termi-nal 20 amino acids) was prepared according to ... and western blotting. The horizontal line indicates the samples with added rBAX. Membranes were incubated againstanti-BAX mAb, stripped, and evaluated for VDAC content to check protein loading....

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