... Miyazawa K, Tsubouchi H, Naka D, Takahashi K,Okigaki M, Arakaki N, Nakayama H, Hirono S, Sakiy-ama O, Takahashi K et al. (1989) Molecular cloning and sequence analysis of cDNA for human hepatocytegrowth ... Coomassie Brilliant Blue. The intensity of the band of the protease domain was quantified using bovine serum albu-min (BSA) as a standard. The enterokinase-treated pro-TMPRSS13 obtained fromCOS-7 ... of the 67 kDa band suggests that the pro -protein wascleaved at a single site, and the cleaved protein is a two-chain form linked by a disulfide bond. The prote-ase domain of TMPRSS13 was quantified...