Báo cáo khoa học: Odorant binding protein has the biochemical properties of a scavenger for 4-hydroxy-2-nonenal in mammalian nasal mucosa doc

... 2006 FEBS Odorant binding protein has the biochemical properties of a scavenger for 4-hydroxy-2-nonenal in mammalian nasal mucosa Stefano Grolli, Elisa Merli, Virna Conti, Erika Scaltriti and Roberto ... the 16-h incubations, in which at least 50% of residual lig-and -binding capacity was maintained at the highest[HNE] ⁄ [OBP] values. In the case of 20-min incuba-tions, HNE inactivation was even ... HNE andAMA are shown for porcine (C) and bovine (D) OBP. Protein samples were incubated with a ﬁxed saturating amount of AMA and increasingHNE. Each point on the y-axis shows the concentration...

Báo cáo khoa học: Evolutionary changes to transthyretin: structure and function of a transthyretin-like ancestral protein doc

... TLP(5-HIUase) and OHCU decarboxylase] involved in the oxidation of uric acid to allantoin. Certainly, the co-localization of these proteins in the peroxisomes of metazoan and plant species, and the ... on the surface of mucosal epithelial tissues of all animals as part of the innate immune system [47] and is also thought to actas a microbicidal agent in these instances. Because uricacid can ... hominoids lost their ability todegrade uric acid as the result of the inactivation of the uricase gene in a primate ancestor, some 15 Ma[42]. In humans, the TLP gene has several inactivatingmutations...

Báo cáo khoa học: Antioxidant Dps protein from the thermophilic cyanobacterium Thermosynechococcus elongatus An intrinsically stable cage-like structure endowed with enhanced stability potx

... provided by T. Kaneko, KazusaDNA Research Institute), using primers Dps-Te1 (5¢-CAAAGGAGACTCATATGAGTGCAACAACTAC-3¢) andDps-Te2 (5¢-CTACAAAAGCTTAATCCGCAACTAACTGAC-3¢). The NdeI and Hin dIII restriction ... does not warrant the calculation of thermodynamic parameters.DNA -binding ability and DNA protection againsthydroxyl radicals Binding of Dps-Te to DNA was analysed in vitro bymeans of agarose gel ... deposited in the Protein Data Bank (Accession number 2C41).DNA -binding and protection assays The DNA -binding ability of Dps-Te was assessed in gel-shift assays using supercoiled pET-1 1a DNA (5600...

Báo cáo khoa học: MAP2 prevents protein aggregation and facilitates reactivation of unfolded enzymes ppt

... MAPs are again a mixture of proteins containing predominantly MAP2 and tau withtraces of MAP1, MAP4, etc. [33]. We checked the Fig. 1. Insulin and ADH aggregation in the presence of MTP. (A) Aggregation ... segregated acidic amino acids sequence(53–58, 104–107) are found in the N-terminal sequence of tau. In contrast, the N-terminal domain of MAP2 containsabout 26 patches of acidic amino acids (Table ... From the sequence analysis it is apparent that the presence andabsence of acidic amino acid patches might also modulate the chaperone-like activity of the proteins. As all theseMAPs have similar...

Tài liệu Báo cáo khoa học: Branched N-glycans regulate the biological functions of integrins and cadherins doc

... has a large extracellularregion, a single transmembrane domain, and a shortcytoplasmic tail (except for b4). The N-terminaldomains of the a- and b-subunits associate to form the integrin ... ﬁndings mayexplain why bisecting GlcNAc-containing N-glycansare abundant in the brain [65]. In fact, mice carryingan inactive GnT-III mutant have an atypical neurolog-ical phenotype [66]. The ... Noda K, Higashiyama S, IharaH, Matsuura N, Hayashi N, Kawata S, Matsuzawa Y& Taniguchi N (2001) The addition of bisecting N-acet-ylglucosamine residues to E-cadherin down-regulatesthe...

Tài liệu Báo cáo khoa học: Rotary F1-ATPase Is the C-terminus of subunit c ﬁxed or mobile? docx

... the coiled-coil region of c and the complete a- helical C-terminusas held within the top of (ab)3. The rotary axis z was alignedalong the main axis of the shaft. The ‘bearing’ included a total of 138 residues ... 2).Molecular dynamics simulations of the rotary mobility of c within the ‘hydrophobic bearing’ at the top of a 3b3 The molecular model of the rotary part of c and the surrounding part of (ab)3was ... 2004understandable, because the samples were diluted during the activity a ssay and a dded ATP displaced the residualamounts of AMP-PNP and ADP from the catalytic sites of the enzyme. Activity measurements...

Tài liệu Báo cáo khoa học: Unfolding and aggregation during the thermal denaturation of streptokinase pptx

... to the sum of a number of two-state transitions. In these fittings the heat capacity change, DCp,fortheunfolding of each domain was fixed by using the valuesobtained from the analysis of the ... DCpUwerefixed in the fits using the values in Table 1 for SKA atpH 7.0. In addition, for the sake of simplicity the relativeheat capacity function of the aggregate, DCp ,A ,andtheactivation enthalpy for ... temperature provides the heat capacity relative to N, also known as the excessheat capacity. The addition of the heat capacity of the native state, Cp(N), gives the total heat capacity of the protein: Cp¼...

Báo cáo khoa học: Human telomeric G-quadruplex: The current status of telomeric G-quadruplexes as therapeutic targets in human cancer pdf

... anticancer activity in tumour xenograft models, which indicatethat the approach may be applicable to the treatment of a wide range of human cancers. This minireview summarizes the available data on thesecompounds ... p16INK 4a kinase and p53 pathways [32–35]which can be visualized by the appearance of charac-teristic DNA damage foci using an antibody to the damage response protein cH2AX [36], or by a signiﬁ-cant ... number of assays. The mostimportant are: (a) high-afﬁnity in vitro telomeric quad-ruplex binding, with a K a value of at least 106m)1; (b) a low level of binding to duplex DNA, with a K a valueat...

Báo cáo khoa học: Identiﬁcation, subcellular localization and functional interactions of PilMNOWQ and PilA4 involved in transformation competency and pilus biogenesis in the thermophilic bacterium Thermus thermophilus HB27 ppt

... essential for transformation and may be implicated in retraction of the PilA4-comprising DNA translocator transporting the DNA through the periplasmic space. Binding of the DNA to the DNA -binding protein ComEA ... membrane proteins that probably form part of the assembly platform and are involved in the assembly of the DNA translocatorcomplex in the inner membrane. The potential trafﬁc NTPase PilF is essential ... is involved in the stabilization of PilQ andPilA4 proteins in the outer membrane. Moreover, the absence of any PilW-like proteins in the transforma-tion machineries of mesophilic bacteria,...

Báo cáo khoa học: Trigger factor interacts with the signal peptide of nascent Tat substrates but does not play a critical role in Tat-mediated export pptx

... pTorA/P2 [ 17] a s a template and the primersRRTorA-SacI-fw (5¢-GCGCGGAGCTCAAGAAGGAAGAAAAATAATGAAC-3¢, SacI site underlined) andTorA/Lep2-BamHI-rv (5¢-GCATGGATCCCGCGCGCTTGATGTAATC-3¢, BamHI site ... speciﬁcity for the Tat machineryconsisting of the i ntegral membrane proteins TatA/E, TatBand TatC [11].Little is known about the molecular mechanism of targeting and export of Tat-dependent proteins. ... translocation (Tat)-mediated protein trans-port across the bacterial cytoplasmic membrane occurs onlyafter synthesis and folding of the substrate protein thatcontains a signal peptide with a characteristic...

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