... structures. At 10 min,punctate staining diminished and vacuolar staining in- creased, and finally punctate staining was almost lostwith predominant vacuolar staining at 30 min. Com-pared with ... of Medicine, National University of Singapore, Republic of Singapore2 Institute of Molecular and Cell Biology, A*STAR Biomedical Research Institutes, Singapore, Republic of Singapore3 Institute ... phosphatidylinositolphosphates (PtdInsP) including PtdIns(4)P, PtdIns(5)P,PtdIns(3,4)P2 and PtdIns(3,5)P2with the strongestbinding to PtdIns(5)P (Fig. 1). The ORD domainshowed a very similar lipid binding pattern as...