Báo cáo khoa học: NovelN,N¢-diacyl-1,3-diaminopropyl-2-carbamoyl bivalent cationic lipids for gene delivery – synthesis,in vitro transfection activity, and physicochemical characterization docx

... ¢-diacyl-1,3-diaminopropyl-2-carbamoyl bivalent cationic lipids for gene delivery – synthesis, in vitro transfection activity, and physicochemical characterization Michael Spelios and Michalakis SavvaDivision of Pharmaceutical ... PL(1994) Enhanced gene delivery and mechanism studieswith a novel series of cationic lipid formulations. J BiolChem 269, 255 0–2 561.M. Spelios and M. Savva Novel cytofectins for gene delivery FEBS ... Design,synthesis, and in vitro gene delivery efﬁcacies of novelcholesterol-based gemini cationic lipids and their serumcompatibility: a structure–activity investigation. J MedChem 50, 243 2–2 442.5...

Tài liệu Báo cáo khoa học: Angiopoietin-like proteins: emerging targets for treatment of obesity and related metabolic diseases pptx

... ANGPTL2 and ANGPTL6 ⁄ AGF in obesity and related metabolic diseases, and discuss the possibility that both could func-tion as molecular targets for the prevention and treatment of obesity and metabolic ... focus onthe roles of ANGPTL2 and ANGPTL6 ⁄ AGF in obes-ity and related metabolic diseases, and discuss whetherthese ANGPTLs could be targets for the prevention and treatment of these conditions.Suppression ... indicating thatit is secreted [9,13]. ANGPTL6 ⁄ AGF induces angio-genesis and arteriogenesis through activation of theERK1 ⁄ 2–eNOS–NO pathway in endothelial cells[6,9,16,23].ANGPTL6 ⁄ AGF null...

Tài liệu Báo cáo khoa học: A novel electron transport system for thermostable CYP175A1 from Thermus thermophilus HB27 doc

... The Kmvalue for NADH was determined in the pre-sence of FNR (200 nM) and NADH (0. 5–7 .0 mM), and the Kmvalue for NADPH was determined in the presence of FNR (20 nM) and NADPH ( 2–1 00 lM).NADH ... vector, and theconstruct was designated pET–FNR. E. coli BL21(DE3)Codon Plus cells were transformed with pET–FNR. Thetransformant was grown in 2 · YT medium containingchloramphenicol and ampicillin ... via OFOR and Fdx to CYP119 [13,14]. Inter-estingly, the electron transport system for CYP175A1did not utilize OFOR, although the T. thermophilusHB27 genome contains the genes encoding OFOR(TTC1591...

Tài liệu Báo cáo khoa học: Biosynthesis of riboﬂavin Screening for an improved GTP cyclohydrolase II mutant pdf

... template for a second cycle of mutagenesis and selection (10 000 mutants). It afforded 351 novelcandidate strains. After rescreening, 10 mutant proteinswere purified and characterized, and their ... weresupplied by Fluka (Buchs, Switzerland). LB (Luria–Bertani)medium was from Becton Dickinson (Basel, Switzerland).Generation of mutant libraries For the generation of the ribA mutant library, ... protein domain wassubjected to in vitro mutagenesis by error-prone PCR(on average, two to five mutations per gene) , and theresulting amplificates were ligated to the gene segmentspecifying the 3,4-dihydroxy-2-butanone...

Tài liệu Báo cáo khoa học: Helicases ) feasible antimalarial drug target for Plasmodium falciparum pptx

... attributed to mutations inhelicase genes [ 1–4 ]. Helicases act on double-strandednucleic acid substrate and thus can be designatedDNA–DNA, RNA–DNA or RNA–RNA helicasesdepending on the composition ... beensuggested for a number of the conserved motifs [1 0– 12]. For example, motif I (A ⁄ GxxGxGKT), motif II(VLDEAD), motif III (SAT) and motif VI(HRIGRxxR) are responsible for ATP binding and hydrolysis, ... molecular tools for cel-lular machinery and signiﬁcantly contribute to normalcellular metabolism. In general, helicases require a sin-gle-stranded nucleic acid region to bind and start theiraction...

Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-speciﬁc blood vascular and lymphatic endothelial cells of the mouse pdf

... 5¢-TGAAGGCAAATCTCTGGAC-3¢ for the former, and LTA–M2F, 5¢-CAGCTGTTTTGCTTGAATTATG-3¢ and LTA–2R, 5¢-GAATTCATTATGTTTCAGGTTCAGGGG-3¢ for the latter. The PCR products were cloned intothe EcoRV site of pZErO-2 (Invitrogen). ... 200 lm. (B) Prox–1 and VEGFR–3 were also expressed inLyve–1-positive ECs, conﬁrming that they maintained lymphaticendothelial properties. (C) VEGF–A and VEGF–C induced MAPK and Akt activation ... ofangiogenesis and lymphangiogenesis. Nat Rev Mol CellBiol 8, 46 4–4 78.2 Aird WC (2007) Phenotypic heterogeneity of the endo-thelium: I. Structure, function, and mechanisms. CircRes 100, 15 8–1 73.3...

Tài liệu Báo cáo khoa học: Phage-display as a tool for quantifying protein stability determinants pptx

... bonded pairs have different Ca and Cbdistances than hydrogen bonded pairs and therefore mayhave different residue preferences and potential for sidechain–side chain interactions. Initial ... varied (for instance, coreresidues 3 and 39, and boundary residues 25 and 29 thatwere all changed in a hyperstable GB1 variant [12]). Basedon the computational studies, core positions 5 and 30 ... cross-strand residues, 44 and 53 (Fig. 5). Thesepositions were guest sites in published mutagenesis studiesfrom which b-sheet propensity scales have been developed[2 1–2 4]. In both protein mutagenesis...

Tài liệu Báo cáo khoa học: "Unsupervized Word Segmentation: the case for Mandarin Chinese" doc

... Association for Computational Linguistics, pages 38 3–3 87,Jeju, Republic of Korea, 8-14 July 2012.c2012 Association for Computational LinguisticsUnsupervized Word Segmentation:the case for Mandarin ... II (Emerson, 2005) and deﬁne a more precise topline for the taskusing cross-trained supervized system availableoﬀ-the-shelf (Zhang and Clark, 2010; Zhao and Kit, 2008; Huang and Zhao, 2007)1 ... and 2, Wang etal. (2011) try not to rely on punctuation and char-acter encoding information (such as distinguishingLatin and Chinese characters). However, they opti-mize their parameter for...

Tài liệu Báo cáo khoa học: Stem–loop oligonucleotides as tools for labelling double-stranded DNA pdf

... 77 1–7 77.Stem–loop padlock oligonucleotides for dsDNA B. Ge´ron-Landre et al.5352 FEBS Journal 272 (2005) 534 3–5 352 ª 2005 FEBSStem–loop oligonucleotides as tools for labellingdouble-stranded ... TÆAxT and CÆGxG triplets with an antiparallelTG TFO.Stem–loop padlock oligonucleotides for dsDNA B. Ge´ron-Landre et al.5344 FEBS Journal 272 (2005) 534 3–5 352 ª 2005 FEBSTFO and can form ... theTFO forms a triplex with the target dsDNA. The 5¢ -and3 ¢-part ofthe TFO hybridize to each other, thereby forming a short double-stranded stem, and leaving a four nucleotide single-stranded...

Tài liệu Báo cáo khoa học: "Automatically Extracting Polarity-Bearing Topics for Cross-Domain Sentiment Classiﬁcation" pptx

... thein-domain gold standard classiﬁcation result. For example, the in-domain goal standard for the Bookdomain is 79.96%. For adapting from DVD to Book,baseline achieves 72.25% and JST gives 76.45%.The ... 257859).ReferencesR.K. Ando and T. Zhang. 2005. A framework for learn-ing predictive structures from multiple tasks and un-labeled data. The Journal of Machine Learning Re-search, 6:181 7–1 853.A. Aue and M. ... lizidenotes a combination ofsentiment label li and topic zi for word wi. Finally,feature selection is performed according to the infor-mation gain criteria and a classiﬁer is then trainedfrom the...

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