Báo cáo khoa học: Human retinol dehydrogenase 13 (RDH13) is a mitochondrial short-chain dehydrogenase⁄reductase with a retinaldehyde reductase activity pdf

... 138 –147 ª 2007 The Authors Journal compilation ª 2007 FEBS 147 Human retinol dehydrogenase 13 (RDH13) is a mitochondrial short-chain dehydrogenase ⁄ reductase with a retinaldehyde reductase activity Olga ... 4). Activity assays showed that puriﬁed RDH13–His6 wasindeed active towards all-trans -retinaldehyde andappeared to prefer NADPH to NADH as a cofactor,because the conversion of 5 lm all-trans -retinaldehyde in ... puriﬁed RDH13–His6 wasraised at Alpha Diagnostics International Inc. (San Antonio,TX, USA).For western blot analysis of RDH13 expression, samplesof human tissue obtained from the Anatomical Gift...

Báo cáo khoa học: Crystal structure of the second PDZ domain of SAP97 in complex with a GluR-A C-terminal peptide ppt

... Molecular GraphicsSystem. DeLano Scientiﬁc, San Carlos, CA, USA.41 Wallace AC, Laskowski RA & Thornton JM (1995)LIGPLOT: a program to generate schematic diagramsof protein–ligand interactions. ... GluR -A and of twovariant PDZ2 domains in unliganded state at 1.8–2.44 A ˚resolutions.SAP97PDZ2folds to a compact globular domain comprising six b-strandsand two a- helices, a typical architecture ... consistent with a ‘classical’ type I PDZ interaction between SAP97and GluR -A C-terminus.AbbreviationsAMPA, a- amino-5-methyl-3-hydroxy-4-isoxazole propionic acid; GluR -A, ionotropic glutamate...

Tài liệu Báo cáo khoa học: Human Cdc45 is a proliferation-associated antigen pdf

... maintenance, and is essential for chromo-somal DNA replication. Proc Natl Acad Sci USA 93,12309–12314. 13 Mimura S, Masuda T, Matsui T & Takisawa H (2000)Central role for cdc45 in establishing ... antibody on formalin-ﬁxed andparafﬁn-embedded tissues (Fig. 9). Antibodies againstPCNA and Cdc45 stained malignant cells in a compar-able manner, e.g. on invasive-lobular mamma carci-noma sections ... in histological material is a valuable component of conventional histopathologi-cal analysis and may be of major prognostic import-ance [56]. Proliferation in immunohistochemicalsections can...

Tài liệu Báo cáo khoa học: Human enhancer of rudimentary is a molecular partner of PDIP46/SKAR, a protein interacting with DNA polymerase d and S6K1 and regulating cell growth docx

... GCGGGATCCAACAAGGAAGAACCCCCCATAAGAATGCGGCCGCTCAGTCTGAGGTGATAACATTCCCpYESTrp2 ⁄ PDIP46⁄ SKAR(F) GCGGGATCCCTGGACGGGCAGCCGATGAAGATAAGAATGCGGCCGCTCAAAGCTTGATTTTGAATTCTGTpYESTrp2 ⁄ PDIP46 ⁄ SKAR(G) ... SKAR(b) AAACTGCAGGATGGCGGACATCTCCCTGGACAAACTGCAGAAGCTTGATTTTGAATTCTGTpEGFP-N1 ⁄ MEK1 GCGAAGCTTCACGATGCCCAAGAAGAAGCCGACGCCGCGGGATCCCGGATGCTGGCAGCGTGGGTTGGpYESTrp2 ⁄ PDIP46 ⁄ SKAR (A) GCGGGATCCAACAAGGAAGAACCCCCCATAAGAATGCGGCCGCTCAAGGCAGCTCGCTCTCCTTTTTpYESTrp2 ... GCGGGATCCAACAAGGAAGAACCCCCCATAAGAATGCGGCCGCTCAAAGCTTGATTTTGAATTCTGpEGFP-N1 ⁄ ER GCGAAGCTTCACGATGTCTCACACCATTTGCGGGATCCCGTTTCCCAGCCTGTTGGGCCTpEGFP-N1 ⁄ PDIP46(1) ⁄ SKAR (a) AAACTGCAGGATGGCGGACATCTCCCTGGACAAACTGCAGAAGCTTGATTTTGAATTCTGTpEGFP-N1...

Tài liệu Báo cáo khoa học: The thioredoxin-independent isoform of chloroplastic glyceraldehyde-3-phosphate dehydrogenase is selectively regulated by glutathionylation docx

... QuickChange site-directed mutagenesis kit(Stratagene, La Jolla, CA, USA): C153S-up, 5¢-TGCATCTTGCACTACCAACTCTCTTGCTCCCTTTGTC-3¢; andC153S-down, 5¢-TGACAAAGGGAG CAAGA GAGTTGGTAGTGCAAGATGC-3¢. ... triangles). NADPH-dependent activity is given as a percentage of the initial activity. (B) Reversal of A 4-GAPDH inactiva-tion by dithiothreitol. A 4-GAPDH was inactivated by incubation with 0.1 ... Activity is given as a percentage of the initial activity. Note that the speciﬁc activity of A 8B8-GAPDH with NADPH as the coenzyme is about fourfold lowerthan that of A 2B2-GAPDH. (C) Reversal...

Tài liệu Báo cáo khoa học: Transient silencing of Plasmodium falciparum bifunctional glucose-6-phosphate dehydrogenase) 6-phosphogluconolactonase docx

... (PF08–0071) CAACGCTGCTCAAATATGGA CATGAGGCTCACCACCACA CGCGCCTACTTTTTACTGGGATTCTATGGGACCTGGCGCGPfG6PD-6PGL (X74988) GAACTCCAGGAAAAACAAGTCAAG TTTTGACAAGTCCAAATACCTCTTT CGGCCAACGTTAAAAAGTATCGGATGGAATTTTGGCCGPfGPx ... AGTGGAGGAATGGCTGCAG CCTAAACGGGATTTTTCGACA CGGGCAGCAAGGCATAACGCAAGCCCGPfTrxR (AL929357) TTGTACTAATATTCCTTCAATATTTGCTG GCCACGGGCGCTAATT CCGGGCTGTAGGAGACGTAGCTGAAAATGTCCCGGPfSOD (PF08–0071) CAACGCTGCTCAAATATGGA ... (PFL0595C) AATTGTGATTCGATGCATGATG TTTATCGACGAGAAATTTTCCAA CGGCCAACGTTAAAAAGTATCGGATGGAATTTTGGCCGTransient silencing of G6PD-6PGL A. Crooke et al.1544 FEBS Journal 273 (2006) 1537–1546 ª 2006 The Authors...

Tài liệu Báo cáo khoa học: Electron transfer chain reaction of the extracellular ﬂavocytochrome cellobiose dehydrogenase from the basidiomycete Phanerochaete chrysosporium doc

... Journal 272 (2005) 2869–2877 ª 2005 FEBS 28755¢-TCAGCGTTCTCGGAATTC-3¢; AP2-Xba-R, 5¢-TTTTACAGTAATATAAAGAATTTCGCTCTAGATCAAGGACCTCCCGCAAGCGCGAG-3¢; and AP2-R, 5¢-TTACAGTAATATAAAGAATTTCGCTCTAGA-3¢, ... MgCl2using an ALSElectrochemical Analyzer 62 4A, and the potential was deter-mined by averaging the anodic and cathodic peak potentials.Presteady-state kinetic studiesPresteady-state reduction of ﬂavin ... vectorpPICZa -A ⁄ f166y was then linearized with Bpu1102I(TaKaRa Bio) and transformed into Pichia pastoris KM-71H using a MicroPulser electroporation device (Bio-RadLaboratories, Hercules, CA, USA)....

Tài liệu Báo cáo khoa học: Human intrinsic factor expressed in the plant Arabidopsis thaliana doc

... potentialas a large-scale source of human IF for analytical andtherapeutic purposes.Keywords: arabidopsis; cobalamin; intrinsic factor; recom-binant.Vitamin B12(cobalamin, Cbl) is the ... signal peptide (Ext) with the amino acid sequence MASSSIALFLALNLLFFTTISA and 47 nucleotides from the 5¢-untranslatedregion. This sequence is part of the plant A. thalianacDNA sequence in GenBank ... to be an excellent source of IFfor analytical application and, possibly, for therapeuticdevelopment.Materials and methodsPreparation of the genetic material A cDNA for human IF was prepared...

Tài liệu Báo cáo khoa học: "Human Evaluation of a German Surface Realisation Ranker" docx

... Hill RoadPalo Alto, CA 94304, USAmforst@parc.comAbstractIn this paper we present a human- basedevaluation of surface realisation alterna-tives. We examine the relative rankings ofnaturally ... European Chapter of the ACL, pages 112–120,Athens, Greece, 30 March – 3 April 2009.c2009 Association for Computational Linguistics Human Evaluation of a German Surface Realisation RankerAoife ... string accuracy, gener-ation tree accuracy, word accuracy (Bangalore etal., 2000; Callaway, 2003; Nakanishi et al., 2005;Velldal and Oepen, 2006; Belz and Reiter, 2006).It is not always clear...

Báo cáo khoa học: Human kynurenine aminotransferase II – reactivity with substrates and inhibitors potx

... transamination.AbbreviationsAAD, a- aminoadipate; AlaAT, alanine aminotransferase; AspAT, aspartate aminotransferase; BCA, b-chloroalanine; CNS, central nervoussystem; CSA, cysteine sulﬁnate; ESBA, (R)-2-amino-4-(4-(ethylsulfonyl))-4-oxobutanoic ... S, Cartiaux M & Urrestarazu A (1998)Characterisation of Saccharomyces cerevisiae ARO8and ARO9 genes encoding aromatic aminotransferas-es I and II reveals a new aminotransferase subfamily.Mol ... kynurenine aminotransferase II – reactivity with substrates and inhibitorsElisabetta Passera1, Barbara Campanini1, Franca Rossi2, Valentina Casazza2, Menico Rizzi2, RobertoPellicciari3and...

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