Báo cáo khoa học: Liver receptor homolog-1 localization in the nuclear body is regulated by sumoylation and cAMP signaling in rat granulosa cells docx

... ª 2008 The Authors Journal compilation ª 2008 FEBS Liver receptor homolog-1 localization in the nuclear body is regulated by sumoylation and cAMP signaling in rat granulosa cells Feng-Ming Yang, ... 2008)doi:10.1111/j.1742-4658.2008.06785.x Liver receptor homolog-1 (LRH-1; NR5A2) is an orphan member of the nuclear receptor superfamily, mainly expressed in endoderm-derived tissues and in the ovary. In ovarian granulosa and luteal ... activity [32]. There-fore, we tested whether cAMP signaling could affect the subnuclear localization of LRH-1 in granulosa cells. The primary rat granulosa cells cotransfectedwith LRH-1 and SUMO-1...

Tài liệu Báo cáo khoa học: A systems biology approach for the analysis of carbohydrate dynamics during acclimation to low temperature in Arabidopsis thaliana doc

... depends on the concentration of the reaction product and the dissociation constant Ki,Bforinhibitor binding. The rate of hexose-based sucrose synthesis was described by the mass action rate law:rA!BðtÞ¼k ... perhour. Data points were spline-interpolated to obtain time-continuous information on net photosynthesis during the whole period of cold exposure. The rate of raffinose synthe-sis, rSuc fi Raf, ... Á cAðtÞ In this reaction kinetic, the reaction rate rAfiB(t) dependson the substrate concentration cA(t) and the rate constant k. The model code and a detailed description of the modelstructure...

Tài liệu Báo cáo khoa học: Neuropeptide Y-family receptors Y6and Y7in chicken Cloning, pharmacological characterization, tissue distribution and conserved synteny with human chromosome region docx

... Y6 in brain. (B) Actin in brain. (C) Y6 in peripheraltissues. (D) Actin in peripheral tissues. The negative control sample included water in- stead of cDNA. The brain regions are named in accordance ... Y6gene is contained within one exon and encodes a protein of 374 amino acids displaying the characteristics of other NPY family receptors (Fig. 1),including two well-conserved cysteines presumed ... analysis suggests that the replacement rate for Y6was lower earlier in evolution and that the rate has increased in the mammalian lin-eage (Fig. 2) [41]. This, together with the fact that the gene...

Báo cáo khoa học: DNA polymerase e associates with the elongating form of RNA polymerase II and nascent transcripts pot

... blocking was controlled by performing the labelling procedure in the absence of the second primary antibody. The sections were embedded in methylcellulose and examined in a Philips CM100 transmis-sion ... Picontaining 5% BSA and 0.1% gelatine. Antibodies and gold conjugates were diluted in NaCl ⁄ Picontaining 0.1%BSA-C (Aurion, Wageningen, the Netherlands). All wash-ing steps were performed in ... Finland) of propidium iod-ide-stained cells [65] in parallel cell cultures. Where indi-cated, cells were cultured in the presence of the indicatedconcentrations of one of the following inhibitors:20...

Báo cáo khoa học: Akt-dependent phosphorylation negatively regulates the transcriptional activity of dHAND by inhibiting the DNA binding activity pdf

... examinewhether the DNA binding activity of dHAND could beaffected by Akt phosphorylation. The in uence of phos-phorylation by Akt on the DNA binding activity wasexamined by gel shift analysis. ... cAMP- dependent protein kinase, protein kinase C and caseinkinase II [36–42]. The activation of cAMP- dependentprotein kinase or protein kinase C can inhibit muscledifferentiation, whereas casein kinase II ... may explain apart of this involvement b ecause there is the report that Akt is expressed in developing heart [49]. Interestingly, the sequence of phosphorylation sites in the bHLH domain is well...

Báo cáo khoa học: Recruitment of coregulator complexes to the b-globin gene locus by TFII-I and upstream stimulatory factor doc

... to the b-globin genepromoter through the interaction with other DNAbinding proteins involved in repressing the adult glo-bin gene. One possible candidate is BP1, a homeo-domain-containing ... to the b-globin genepromoter in K562 cells. In the present study, we ana-lyzed the interaction between TFII-I and HDAC3 in both MEL and K562 cells and show that this inter-action is restricted ... analyzed interactions between USF and p300 in MEL and K562 cells by co-immunoprecipitation(Fig. 1D). The results demonstrate that USF interactswith p300 in MEL cells but not in K562 cells. ThisCoregulators...

Báo cáo khoa học: Lipid-induced conformational transition of the amyloid core fragment Ab(28–35) and its A30G and A30I mutants docx

... self-association and to measure distanceswithin proteins and other macromolecules [35]. In order to understand the folding and unfolding ofpeptides, both in the presence and the absence of PGlipids, ... maybe due to internal quenching by the Lys next to the Trp [33].Acrylamide quenching studiesA prerequisite for any understanding of the interactionof the peptide with the membrane is the knowledge ... ð2Þwhere Io and I are the ﬂuorescence intensities in the absence and presence of the quencher, respectively, Ksv is the dynamic quenching constant, and [Q] is the quencher con-centration. The Ksvvalue...

Báo cáo khoa học: Targeted disruption of one of the importin a family members leads to female functional incompetence in delivery docx

... signiﬁcantlydownregulated in imp a5) ⁄ )mice, by  20%. Theseﬁndings indicate that importin a5 is prominentlyinvolved in gene regulation by ER and its cofactors.On the other hand, the protein levels in ... upregulated in the brain,suggesting that the counter-regulation of another im-portin a isoform may compensate for the lack of asingle isoform in vivo in mammals [21]. Therefore, todetermine ... import. On the otherhand, the importin a family generally binds to both the nuclear import cargo and importin b, indicatingthat importin a functions as an adaptor between the cargo proteins and importin...

Tài liệu Báo cáo khoa học: Liver X receptor agonists inhibit tissue factor expression in macrophages docx

... collected and then DNA-binding activityof NFjB p50 and p65 was assessed. There were no sig-niﬁcant changes exhibited in the protein–DNA com-plexes induced by LPS in the presence of LXRagonists ... coactivator-1(SRC-1) and activating signaling cointegrator-2 [34–39].Another possible mechanism is that a direct protein–protein interaction between LXR and NFjBorbetween LXR and another transcription ... nuclear factor-jB (NFjB), Sp1 and early growth response(Egr)-1 binding sites [16]. Liver X receptors (LXRs), LXRa and LXRb, aremembers of the nuclear receptor superfamily and areinvolved in...

Tài liệu Báo cáo khoa học: EGF receptor in relation to tumor development: molecular basis of responsiveness of cancer cells to EGFR-targeting tyrosine kinase inhibitors docx

... JapanIntroduction The epidermal growth factor receptor (EGFR) is com-posed of an extracellular ligand-binding domain, atransmembrane domain and an intracellular tyrosinekinase domain. The binding ... of the cell-cycle by phos-phorylating the retinoblastoma (RB) protein. Activa-tion of cyclin–CDK complexes is counterbalanced by CDK inhibitors, including those of the kinase inhibitorproteins ... p15INK4bareresistant to the growth-inhibitory effects of geﬁtinib.As the level of p15INK4b is increased by MEK inhibi-tors, but not by Akt inhibitors, the induction ofFig. 3. The cell-cycle is arrested...

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