Báo cáo khoa học: Atomic-resolution structure of reduced cyanobacterial cytochrome c6 with an unusual sequence insertion pot
Báo cáo khoa học: Atomic-resolution structure of reduced cyanobacterial cytochrome c6 with an unusual sequence insertion pot
... FEBS Atomic-resolution structure of reduced cyanobacterial cytochrome c6 with an unusual sequence insertion Wojciech Bialek1, Szymon Krzywda2, Mariusz Jaskolski2,3and Andrzej Szczepaniak11 ... determination of thecrystal structure of cytochrome c6 and comparison with plastocyanin. Structure 3, 1159–1169.15 Kerfeld CA, Anwar HP, Interrante R, Merchant S &Yeates TO (1995) The structure of ... understanding of themolecular determinants of the spectroscopic andelectron transfer properties of this class of proteins.Results and Discussion Structure descriptionOverall, the structure of...
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Tài liệu Báo cáo khoa học: NMR structure of AII in solution compared with the X-ray structure of AII bound to the mAb Fab131 pptx
... (dark blue colour) and (B) structure alignment of the fragment 4–7 of the 13 ensemble structures of AII to the fragment 3–6 of the X-ray structure of AII.Fig. 6. Structure of a representative ... small rearrangements of the epitope4–7 in the solution structure of AII are required by a meanvalue of 0.76 ± 0.03 A˚for structure alignment and 1.27 ± 0.02 A˚for sequence alignment with the ... fairlycompact folded structure, with its C-terminus and N-ter-minus approaching to within 7.2 A˚ of each other. The sidechains of Arg2, Tyr4, Ile5 and His6 are oriented on one side of a plane defined...
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Báo cáo khoa học: Crystal structure of a staphylokinase variant A model for reduced antigenicity pptx
... Crystal structure of a staphylokinase variantA model for reduced antigenicityYuhang Chen1,IGang Song2, Fan Jiang1, Liang Feng1, Xiaoxuan Zhang1, Yi Ding1, Mark Bartlam1,Ao Yang1, ... as a cofactor to form a 1 : 1 complex with humanplasmin(ogen). The SAK±plasmin (cofactor±enzyme) com-plex, which has proteolytic activity, can form an enzyme±substrate c omplex with another ... K109A,K130T, K135R, K136A, and insertion K137) have much reduced polyclonal human antibody binding capacity whileretaining f ull ®brinolytic potency and ®brin-selectivity in ahuman plasma milieu [20]....
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Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt
... solution with four molecules in an asymmetric unit, with a Matthewscoefficient probability of 2.61 A3ÆDa)1and a solventcontent of 52.9%. Interestingly, molecules A and B(and C and D) formed ... Kruus2,Juha Rouvinen1and Nina Hakulinen11 Department of Chemistry, University of Eastern Finland, Joensuu, Finland2 VTT Technical Research Centre of Finland, Espoo, FinlandKeywordsascomycete; ... crystal structure of TaLcc1 does not have a chloride attached tothe T2 copper, as observed in the crystal structure of MaL. The unique fea-ture of TaLcc1 and MaL as compared with other laccases structures...
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Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt
... was transformed into BL21 (DE3) cells and grown at37 °C overnight in Luria–Bertani medium containing30 lgÆmL)1 of kanamycin. Flasks of 400 mL of Luria–Bertani medium (30 lgÆmL)1kanamycin) ... importin-a and the chloride intracellular chan-nel (CLIC) protein, CLIC4 [2,3].The structure of a soluble form of CLIC4 showsthat it adopts the canonical glutathione S-transferasefold with an N-terminal ... moleculesand peptide atoms) and rjis the standard deviation of Bfactors. The normalized B factors have a zero mean and unitvariance. All atoms that satisfy Bz‡ 4 are treated as outliersand...
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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... (Fig. 3A). Three of the ligands,OD2 of Asp165, NE2 of His79 and NE2 of His169,formed an equatorial plane. The other protein ligand,NE2 of His31, bound to the metal, in the company of a water oxygen, ... Suzuki5and Yasushi Kawata3,41 National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka, Japan2 Department of Food Science and Nutrition, Faculty of Human Life and Science, ... coordi-nated with six ligands: the five same ligands in theMn-reconstituted enzyme and an additional water oxy-gen, which, together with the OD2 of Asp165, theNE2 of His79 and the NE2 of His169,...
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Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc
... modeled with a reduced occupancy of 0.6–0.8 to account for the likelyeffect of radiation damage [33].In order to determine the structure of the complex of an inactive PhyK mutant with its substrate ... the structure analysis server stan [32]. This model has an Rwork of 0.180and Rfree of 0.206. As suggested by the electron density, theScatoms of all six cysteine side chains were modeled with ... crystal structures of the ligand-free PhyK at 1.7 A˚resolution and of a cata-lytically incompetent PhyK mutant that suggest amodel for substrate binding. Comparison with thestructures of the...
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Tài liệu Báo cáo khoa học: Solution structure of hirsutellin A – new insights into the active site and interacting interfaces of ribotoxins docx
... determined the structure of HtA by NMR methods. The structure consists of one a-helix, a helical turn and seven b-strands thatform an N-terminal hairpin and an anti-parallel b-sheet, with a characteris-tic ... common tertiary structure [4,13,14]. They fold into an a + b structure with a central five-stranded antiparallel b-sheet and an Keywordscytotoxic protein; NMR; ribonucleases;RNase T1; structure; ... agrees with the arrangement present inother ribotoxins, and is fully compatible with thedistance restraints discussed below.After seven cycles of NOE assignment and structure calculation by cyana⁄...
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Tài liệu Báo cáo khoa học: Down-regulation of reduced folate carrier may result in folate malabsorption across intestinal brush border membrane during experimental alcoholism docx
... Human reduced folate carrier: translation of basic biology tocancer etiology and therapy. Cancer Metastasis Rev 26,111–128.24 Qiu A, Jansen M, Sakaris A, Min SH, ChattopadhyayS, Tsai E, Sandoval ... activity of the membrane transport sys-tem were not caused by a general loss of intestinal architecture, and hencecan be attributed to the specific effect of ethanol ingestion on the folatetransport ... performed with the use of gene-specific primers corresponding to a sequence in theopen reading frame of rat RFC and b-actin (as an internal control); products of 489 and 588 bp for RFCand b-actin,...
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Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx
... showsexcellent stereochemistry, with 98.5% of all residues in themost favored and favored regions of the Ramachandranplot and r.m.s.d. values for bond and angle of 0.005 A˚and1.37 ° as shown in Table ... crystal structure of the complex of this protease with benzamidine.Results and DiscussionOverall structure The catalytic domain of DESC1 resembles an oblateellipsoid with diameters of 38 and 48 ... proteinase(TTSP), an expanding protein family with membersdifferentially expressed in several organs and certaintumors. To date, more than 30 mammalian members of this group have been identified and have,...
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