Báo cáo khoa học: Structural characterization of N-linked oligosaccharides of laminin from rat kidney: changes during diabetes and modulation by dietary ﬁber and butyric acid pdf

... compilation ª 2010 FEBS 155 Structural characterization of N-linked oligosaccharides of laminin from rat kidney: changes during diabetes and modulation by dietary fiber and butyric acid Adishesha Puneeth ... N-linked oligosaccharides of laminin during diabetes and the likelihood of dietary fiber and butyric acid in mod-ulating these changes. ResultsRats experimentally induced with diabetes using strep-tozotocin ... dietary fiber and butyric acid. Abbreviations2AA, 2-anthranilic acid; FFC, dietary fiber- fed control; FFD, dietary fiber- fed diabetic; FFD-500, dietary fiber- fed diabetic with 500 mg butyric acid; PGNase-F,...

Tài liệu Báo cáo khoa học: Structural characterization of Ca2+/CaM in complex with the phosphorylase kinase PhK5 peptide pdf

... 1 molar ratio and then analysed by gel ﬁltration chromatography. The peakswere concentrated using 3 lL of Strataclean protein bind-ing beads (Stratagene, Amsterdam, the Netherlands) and then ... consisting of four copies of foursubunits, a, b, c and d. The a and b subunits are regu-latory and are the sites of phosphorylation and meta-bolite binding and are also regulated by the binding of ... obtained for both vertebrate and nonvertebrates by usingsequence from 296 to 386 of PhKc from rabbit muscle. Identities are shown by the blue boxes with white text, while blue text and greenboxes indicate...

Báo cáo khoa học: Structural characterization of a novel branching pattern in the lipopolysaccharide from nontypeable Haemophilus inﬂuenzae pot

... and this was confirmed and detailed by NMR spectroscopy (seebelow). Characterization of OS fractions and LPS-OH by NMRThe1H NMR resonances of OS fractions and LPS-OHwere assigned by 1H–1H chemical ... (b-configur-ation) or between H-1 and H-2 (a-configuration). On thebasis of the chemical shift data and the large J2,3, J3,4 and Table 4. Structures of the Hep4-glycoforms of nontypeable Haemophilus ... HepHepb|Hex2-Hex-Hep-AnKdo-ol|Hep|HepaMajor isomer of the Hex3 glycoform (estimated from the intensity of the fragments in MS2experiments).bMinor isomer of the Hex3 glycoform (estimated from the intensity of the fragments...

Báo cáo khóa học: Structural characterization of the human Nogo-A functional domains Solution structure of Nogo-40, a Nogo-66 receptor antagonist enhancing injured spinal cord regeneration ppt

... ecloned and expressed the two functional regions of humanNogo-A and performed structural characterization by CDCorrespondence to J. Song, Department of Biochemistry, NationalUniversity of Singapore; ... been demonstrated toenhance CNS neuronal regeneration. (C) The amino acid se quence of the N-terminal 24 residues of Nogo-40.Fig. 2. Expression and puriﬁcation of Nogo-A(567–748) and Nogo-66. ... 2004 NMR characterization of the Nogo-A functional domains (Eur. J. Biochem. 271) 3515ResultsExpression and structural characterization of Nogo-A(567–748) and Nogo-66Nogo-A(567–748) and Nogo-66...

Báo cáo khoa học: Structural characterization of photosystem II complex from red alga Porphyridium cruentum retaining extrinsic subunits of the oxygen-evolving complex docx

... 2,5-dichloro-p-benzoquinone at a concentration of 500 lM and ferricya-nide at a concentration of 2.5 mM and illuminated withsaturating white light.Gel ﬁltration chromatographyGel ﬁltration chromatography ... and separated in a linear 0–1Msucrosegradient. A pigment ratio of separated chlorophyll-con taining bands isindicated on t he right. (B) SDS/PAGE analysis of the three sucrosegradient bands ... Cˇeske´Budeˇjovice;3Laboratory of Photosynthesis, Institute of Microbiology, Academy of Sciences of the Czech Republic, Trˇebonˇ;4Institute of Physical Biology, University of South Bohemia, Cˇeske´Budeˇjovice,Czech...

Báo cáo khoa học: Structural characterization of the lipopolysaccharide O-polysaccharide antigen produced by Flavobacterium columnare ATCC 43622 potx

... experiments. Interresidue NOEs were seen from H1B to H4C andtoitsownH2B,fromH1A toH2A and across the glycosidic bond to H3B,andalsofromH1C to H3C and H5C and across the ring to H4A.HMBCexperiment ... followed from H1B to H2B and H3B, and from the methyl resonance of H6B to H5B with no evidence of connectivities to anyproton signals at C4B. The presence of a C4 keto groupfunction and the ... phenol (1.2 L), and, after cooling (4 °C) and low-speed centrifugation, the separated water and phenollayers were collected by aspiration, and dialyzed againstrunning water until free from phenol....

Báo cáo khoa học: Structural characterization of L-glutamate oxidase from Streptomyces sp. X-119-6 pdf

... comparisons of the funnels of LGOX, LAAO, and PAO, and the residues composing two entrances of the funnel of LGOX.(A) Stereo view of funnels of LAAO, PAO, and LGOX. The surfaces of the enzyme ... primary structure of LGOX exhibitsapproximately 20% and 12% identity, respectively,with those of LAAO and PAO. The alignments of sequences and secondary structure elements of LGOX and LAAO are presented ... structure of mature LGOX usingmolecular replacement with a structurally homologous model of l-amino acid oxidase (LAAO) from snake venom: LGOX has a deeply buried activesite and two entrances from...

Báo cáo khoa học: Structural characterization of the large soluble oligomers of the GTPase effector domain of dynamin potx

... domain of dynamin [7].The above possibility of functional dissection of thedynamin protein into speciﬁc domains suggests that adetailed characterization of the intrinsic structural and dynamic ... smallpropensity for formation of short helices, transiently, and the chain as such is highly dynamic.Structure of the core of the oligomersBecause of the large size of the core of the oligomer,the ... signals from theinterior of the core and thus do not give any informa-tion on its structural details. Nevertheless, we didderive useful insights into the structural aspects of thecore of the...

Báo cáo khoa học: Structural basis for the recognition of complex-type biantennary oligosaccharides by Pterocarpus angolensis lectin ppt

... protein and ligand concentrations, DG and DH were calculated assuming 5% lower or higher con-centrations of protein and ligand (highly conservative esti-mates for the errors on these concentrations). ... FEBSResultsStructure of unliganded PAL and its complexwith mannoseA correct understanding of ligand binding not onlyrequires structural data on the protein-carbohydratecomplexes, but also knowledge of the ... of mono- and oligosaccharides, binding of deoxy sugar analogs, hydroxyethyl analogs and con-formationally constrained sugars, analysis of solventisotope effects, osmotic stress strategies and...

Tài liệu Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera pptx

... aniso-tropy of the free minifiber, ABis the anisotropy of the mini- fiber bound to the penton base, [LigB] is the concentration of the bound ligand (minifiber), and [Ligtot] is the total con-centration ... acid ‘minifiber’construct was used as a fiber protein mimetic. Previous structural studies of hAd2pb with a 22 amino acid peptide from the N-terminus of the fiber protein havemapped the fiber protein ... theN-terminus of the fiber protein [20]. Structural align-ments of the hAd2 ⁄ 12pb chimera with the hAd2pb fiber peptide bound and unbound structures reveal ahigh degree of overall conservation (Figs 3B and...

Xem thêm