Báo cáo khoa học: Peptides that bind the HIV-1 integrase and modulate its enzymatic activity – kinetic studies and mode of action pptx

... increased the Km of the IN–DNA interaction, and were thus inhibitory; (b) peptides that slightly increased the Km of the IN–DNA interaction, but in addition modiﬁed the Vmax and Kcatvalues of the ... 3¢-end processing and strand-transfer assays wereperformed to study the mode of action of these peptides. The effects of the various peptides on the interaction between IN and its substrate DNA werealso ... of action and their effects on the kinetic parameters of the 3¢-end processing and strand-transfer steps of the integration reaction. Our results obtained in the cur-rent study reveal that the...

Báo cáo khoa học: Functional similarities between the small heat shock proteins Mycobacterium tuberculosis HSP 16.3 and human aB-crystallin docx

... similar to that of aB-crystallin [28].MTB HSP 16.3 of M. tuberculosis may be ideally suitedfor studies of the structure and function of the coreÔa-crystallinÕ domain of sHsps because the quaternarystructure ... vivo studies of speciﬁc inter-actions between MTB HSP 16.3 and other latent stageproteins will lead to a b etter u nderstanding of the molecularchaperone activity of MTB HSP 16.3. Further ... The digestion pattern of MTBHSP 1 6.3 was similar in the absence and presence of ATP,where two major proteolytic fragments at Mr 8and 13 kDa were observed. The speciﬁcity of the effect of...

Tài liệu Báo cáo khoa học: Aldehydes release zinc from proteins. A pathway from oxidative stress⁄lipid peroxidation to cellular functions of zinc pptx

... zinc and MT protect the liver and the heartagainst the toxic effects of ethanol [4 5–4 7]. The aboveresults suggest that a critical aspect of the protectivefunction of MT is the scavenging of the ... demonstrates that elevated levels of aldehydesaffect zinc metabolism and that zinc release and ensu-ing binding of zinc to other proteins is one aspect of the molecular actions of aldehydes that are ... none of the aldehydes affects the absorbance of PAR. The data for the reaction of MT with formalde-hyde were corrected for the absorbance changes in the absence of MT.Thiol reactivities of MT and...

Tài liệu Báo cáo khoa học: Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane pdf

... addition of ISP and Qcr10p).On the other hand, in the transition from the 500 kDaband to the 670 kDa band, a structural rearrangement of the bc1complex may occur due to the binding of ISP and ... leu 2–3 ,112, can 1–1 1, qcr9D2::HIS3 [53]DISP MATa, ade 2–1 , his 3–1 1,15, trp 1–1 , leu 2–3 ,112, ura 3–1 , can 1–1 00, rip1D::LEU2 Present studyDBCS1 MATa, ade 2–1 , his 3–1 ,15, leu 2–3 ,112, trp 1–1 , ura 3–1 , ... speciﬁcallyrequired for the insertion of ISP into an immature bc1complex and that the association of the Qcr9p subunitwith the bc1complex precedes the binding of ISP. Infact, the direct absence of ISP (DISP),...

Tài liệu Báo cáo khoa học: Aggregative organization enhances the DNA end-joining process that is mediated by DNA-dependent protein kinase pdf

... nuclearfraction (NF) (lane 2), but not in DNA affinity- and Mono Q-fractions that EJ activity are enriched (lanes3 and 4). On the other hand, the ligase componentswere found in a Heparin column fraction, ... [23, and ref-erences therein].DNA aggregation activity is coupled withEJ activity To further confirm the relationship between protein–DNA aggregation and EJ activity, we fractionated the flow-through ... regard to the action mode, the role of the N-terminal portion of nucleolin is suggestive.This region includes the basic and repeated octapep-tides that are similar to histone H1 [28] and acidicstretches...

Báo cáo khoa học: Peptides from puriﬁed soybean b-conglycinin inhibit fatty acid synthase by interaction with the thioesterase catalytic domain pot

... further understand the structure–potency relation-ship of the FAS inhibitory peptides, we examined the relationships between the physicochemical and biochemical features of these peptides and their ... RKQEEDEDEEQQRE and C75 and their interaction energies with the TEdomain showed a strong correlation (r = 0.99).Close-up views of the binding modes of SBC-derived peptides and Orlistat with the TE active ... werecustom synthesized and their FAS inhibitory activitiestested. Figure 1 shows the FAS inhibitory activity of the three synthetic peptides and the C75 positive con-trol. The FAS inhibitory...

Báo cáo khoa học: A strategy for the generation of speciﬁc human antibodies by directed evolution and phage display An example of a single-chain antibody fragment that neutralizes a major component of scorpion venom docx

... of these changes occurred at frame-work 3 of the heavy chain (Asp74Asn) and 3 of the lightchain. Two of the changes (Thr152Ile and Ser197Gly)occurred at frameworks 1 and 3, respectively, and ... process beginswith the cloning and expression of cDNAs encoding the variable regions of the H and L chains of antibod-ies (VH and VL), allowing the in vitro generation of Keywordsaffinity ... biosensor of molecular interactions in realtime (BIACORE). Table 1 shows the values obtainedfor the binding kinetic constants. The affinity constants of both scFvs were similar, in the range of 10)7m.Affinity...

Báo cáo khoa học: "Medical treatment for the terminally ill: the ‘risk of unacceptable badness’"

... reasonable trial hasdemonstrated that its benefit has passed the point of diminishing returns. However, there is a continuing trend of surrogates demanding that moribund patients be kept on ... maintaining some of the vitalfunctions of the body, but the same technology does notnecessarily allow us to heal underlying disease processes [2].An unintended side effect of modern technological ... animation; they arenot alive in the sense the we enjoy life but neither are theyable to die as long as nutrition, hydration, ventilation, and perfusion are assured. In many cases reanimation of suchpatients...

Tài liệu Báo cáo khoa học: Dmrt1 genes at the crossroads: a widespread and central class of sexual development factors in ﬁsh pdf

... embryo-genesis, all the evidence suggests that sex determinationmight disobey the conventional rules of evolutionaryconservation. The common picture emerging here is that the genes at the top of the cascade ... result, the common emerg-ing picture is that the genes at the top of the cascade are not conserved,whereas the downstream genes have homologues in a much broader spec-trum of species. Among these ... birds, mammals and corals is the dmrt genefamily [5]. The prototype members of this group of factors are the Drosophila doublesex (dsx) and Caenor-habditis mab-3 genes. The Dmrt group of molecules...

Tài liệu Báo cáo khoa học: Nucleolin/C23 mediates the antiapoptotic effect of heat shock protein 70 during oxidative stress pptx

... the condensation and fragmentation of theirnuclei. The percentage of apoptotic cells was calculated as the number of apoptotic cells divided by the total number of cells counted. A minimum of ... lost.Taken together, our results demonstrate an impor-tant relationship among the regulation of nucleo-lin ⁄ C23, the activation of caspase-3 and the induction of apoptosis under the setting of oxidative ... events of caspase activation, the release of cathepsins, cytochrome c, apoptosis-inducing factor and even the death-associated lysosome permeabiliza-tion [1 7–2 1]. Particularly, Hsp70 inhibits the...

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