... 23–43 V for optimal fragmentation and, typically, 40–43 V was used for CID of [M-H]–, and 23–27 V for [M-H]2–.Ascanrateof1.5sperscanwasusedforbothES-MSandCIDMS/MSexperiments, and the acquired ... Leadeterminant.As the C3aion is at m/z 690, this excludes the possibility of the other Fuc being at the Gal next to the subterminalGlcNAc. The position of the second Fuc 3-linked to the internal GlcNAc forming ... Glc, the D-typecleavage occurs at the glycosidic bonds of both reducing and nonreducing sides of the residue, by combined C-type and Z-type cleavages (see Results for discussion and Figs 1–4 for illustration)....