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Báo cáo Y học: Purification, crystallization, NMR spectroscopy and biochemical analyses of a-phycoerythrocyanin peptides pptx

Báo cáo Y học: Purification, crystallization, NMR spectroscopy and biochemical analyses of a-phycoerythrocyanin peptides pptx

Báo cáo Y học: Purification, crystallization, NMR spectroscopy and biochemical analyses of a-phycoerythrocyanin peptides pptx

... alyase-isomeraseforthephotoactive31-Cys-a84-phycoviolobilinchromophore of phycoerythrocyanin. Biochemistry 40, 12444–12456.Ó FEBS 2002 Analyses of a-phycoerythrocyanin peptides (Eur. J. Biochem. 269) 5055 Purification, crystallization, ... 5055 Purification, crystallization, NMR spectroscopy and biochemical analyses of a-phycoerythrocyanin peptides Georg Wiegand1, Axel Parbel2*, Markus H. J. Seifert1, Tad A. Holak1 and Wolfgang Reuter11Max-Planck-Institut ... of a phycobiliprotein lyase: both the attachment of phy-cocyanobilin and the isomerization to phycoviolobilin are cata-lyzed by the proteins PecE and PecF encoded by thephycoerythrocyanin operon....
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Báo cáo khoa học: Purification, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris ppt

Báo cáo khoa học: Purification, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris ppt

... polyadenylation signal tobe obtained by PCR and sequencing. The deduced amino-acid sequence of P. hilaris cellulase showed high homologyto members of glycosyl hydrolase family 5 subfamily 2, ... a pen-type pH meter(model B-212; Horiba, Kyoto, Japan).Enzyme assayA carboxymethylcellulase (CMCase) assay was performedby measuring the amount of reducing sugars after incuba-tion of 100 ... detected three proteins bands by CBBstaining and one activity band by activity staining. Proteinsincluding cellulase activity were eluted and concentratedfrom the unstained gel, and the protein solution...
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Tài liệu Báo cáo Y học: Purification, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase pot

Tài liệu Báo cáo Y học: Purification, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase pot

... 7.4,5mMazide inhibited ADP hydrolysis by  70% whereasATP hydrolysis was inhibited by only 10%. Inhibition of ADP and ATP hydrolysis by azide was greater when the pH of assay solutions was 6.4. Azide inhibition ... 0.2 and 0.64 depending on the pH and the presence of NP-40; (d)Fig. 3. Inhibition of ATP and ADP hydrolysis activities of purifiedchicken liver ecto-ATPDase by azide. ATP and ADP hydrolysis ... inhibition by these compounds. Pyro-phosphate was previously shown to be a competitiveinhibitor of the oviduct ADPase activity [32], whereasFig. 2. Effect of pH on ATP and ADP hydrolysis by the purified...
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Tài liệu Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt

Tài liệu Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt

... residues of the C. sepium b-amylase (Cys83,Cys96, Cys209 and Cys344) are homologous to those foundin soybean b-amylase (Cys82, Cys97, Cys208 and Cys343).On the analogy of the soybean b-amylase, ... method.Inhibition of the enzyme activity by glucose, maltose and cyclohexaamyloseFor the study of the enzyme inhibition by glucose, maltose and cyclohexaamylose b-amylase activity was measuredusing ... France;3Institute of PlantBiochemistry, Halle, Germany;4Laboratory of Developmental Physiology and Molecular Biology, Katholieke Universiteit Leuven, Leuven,BelgiumAn abundant catalytically active b-amylase...
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Báo cáo Y học: Purification and biochemical characterization of some of the properties of recombinant human kynureninase pptx

Báo cáo Y học: Purification and biochemical characterization of some of the properties of recombinant human kynureninase pptx

... Purification and biochemical characterization of some of the properties of recombinant human kynureninaseHarold A. Walsh and Nigel P. BottingSchool of Chemistry, University of St Andrews, St Andrews, ... regulation of enzyme activity in vivo and consequent channeling of substrate 3-hydroxykynurenine down the tryptophan–kynurenine metabolic pathway.The purification of recombinant human kynureninaseto ... pathophysiology of human diseaseshas always been hampered by the availability of humantissue, aesthetics, and ethical considerations. The principleaim of this study was to express a clone of humankynureninase...
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Báo cáo Y học: Purification and characterization of novel kininogens from spotted wolffish and Atlantic cod pdf

Báo cáo Y học: Purification and characterization of novel kininogens from spotted wolffish and Atlantic cod pdf

... Kalkkinen11Institute of Biotechnology, Protein Chemistry Laboratory, University of Helsinki, Finland,2Norwegian College of Fishery Science,University of Tromsø, Norway,3Institute of Medical Biology, University ... R3 (PerSeptive Biosystems,Framingham MA, USA) tip, and a sample of the flow-through carrying the glycans was drop-dialysed and analysed by MALDI-TOF MS.RESULTSIsolation of cysteine proteinase ... biantennary and triantennary N-glycans that are terminated by a2,3-linkedsialic acids, a high number of which are O-acetylated. About1/3 of the glycans carry sulfate at N-acetylglucosamine unitsof...
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Báo cáo Y học: Purification and characterization of three galactose specific lectins from Mulberry seeds (Morus sp.) ppt

Báo cáo Y học: Purification and characterization of three galactose specific lectins from Mulberry seeds (Morus sp.) ppt

... inhemagglutination, lymphocyte transformation, inactivation of certain types of tumor cells and precipitation of certainpolysaccharides and glycoproteins [1,2]. Plant lectinsisolated from a wide variety of plants ... molecular mass of 70 000 Da, while theMSL-2 and MSL-3 gave four bands with molecular masses of 42 000, 35 000, 25 000 and 19 000, and 35 000, 22 500,17 000 and 14 950 Da, respectively.Affinity for Sepharose ... nonionic hydrophobicinteractions.The lectins agglutinated rat red blood cells and thisagglutination was inhibited specifically by galactose,methyl-a-D-galactopyranoside, methyl-b-D-galactopyrano-side,...
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Báo cáo Y học: Purification and characterization of VanXYC, a D,D-dipeptidase/D,D-carboxypeptidase in vancomycin-resistant Enterococcus gallinarum BM4174 docx

Báo cáo Y học: Purification and characterization of VanXYC, a D,D-dipeptidase/D,D-carboxypeptidase in vancomycin-resistant Enterococcus gallinarum BM4174 docx

... mutagenesis [20], and later confirmed byanalysis of the crystal structure [19]. Comparison of theactive site of VanXYCwith VanX- and VanY-type enzymesindicated that all of these enzymes contained ... parameters of D59A for hydrolysis of UDP-MurNAc-pentapeptide[Ala]. However, its activitywas comparable with that of D59S and His6–VanXYC.Therates of hydrolysis of D-Ala-D-Ala and UDP-MurNAc-pentapeptide[Ala] ... specific-ally hydrolyseD-Ala-D-Ala with minimal activity againstD-Ala-D-Ser, a very different type of specificity.VanXYC, VanX-type and VanY-type enzymes containmost of the active site...
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Báo cáo Y học: Purification and properties of the extracellular lipase, LipA, of Acinetobacter sp. RAG-1 docx

Báo cáo Y học: Purification and properties of the extracellular lipase, LipA, of Acinetobacter sp. RAG-1 docx

... [6].LipA demonstrates hydrolytic activity toward emulsions of both medium and long chain triacylglycerols (Fig. 3).Areas of olive oil and tricaprylin hydrolysis are clearly seen and correspond with ... preferentiallyattacks medium length acyl chains (C6,C8). The enzymedemonstrates hydrolytic activity in emulsions of bothmedium and long chain triglycerides, as demonstrated byzymogram analysis. ... ester hydrolases (EC 3.1.1.3) thatcatalyze the hydrolysis of triacylglycerols to free fatty acids and glycerol. They resemble esterases in catalytic activity,but differ in that their substrates...
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Báo cáo Y học: Purification and catalytic properties of a CO-oxidizing:H2-evolving enzyme complex from Carboxydothermus hydrogenoformans doc

Báo cáo Y học: Purification and catalytic properties of a CO-oxidizing:H2-evolving enzyme complex from Carboxydothermus hydrogenoformans doc

... purification and catalytic proper-ties of a membrane-bound enzyme complex from C. hydro-genoformans composed of a hydrogenase and a COdehydrogenases. The complex catalyzes the conversion of CO to ... polypeptides arehydrophobic as shown by the chloroform/methanol extr-action experiment. These polypeptides were in-gel trypsin orCNBr digested and the peptides obtained were analysed byMALDI-TOF ... dehydrogenase,hydrogenase or both enzymes the influence of Ti(III)citrateon CO dehydrogenase- and hydrogenase activity was tested.In these assays methylene blue was used instead of methylviologen...
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