... includedSM22a, a known actin cross-linking protein, and BSA,which does not interact with or cross-link actin. In the absence of hhLIM, the majority of actin remained in the supernatant (S) and only a small ... dis-ruption of the second LIM domain of hhLIM abol-ished F-actin-binding activity, indicating the importantrole that LIM domain 2 plays in the control of assem-bly and organization of the actin cytoskeleton. In ... transport and cell growth, signaling, anddivision. Many of the actin-binding proteins affect the cytoskeletal structure and architecture by mediating the association of actin filaments into cables and...