Báo cáo khoa học: Endogenous mono-ADP-ribosylation mediates smooth muscle cell proliferation and migration via protein kinase N-dependent induction of c-fos expression potx

... blocked thestimulation of DNA and RNA synthesis, prevented smooth muscle cell migration and suppressed the induction of c-fos and c-myc gene expression. An examination of relevantsignal transduction ... MAP kinase, Rho mediates growth factor-dependent activation of c-fos via the serum response factor (SRF) [30]. Protein kinase C-related kinase 1 (PRK1), also termed protein kinase N (PKN), and ... inhibition and rapid depolymerization. Biochemistry 31, 310–316.110 L. Yau et al. (Eur. J. Biochem. 270) Ó FEBS 2003 Endogenous mono-ADP-ribosylation mediates smooth muscle cell proliferation and migration...

Báo cáo khoa học: Aromatic amino-acid residues at the active and peripheral anionic sites control the binding of E2020 (AriceptÒ) to cholinesterases doc

... mutants of the implicatedresidue in two template enzymes. Differences in thereactivity of E2020 toward AChE and BChE and acomparison of KIvalues of E2020 for mouse (Mo) AChEmutants of Trp86(84), ... ammoniumgroup of E2020 and the c-oxygen of Asp74 which areseparated by a distance of 5.4 A˚;(c)ap–p interactionbetween the indanone ring of E2020 and Trp286 in theperipheral anionic site of AChE; ... p–p interaction of the phenyl ring of E2020 with W82 of BChE; (b) a strong electrostatic interaction between the charged ammonium nitrogen of E2020 and the c-oxygen of D70(72)ofBChE.Thesetwointeractions...

Tài liệu Báo cáo khoa học: Nucleolin/C23 mediates the antiapoptotic effect of heat shock protein 70 during oxidative stress pptx

... Apoptotic cells wereidentiﬁed by the condensation and fragmentation of theirnuclei. The percentage of apoptotic cells was calculated asthe number of apoptotic cells divided by the total number of cells ... protein expression and mRNA stability in the presence or absence of H2O2. (A) Westernblot analyses showing the effect of nucleolin ⁄ C23 on Bcl-2 protein expression. Total cellular proteins ... phos-phoprotein. It is abundantly expressed in both normal and cancerous cells [9], and ubiquitously distributed inthe nucleolus, nucleus and cytoplasm of the cell, aswell as at the cell surface...

Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx

... members of the Ras family of small G proteins.The activity of Ras proteins is under tight control of several classes of guanine nucleotide exchange factors(GEFs) and GTPase-activating proteins. ... 5-HT7receptors induced protein kinase A-dependent phosphorylation of the endogenous human Ras-GRF1on Ser927 and of transfected mouse Ras-GRF1 on Ser916. Ras-GRF1overexpression increased basal and serotonin-stimulated ... mitogen-activated protein ⁄ extracellular signal-regulated kinase kinase; PKA, protein kinase A; Sos1,son of sevenless 1.2304 FEBS Journal 272 (2005) 2304–2316 ª 2005 FEBSexpressed in mammalian cells...

Báo cáo khoa học: Endogenous tetrahydroisoquinolines associated with Parkinson’s disease mimic the feedback inhibition of tyrosine hydroxylase by catecholamines doc

... atomtypes and Gasteiger atomic charges were assigned to theligand atoms [59]. We limited the output to a maximum of 200 placements per ligand; the (R) and (S) enantiomers of NMNorsal and NMSal ... °C. Phosphorylation by protein kinase A facilitates the release of bound catecholamines and is the mostprominent mechanism of tyrosine hydroxylase reactivation. Protein kinase A also fully restored ... charged nitrogen atoms of NMTIQ and MPTP and the backbone oxygen of Ser324. The dis-tances between the nitrogen atoms and the oxygen of Ser324 were 2.01 A˚for NMTIQ and 2.26 A˚forMPTP,...

Báo cáo khoa học: MicroRNA-23b mediates urokinase and c-met downmodulation and a decreased migration of human hepatocellular carcinoma cells doc

... the 3¢-UTR of uPA and of c-met mRNAs and translationally repress the expression of uPA and c-met in HCC cells.The evidence obtained shows that overexpression of miR-23b leads to uPA and c-met ... 3 and 6, and lanes 2 and 5,Fig. 3. miR-23b inhibits uPA and c-met protein expression in SKHep1C3 cells. (A) Western blot analysis of uPA in the conditioned media of control cells (lanes 1 and ... Petro1 and Sergio Barlati11 Division of Biology and Genetics, Department of Biomedical Sciences and Biotechnology, IDET Centre of Excellence, University of Brescia,Italy2 Department of Biology and...

Tài liệu Báo cáo khoa học: Dmrt1 genes at the crossroads: a widespread and central class of sexual development factors in ﬁsh pdf

... highincidence of teratomas and resulted in a failure of germ cells to arrest mitosis [56]. Thus, Dmrt1 in mice and similarly Dmrt1bY in medaka appear to be regu-lators of germ cell proliferation. ConclusionTo ... germline expression is dispensable, condi-tional dmrt1 inactivation in either the Sertoli cells orthe germ cells indicated that mouse Dmrt1 is indeedrequired for radial migration of germ cells and ... and expression in gonads of zebraﬁsh Dmrt1. BiochemBiophys Res Commun 330, 950–957.25 Johnsen H, Seppola M, Torgersen JS, Delghandi M &Andersen O (2010) Sexually dimorphic expression of A....

Tài liệu Báo cáo khoa học: Hepatic stimulator substance mitigates hepatic cell injury through suppression of the mitochondrial permeability transition pdf

... (pcDNA3.0 alone) or wild-typecells (Fig. 1A). Protein expression of HSS in the three cell lines (untransfected cells, vector-transfected cells, and HSS-expressing cells) was detected using westernblot. ... incirrhotic human livers, and the mRNA level of HSSwas elevated in tissue samples of hepatocellular carci-noma and cholangiocellular carcinoma [5]. Thisincreased expression of HSS in liver tumors ... CsA, which is apotent inhibitor of MPT. Both CsA treatment and the expression of HSS decreased the number of apoptoticcells, and CsA alone was able to greatly alleviateCCCP-induced apoptosis.Apoptosis...

Tài liệu Báo cáo khoa học: Loose interaction between glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase revealed by ﬂuorescence resonance energy transfer–ﬂuorescence lifetime imaging microscopy in living cells doc

... case of cell samples without coexpression of GAPDH) or from the cytoplasmic area (in the case of cell samples with coexpression of GAPDH) of cells, and are expressed as mean ± standard deviation. ... 1. Subcellular localization and enzymatic activities of GAPDH–citrine and PGK–cerulean. (A) Expression of GAPDH–citrine and PGK–cerulean. Localization of endogenous GAPDH and PGK inHeLa cells ... (D) Expression of PGK and GAPDH chimeric proteins. Expression plasmids were transfectedinto CHO-K1 cells, and proteins weresubjected to SDS ⁄ PAGE, transferred tonitrocellulose membranes, and...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia: roles in human malignancies and potential therapy pdf

... transcription via activation of P-TEFb kinase. In line with this, after 5 days of induction, ectopic expression of AF4–MLL resulted inthe transcriptional deregulation of 660 genes, of which580 ... several other proteins. Thus, the pattern of proteins bound to both reciprocal MLL proteins is quitecomplex and will inﬂuence the biological properties of agiven MLL fusion protein. Known protein ... leukemia cells to survive.GSK3 is implicated in different signaling pathways,for example, protein kinase C, protein kinase A,RAS ⁄ RAF, WNT-, phosphatidylinositol 3 -kinase and Hedgehog, and thus...

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