Báo cáo khoa học: Binding of non-natural 3¢-nucleotides to ribonuclease A ppt

Báo cáo khoa học: Binding of activated Factor XII to endothelial cells affects its inactivation by the C1-esterase inhibitor pptx

... Initiation of activation of the system has been found to be a result of a slowautodigestion and autoactivation of surface bound FXII byFXIIa [2]. Binding of FXII to a negatively charged surfaceinduces ... doi:10.1046/j.1432-1033.2003.03367.xaccompany inﬂammation and tissue damage which isthought to play a major role in the symptomatology of acute attacks in patients with angiodema [12,28]. Binding of FXIIa to different negatively ... University of Copenhagen, DenmarkIt is well known that activated Factor XII (FXIIa) andkallikrein are rapidly inactivated in plasma as a result of reaction with endogenous inhibitors. The purpose of...

Báo cáo khoa học: "Applications of Automatic Evaluation Methods to Measuring a Capability of Speech Translation System" pot

... Applications of Automatic Evaluation Methods to Measuring a Capability of Speech Translation SystemKeiji YasudaATR Spoken Language Translation Research Laboratories2-2-2, Hikaridai,"Keihanna ... City",Kyoto, 619-0288, JapanSeiichi YamamotoMasuzo YanagidaATR Spoken LanguageDoshisha UniversityTranslation Research Laboratories1-3, Tatara-miyakodani, Kyotanabe,2-2-2, Hikaridai, ... Laboratorie2-1-15, Ohara, Kamifukuoka-city,Saitama, 356-8502, Japanfsugaya@kddilabs.jpToshiyuki TakezawaATR Spoken LanguageTranslation Research Laboratories2-2-2, Hikaridai, "Keihanna Science...

Báo cáo khoa học: Binding of non-natural 3¢-nucleotides to ribonuclease A ppt

... inter-action of RNase A and 3¢-nucleotides, and indicate that non-natural fura-nose rings can serve as the basis for more potent inhibitors of catalysis byRNase A. AbbreviationsaraUMP, arabinouridine ... was added,and subsequent additions again doubled in volume until a 32-lL aliquot had been added, for a total of nine additions(75.5 lL) altogether. In each assay, 15% of the substratewas cleaved. ... to RNase A FEBS Journal 272 (2005) 744–755 ª 2005 FEBS 755 Binding of non-natural 3¢-nucleotides to ribonuclease A Cara L. Jenkins1, Nethaji Thiyagarajan2, Rozamond Y. Sweeney3, Michael...

Báo cáo khoa học: Interaction of caspase-3 with the cyclic GMP binding cyclic GMP speciﬁc phosphodiesterase (PDE5a1) potx

... SDS/PAGE.Caspase-3 activity assaysCaspase-3 activity in cell lysates was measured using[35S]methionine-labeled PARP as a substrate. In each assay,equal amounts of cell lysate protein ( 5 lg/assay)were ... cleavage of intermediate fragments, as a result of particularly good overexpression of the enzyme inCos-7 cells. Cotransfection of PDE 5A1 did not affect theauto-activation of caspase-3. Caspase-3 ... has thesequence GAC CAA GGA GAT TGA GAG AGG AAAGAA CTC, while the reverse primer was GAG TTC TTTCCT CTC TCA ATC TCC TTG GTC. Twelve cycles of Fig. 1. Schematic showing the positions of caspase...

Tài liệu Báo cáo khoa học: Binding of N- and C-terminal anti-prion protein antibodies generates distinct phenotypes of cellular prion proteins (PrPC) obtained from human, sheep, cattle and mouse doc

... densitometry. The percentages of each band regarding to the total signal of PrPCwere calculated asarithmetic means of separate gel runs. The number of gel runs forthe analyses are given for each ... Gomez Parada M& Groschup MH (2000) Molecular analysis of Irishsheep scrapie cases. J General Virol 81, 1621–1627.36 Hayashi HK, Yokoyama T, Takata M, Iwamaru Y,Imamura M, Ushiki YK & ... of the di- (d), mono- (j)and nonglycosylated isoform (m) of PrPCwere quantiﬁed and calcu-lated as percentages of the total signal. The glycoforms of the pro-tein bands were analyzed as arithmetic...

Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx

... Aiba Y, Nakamura K, Namba T, HirataM, Mazda O, Katsura Y & Narumiya S (1993)Thromboxane A2 receptor is highly expressed in mouseimmature thymocytes and mediates DNA fragmentationand apoptosis. ... pGL3e:Prm3aab. (PrimerKin160; 5¢-dGAGAGGTACCGCAAATCTTCTCTCGCCTCC-3¢, corresponding to NTs )106 to )86).(8) Prm3aaa; pGL3b:Prm3aaa & pGL3e:Prm3aaa. (PrimerKin161, 5¢-dGAGAGGTACCGCAGCATCGGCCTGATGGG-3¢, ... 5¢-dGAGAGGTACCTAGGAGTTCACCAGAGC-3¢, corresponding to NTs )154 to )137).(5) Prm3ax; pGL3b:Prm3ax & pGL3e:Prm3ax. (PrimerKin177; 5¢-dGAGAGGTACCAGCTACTTACACTGAAATGCAG-3¢, corresponding to...

Tài liệu Báo cáo khoa học: Binding of ligands originates small perturbations on the microscopic thermodynamic properties of a multicentre redox protein pptx

... anthraquinone-2-sulfonate, safranine O, diquat, neutral red, phenosafranine,and methylviologen, all at a ratio of 100 : 8 of cytochromevs. mediator to avoid interference caused by speciﬁc binding of mediators to ... Delgado R, Frausto da Silva JJR, Amorim MTS,Cabral MF, Chaves S & Costa J (1991) Dissociationconstants of Bronsted acids in D2O and H2O: studieson polyaza and polyoxa–polyaza macrocycles ... effect of complex forma-tion. Because the thermodynamic parameters for thehaems calculated from the NMR data are relative, itcould be argued that all haem potentials had beenmodiﬁed to a similar...

Tài liệu Báo cáo khoa học: Inhibition of glyceraldehyde-3-phosphate dehydrogenase by peptide and protein peroxides generated by singlet oxygen attack docx

... can also arise via radicalTable 2. Percentage of enzyme activity retained after incubation of GAPDH, GR and LDH with N-Ac-Trp-OMe peroxides or H2O2at 37 °Cintheabsence, or presence, of a ... (¤)Catalase added; (h) no catalase added. Initial peroxide concentrationswere in the range of 420–520 lMfor RNase A, 540–660 lMforN-Ac-Trp-OMe, and 130–180 lMfor lysozyme. Data are means± ... in a very rapid initial decrease in peroxideconcentration, and a subsequent slow decay (Fig. 1). Theabsolute levels of peroxide lost in the rapid phase afteraddition of catalase, was substrate...

Báo cáo khoa học: Binding of ATP at the active site of human pancreatic glucokinase – nucleotide-induced conformational changes with possible implications for its kinetic cooperativity doc

... berelated to partial catalytic activation following(Mg)ATP binding at physiological concentrations of Glc. Similar or possibly larger effects of ATP in promot-ing a catalytically competent state ... glucokinase cause hypoglyca-emia- and hyperglycaemia syndromes and their analysisilluminates fundamental quantitative concepts of glu-cose homeostasis. Diabetologia 42, 1175–1186.21 Mahalingam B, ... b-cells, and catalytic activation by a- D-glucose binding has a key regulatory function. Whereas the mechanism of this activation is wellunderstood, on the basis of crystal structures of human GK,...

Báo cáo khoa học: Binding of cGMP to the transducin-activated cGMP phosphodiesterase, PDE6, initiates a large conformational change involved in its deactivation ppt

... S,Komori N, Matsumoto H, Matsuura I, Hayashi F,Yamazaki RK & Usukura J (2010) Mechanism for theregulation of mammalian cGMP phosphodiesterase 6.2: isolation and characterization of the transducin-activated ... cGMP to Pabc is not involved PDEactivation Binding of [3H]cGMP to Pabc implies that cGMP binding may be involved in the activation of P abcc to Pabc and ⁄ or in the deactivation of Pabc to Pabcc.Here, ... or absent, and that the releasedPc accelerates deactivation of GTP–Ta-activated PDE.These implications are also problematical. First, Pc isreleased from Pabcc to accelerate PDE deactivation.This...

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