Báo cáo khoa học: Identiﬁcation and expression analysis of an IL-18 homologue and its alternatively spliced form in rainbow trout (Oncorhynchus mykiss) doc

... Biochem. 271) 1923 Identiﬁcation and expression analysis of an IL-18 homologue and its alternatively spliced form in rainbow trout (Oncorhynchus mykiss)Jun Zou1, Steve Bird1, Jonathan Truckle1, ... alternativesplicing may play an important role in regulating IL-18 activities in rainbow trout. Keywords: interleukin 18; alternative splicing; expression; rainbow trout. In the last few years, major advances ... Genomic DNA and the deduced proteinsequence of rainbow trout IL-18. Exons and introns are indicated in uppercase and lower-case, respectively. Intron splicing signal motifsare boxed and the polyadenylation...

Tài liệu Báo cáo khoa học: Identiﬁcation of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx

... C30 terpe-nes and include a number of active constituents of medicinal plants, as exempliﬁed by glycyrrhizin in Glycyrrhiza glabra, ginsenosides in Panax ginseng, sai-kosaponins in Bupleurum ... [50], and oleanolic acid and ursolic acid show anti -in am-matory and antitumor-promoting activities [51], etc.As the supply of triterpenes including oxygenatedderivatives through organic synthesis ... acetylated, and analyzed using GC-MS fol-lowing the same procedure as described above.Production of olean-12-ene-3b,24-diol bycoexpression of CYP93E and b-amyrin synthase in lanosterol synthase-deﬁcient...

Báo cáo khoa học: Identiﬁcation and functional expression of a second human b-galactoside a2,6-sialyltransferase, ST6Gal II docx

... SP1 and TBPbinding sites found upstream exon B. This ﬁnding wouldargue for a ubiquitous and low-level expression of exon Bcontaining transcripts. On the other hand, the presence of CREB and ... (1988) Asparagine-linked oligo-saccharides on lutropin, follitropin, and thyrotropin. I. Structuralelucidation of the sulfated and sialylated oligosaccharides onbovine, ovine, and human pituitary ... sequence linked to a protein. The a2,6-linkagewas conﬁrmed by the increase of Sambucus nigra agglutinin-lectin binding to the cell surface of CHO transfected withthe cDNA encoding ST6Gal II and...

Báo cáo khoa học: Identiﬁcation and expression of the ﬁrst nonmammalian amyloid-b precursor-like protein APLP2 in the amphibian Xenopus laevis ppt

... luminal portions of all superfamilymembers contain conserved zinc-binding [13,14], heparin-binding [14] and collagen-binding domains [15]. Further-more, a GTP-binding protein (G0) binding ... in only human and rodents.APP and the APLPs are type-I transmembrane proteinscontaining a signal peptide, a large amino-terminal luminal/extracellular part, a transmembrane domain and a shortcytoplasmic ... secondheparin-binding domain [3,4,13,26].Comparative analysis of theXenopus and mammalianAPLP2 proteinsComparing the amino acid sequences of the twoX-APLP2 proteins with the human, mouse and ratAPLP2...

Báo cáo khoa học: Identiﬁcation of a novel thyroid hormone-sulfating cytosolic sulfotransferase, SULT1 ST5, from zebraﬁsh Molecular cloning, expression, characterization and ontogenic study ppt

... being a neutral compound and l-T3, with its amino and carboxyl groups, being a charged molecule)or, in fact, reflect distinct catalytic mechanisms remainsto be clarified. Several endogenous and ... thyroidhormones and their metabolites. It will be interestingto investigate whether SULT1 ST5 plays a unique and important role in the metabolism and homeostasis of thyroid hormones in vivo.To investigate ... 50%identical to human and dog SULT1B1 (thyroid hormone SULT). Arecombinant form of zebrafish SULT1 ST5 was expressed using thepGEX-2TK bacterial expression system and purified from transformedBL21...

Báo cáo khoa học: Identiﬁcation, structure and differential expression of novel pleurocidins clustered on the genome of the winter ﬂounder, Pseudopleuronectes americanus (Walbaum) ppt

... of antimicrobial peptide candidates for bothhuman and nonhuman therapeutants from genomicsequences and will aid in understanding the evolution and transcriptional regulation of expression of ... cloned and sequenced from intestine [5] and immuno-gold electron microscopy revealed WF2-like pleurocidintranscripts in mucus-producing cells of skin and goblet cells of small intestine in winter ... thedifferent pleurocidin genes; expression of the multiple genesencoding pleurocidins or, in fact any antimicrobial peptide in any organism, during different stages of development and in different tissues...

Tài liệu Báo cáo khoa học: Identiﬁcation of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt

... with EWS (lanes 2, 4 and 6) and 32P-labeled Htelo (lanes 3 and 4), dsHtelo (lanes 5 and 6) or ssDNA S (lanes 1 and 2). Thestructures of DNAs used as probes are indicated above each lane.The ... Protein–nucleic acid complexes. (A) EMSA was performedwith RGG3 (lanes 2 and 4) and 32P-labeled Htelo (lanes 1 and 2) orrHtelo (lanes 3 and 4). (B) EMSA was performed with RGG3 (lanes2 and ... isinvolved in transcription, rRNA processing and ribo-some assembly, can bind to G-quadruplex DNAformed from the external transcribed spacer region of human rDNA, ETS-1. We performed an EMSA of EWS and...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebraﬁsh, Danio rerio docx

... Horne M & SecombesC (2004) Identiﬁcation and expression analysis of an IL-18 homologue and its alternatively spliced form in rainbow trout (Oncorhynchus mykiss). Eur J Biochem271, 1913–1923.33 ... expression of T-bet has beenseen within a wide variety of tissues and cells in theGinbuna crucian carp [27] and, in mammals, in thelung tissue of mouse and spleen and thymus of human and mouse ... seen in the putative T-boxDNA-binding domain of t-bet, the STAT protein inter-action domain, STAT protein all-alpha domain, STATprotein DNA-binding domain and SH2 domain of stat6, and the zinc-ﬁnger...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf

... gray and dark gray, respectively, and the regions representing the 5¢- and 3¢-UTRs areshown in white in the genomic gene and the cDNA. Intron size in bp, domain size in bp and domain size in amino ... protein (BMP), and acti-vin [8–11]. The TGFb signaling cascade is stimulatedthrough the binding of ligand to a type II receptor, atransmembranous serine ⁄ threonine receptor kinase.The ligand–type ... properties of SmSmad1 and SmSmad1B asdescribed above. However, in the presence of SmSmad4 and the active mutant form of TbRI, both SmSmad2 and SmSmad4 interacted with GCN5, indicating theformation of...

Tài liệu Báo cáo khoa học: Identiﬁcation, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx

... metazoan CA protein sequences. Iden-tical and similar amino acids shared by at least 50% of the isoforms are shown in black and grey, respectively. Histidine residues involved in zinc binding in ... processes in which they participate, CA can play a signiﬁcantrole in autotrophic organisms, serving as an inorganiccarbon-concentrating component [12]. In symbiosisinvolving metazoa and autotrophic ... able to transfer protons [31].However, without an overexpression approach of RpCAbr and RpCAtr, we cannot know the functionaleffect of changes of some key amino-acids.Origin and number of nonvertebrate...

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