Báo cáo khoa học: Complexes of Thermoactinomyces vulgaris R-47 a-amylase 1 and pullulan model oligossacharides provide new insight into the mechanism for recognizing substrates with a-(1,6) glycosidic linkages docx

... Complexes of Thermoactinomyces vulgaris R-47 a-amylase 1 and pullulan model oligossacharides provide new insight into the mechanism for recognizing substrates with a- (1, 6) glycosidic linkages Akemi ... accepted 11 October 2005)doi :10 .11 11/ j .17 42-4658.2005.05 013 .x Thermoactinomyces vulgaris R-47 a-amylase 1 (TVAI) has unique hydrolyz-ing activities for pullulan with sequence repeats of a- (1, 4), ... hydrolysea- (1, 6) glycosidic linkages: the ratio of activity of hydrolysis for a- (1, 4) and a- (1, 6) glycosidic linkages is27 : 1 [3]. We reported the crystal structures of TVAI[4], a complex of TVAI with...

Tài liệu Báo cáo khoa học: Synchronization of Ca2+ oscillations: a capacitative (AC) electrical coupling model in neuroepithelium pptx

... wiring of the nervous system. Proc NatlAcad Sci USA 10 1, 5 311 –5 312 . 21 Fujita S (2003) The discovery of the matrix cell, the identiﬁcation of the multipotent neural stem cell and the development of ... (Fig. 1D). The electrical circuits of the current loop are presented in Doc. S2 of [5]. The ﬂuctuation in the membrane potential of the Ca2+store may be due to the movement of Ca2+ions and the ... between the outer nuclearmembrane (ONM) and inner nuclear membrane(INM) [1 3]. Intracellular Ca2+stores are formedwithin the ER lumen and the space between the ONM and the INM [1, 2]. In cells with...

Tài liệu Báo cáo khoa học: Roles of heat shock factors in gametogenesis and development pptx

... Steril 90, 11 09 11 18.Role of the HSF family in development R. Abane and V. Mezger 416 6 FEBS Journal 277 (2 010 ) 415 0– 417 2 ª 2 010 The Authors Journal compilation ª 2 010 FEBS 10 Lachance C, Fortier ... reported in Hsf1tm1Anak ⁄ tm1Anak[86],Hsf1tm1Miv ⁄ tm1Miv[72] and Hsf1tm1Ijb ⁄ tm1Ijb[92] mice(Table 1) . However, double-knockout Hsf1tm1Miv ⁄tm1Miv⁄ Hsf2tm1Miv ⁄ tm1Mivleads to male ... unravel the speciﬁc targets of HSF1 inspermatogenesis and to estimate the proportion of com-R. Abane and V. Mezger Role of the HSF family in developmentFEBS Journal 277 (2 010 ) 415 0– 417 2 ª 2 010 The...

Tài liệu Báo cáo khoa học: Effect of siRNA terminal mismatches on TRBP and Dicer binding and silencing efﬁcacy pdf

... speciﬁc silencing of the intended target, loading of the appropriate guidestrand into the RISC is critical. Improved understand-ing of the interactions of siRNAs with TRBP and Dicerwill enable ... binds to the more sta-ble end of the siRNA duplex and directs binding of Dicer-2 to the other, less stable, end, and hence the guide strand is selected through interaction of its 5¢ end with Dicer-2 ... Acad Sci USA 10 2, 12 135 12 140.34 Pellino JL, Jaskiewicz L, Filipowicz W & SontheimerEJ (2005) ATP modulates siRNA interactions with anendogenous human Dicer complex. RNA 11 , 17 19 17 24.35...

Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

... successfully in former studies, leading to the identification of the two first C. gigas members of the GH18 family, Cg-Clp1 (AJ9 712 41) [5] and the chi-tinase Cg-Chit (AJ9 712 38) [15 ]. The complete 16 97 bpcDNA ... characterizedby the degeneration of larval tissues, such as the velum and the foot, and the remodelling of larval tissues toproduce adult tissues (i.e. the development of the gills and the production of ... Hanks -19 9medium modified by the addition of 250 mm NaCl, 10 mmKCl, 25 mm MgSO4, 2.5 m m CaCl2, and 10 mm Hepes; the final pH was 7.4, and the osmolarity was 11 00 mOsmolÆ L )1 .Cells were then...

Tài liệu Báo cáo khoa học: Role of transcription factor activator protein 1 (AP1) in epidermal growth factor-mediated protection against apoptosis induced by a DNA-damaging agent doc

... Osaka573- 010 1, JapanFax: + 81 72 866 311 7Tel. + 81 72 866 311 8E-mail: takeuchi@pharm.setsunan.ac.jp(Received 31 March 2006, revised 3 June2006, accepted 14 June 2006)doi :10 .11 11/ j .17 42-4658.2006.05377.xWe ... Japan).Isolation of the cytosolic fractionCells were pretreated or not with 10 0 ngÆmL )1 of EGF for 48 h and thereafter with 20 lm ADR for deﬁned times. Theywere then washed twice with NaCl ⁄ Pi, and ... 10 3 11 1. 17 Meisenhelder J, Suh PG, Rhee SG & Hunter T (19 89)Phospholipase C-cis a substrate for the PDGF and EGF receptor protein-tyrosine kinases in vivo and in vitro. Cell 57, 11 09 11 22. 18 ...

Tài liệu Báo cáo khoa học: Association of mammalian sterile twenty kinases, Mst1 and Mst2, with hSalvador via C-terminal coiled-coil domains, leads to its stabilization and phosphorylation doc

... including the novel Ras effector 1, Nore1 [15 ,16 ], the putative tumour suppressor (Ras suppres-sor factor 1; Rassf1) [15 ,16 ,25], and most recently,Raf1 (with Mst2) [26]. The association of Mst with Nore1 ... Mst1 and Mst2. The mutation of K59R in the ATP-binding region of the Mst1 kinasedomain renders the kinase inactive [7 ,11 ,12 ,16 ], and byhomology with Mst1 the analogous mutation of K56Rin Mst2 ... Nore1 and Rassf1maintain Mst1 activity at low or basal levels it hasbeen shown that Mst1 in complex with either Nore1 or with Rassf1 bound to the scaffold protein, connectorenhancer of KSR1,...

Tài liệu Báo cáo khoa học: Modulation of oat arginine decarboxylase gene expression and genome organization in transgenic Trypanosoma cruzi epimastigotes docx

... digestion of the DNA samples.On the other hand, the absence of a second hybridiza-tion band in lane 1 might be the result of insufﬁcientsensitivity of our assay. Rehybridization of the mem-brane ... 9, 11 and 13 show the PCR products obtained using the primer pair T7 and RIB. Lanes 2, 4, 6, 8, 10 , 12 and 14 show the PCR products obtained using the primer pair T7 and ADC2. Lane 15 , 1 kb DNA ... 2006 FEBSactive bands (Fig. 4B), providing further support for the integration of entire molecules of the transformingplasmid, pADC-8.To investigate further the fate of the transformingplasmid...

Tài liệu Báo cáo khoa học: "TREATMENT OF LONG DISTANCE DEPENDENCIES IN LFG AND TAG: FUNCTIONAL UNCERTAINTY IN LFG IS A COROLLARY IN TAG" ppt

... adjoining the statements t, and troot hold for the node corresponding to the root of the auxiliary tree. Similarly, the statements b, and b/oo~ hold for the node corresponding to the foot of the ... that troo~,broot and tloot= bLoo~ are the t and b statements of the root and foot nodes of the auxiliary tree used for adjoining at the node 7. Based on what t and b stand for, it is obvious ... ently, the adjoining sequence at the root is inde- pendent of other nodes in the tree in spite of the coindexing. This is due to the fact that as the fea- ture structure of the foot of/ ~1 gets...

Báo cáo khoa học: Effect of ﬂanking bases on quadruplex stability and Watson–Crick duplex competition pptx

... in the functionally important regions of genes [11 ]. The biological signiﬁcance of G-quadruplexes is furtherhighlighted by their presence in the promoter regions of the c-myc [12 15 ], c-kit [16 ], ... favorsduplex formation and competes out the quadruplex[ 41] . However, to obtain a greater insight into the dynamics of the equilibrium between the folded motif and the duplex form, the G-rich sequences ... not the case for ﬂank lengths of 8 and 12 due to the presence of intramolecularstem–loop structures in both the G-rich and C-richstrands. The parameter that highlights the predominance of either...

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