Báo cáo khoa học: Identification of RNase HII from psychrotrophic bacterium, Shewanella sp SIB1 as a high-activity type RNase H pot

... doi:10.1111/j.1432-1033.2004.03980.xmolecular dynamic modelling as well as biophysical analy-ses have revealed the mechanisms that ensure very rapidtransport and at the same time high selectivity of water orglycerol transport ... is an atypical aquaglyceroporin as the highlyconserved NPA motifs in the B- and the E-loop are NPS(Asn-Pro-Ser) and NLA (Asn-Leu-Ala), respectively,sequences that are also found in the Plasmodium ... Sweden;3Swansea Clinical School, University of Wales Swansea, UK;4School of Lifeand Health Sciences, Aston University, Birmingham, UKAquaporins and aquaglyceroporins mediate the transport of waterandsolutesacrossbiologicalmembranes.Saccharo-myces...

... stop assayThis assay was adapted from the method described byHan et al. [43]. The 25-mer primer was 5¢-labeled with32P, mixed with the 76-mer template DNA and annealed as described above. The ... at 1 nM, whereas the concentration of RNase- treated RGG3 added to the bindingreaction was varied, as shown above eachlane. The equilibrium-binding curve wasobtained by calculating the fraction ... path length cell at 25 °C. The spectra of theHtelo–RGG3 complex were corrected by subtracting thespectra of the free RGG3 at the same ratios.Methylation of recombinant RGG3This assay was adapted...

... TGC TTG ATG AGC CTA CCA (atr sense) This studyatr2 TGC TGA TGA TGG CAA CTC (atr antisense) This studyasd1 AAG CCG ATG ACA CCA ATT (asd sense) This studyasd2 GCA GGT TCA TAG TGC ATG (asd antisense) ... P, Jansson PE, Rahman MM, Widmalm G,Holme T, Rahman M & Weintraub A (1994) Structuralstudies of the O-polysaccharide from the lipopolysac-charide of Moraxella (Branhamella) catarrhalisserotype ... JBiochem 265, 524–529.11 Masoud H, Perry MB & Richards JC (1994) Character-ization of the lipopolysaccharide of Moraxella catarrh-alis. Structural analysis of the lipid A from M. catarrhalis...

... AATGCTGGCTCTCCCTCGATGCTTGGCTACTGGACCATCAAHYPK GGAAATGGAAATAACAAGACAAATAGCGCGCAACTAATGCTTCCACAAHSP70 TGACCAAGGCAACAGAACCAAATCAGACGGCCGGTATGTGHeat shock 70 kDa protein 1 2A CGAAAAAGGACAGCAGTTGAAACTCATCCTCCACCGGATTGTHSP23 ... kinasecomplex-associated proteinAAAGCAGAGCAGAAAAAGTGGAAGGACAATGCCGCGATCAGNon-selenium glutathione peroxidase CAATGAACAAAAAAGTCGCAACAGGGATGGAGGGTAAGACCATACAGlutamine synthetase ACGGAGGTTGACGGGACTTGCTGGCACCACGATTGGDelta-9-desaturase ... CGTCCGATTTCTTCTCGTGTTTACCAGAAGACATTACAGTGAAAATTGAChaperonin-containing TCP1, subunit 7, isoform b, isoform 1 GGGAACCAGCAGTCGTCAAACGTCCACTGAGAGGATGAGACAInhibitor of kappa light polypeptide enhancer...

... aggregate associated with collagen in fish otolithsHidekazu Tohse1,2, Yasuaki Takagi2and Hiromichi Nagasawa11 Department of Applied Biological Chemistry, Graduate School of Agricultural and Life ... byinvertebrates, and has three crystal phases: calcite, ara-gonite and vaterite. Although calcite is the most stablecrystal thermodynamically, many organisms can formmetastable aragonite crystals ... C),heparitinase II (10 munits, H) , hyaluronidase SD (25 munits, Y) andendo -a- N-acethylagalactosaminidase (70 munits, E). The HMWaggregate was digested only by heparitinase II (arrowhead), and...

... sequence tag mining and functionalexpression assayMasaaki Shibuya1, Masaki Hoshino1, Yuji Katsube1, Hiroaki Hayashi2, Tetsuo Kushiro1, andYutaka Ebizuka11 Graduate School of Pharmaceutical ... derivatives. Bioorg Med Chem Lett7, 85–88.51 Banno N, Akihisa T, Tokuda H, Yasukawa K, Higashi-hara H, Ukiya M, Watanabe K, Kimura Y, HasegawaJ & Nishino H (2004) Triterpene acids from the ... which were shown to encode cinnamic acid 4-hy-droxylase (CYP7 3A1 1) [30], flavonoid 6-hydroxylaseHOSoyasapogenol BOHHOHOSoyasapogenol A OHHOOHHOSoyasapogenol CHOHOSoyasapogenol EHOO3242122Fig....

... inrat ovary and placenta. Cell Tissue Res 320, 525–533.45 Banan A, Zhang LJ, Shaikh M, Fields JZ, ChoudharyS, Forsyth CB, Farhadi A & Keshaarzian A (2005)Theta isoform of protein kinase ... Stratagene, La Jolla, CA,USA) and the Online Predicted Human Interaction Data-base (OPHID; http://ophid.utoronto.ca). OPHID is a web-based database of about 40 000 predicted and knownhuman protein–protein ... usingrelative quantitative real-time PCR as for Fig. 2. For each sample,b-actin was evaluated as a reference gene and used for normaliza-tion. For each gene, data are presented as the fold-increase...

... 5¢-GGAUCAAAGAGAACACUGGGGUAUA-AG-3¢;and #1513 sense, 5¢-AGUUCACGUGCAAGAACAAGUUCUG-AG-3¢. The forward sequence of the scrambledRNA was 5¢-GAUCCAAGUAAUACAGAGAUGGGAGAG-3¢. OVISE cells were plated ... assay. The main fraction contained a 75 kDamatriptase as a major component and a few contamin-ating proteins as analyzed by SDS ⁄ PAGE.RNAi experiments with OVISE cellsMatriptase siRNAs and ... Akaogi K, Okabe Y, Funahashi K, Yoshitake Y, Nish-ikawa K, Yasumitsu H, Umeda M & Miyazaki K(1994) Cell adhesion activity of a 30 kDa major secretedprotein from human bladder carcinoma...

... sequencesyef1-attB1FSD AAAAAGCAGGCTCCGAAGGAGATATAAAAATGAAAACTGATAGATTACTGyef1-attB2R AGAAAGCTGGGTGGATTGCAAAATGAGCCTGACattB1 ACAAGTTTGTACAAAAAAGCAGGCTattB2 ACCACTTTGTACAAGAAAGCTGGGTyef1hisfCAATAAATCTGCTTACGTGACATTTTTTACTAAAAGAGAATATGCGTACGCTGCAGGTCGACyef1hisrGAACCCTTGACTACGGAAACGCAGGATGTGGGAAATCGTTAATCGATGAATTCGAGCTCGpos5hisfCATAAATAAAAGGATAAAAAGGTTAAGGATACTGATTAAAATGCGTACGCTGCAGGTCGACpos5hisrCTTAGAGAATCTCATTGAATCTTTGCATTCAGAGCGTTTAATCGATGAATTCGAGCTCGpos5leu21.6fCATAAATAAAAGGATAAAAAGGTTAAGGATACTGATTAAAATGCCAATTCTGTGTTTCCCGGAAATGpos5leu21.6rCTTAGAGAATCTCATTGAATCTTTGCATTCAGAGCGTTTAGTAAAGTTCGTTTGCCGATACATGyef1up0.5kbCGTTATGAAAATCACTATTATCCCCyef1-HindIII ... ACCACTTTGTACAAGAAAGCTGGGTyef1hisfCAATAAATCTGCTTACGTGACATTTTTTACTAAAAGAGAATATGCGTACGCTGCAGGTCGACyef1hisrGAACCCTTGACTACGGAAACGCAGGATGTGGGAAATCGTTAATCGATGAATTCGAGCTCGpos5hisfCATAAATAAAAGGATAAAAAGGTTAAGGATACTGATTAAAATGCGTACGCTGCAGGTCGACpos5hisrCTTAGAGAATCTCATTGAATCTTTGCATTCAGAGCGTTTAATCGATGAATTCGAGCTCGpos5leu21.6fCATAAATAAAAGGATAAAAAGGTTAAGGATACTGATTAAAATGCCAATTCTGTGTTTCCCGGAAATGpos5leu21.6rCTTAGAGAATCTCATTGAATCTTTGCATTCAGAGCGTTTAGTAAAGTTCGTTTGCCGATACATGyef1up0.5kbCGTTATGAAAATCACTATTATCCCCyef1-HindIII ... ACCACTTTGTACAAGAAAGCTGGGTyef1hisfCAATAAATCTGCTTACGTGACATTTTTTACTAAAAGAGAATATGCGTACGCTGCAGGTCGACyef1hisrGAACCCTTGACTACGGAAACGCAGGATGTGGGAAATCGTTAATCGATGAATTCGAGCTCGpos5hisfCATAAATAAAAGGATAAAAAGGTTAAGGATACTGATTAAAATGCGTACGCTGCAGGTCGACpos5hisrCTTAGAGAATCTCATTGAATCTTTGCATTCAGAGCGTTTAATCGATGAATTCGAGCTCGpos5leu21.6fCATAAATAAAAGGATAAAAAGGTTAAGGATACTGATTAAAATGCCAATTCTGTGTTTCCCGGAAATGpos5leu21.6rCTTAGAGAATCTCATTGAATCTTTGCATTCAGAGCGTTTAGTAAAGTTCGTTTGCCGATACATGyef1up0.5kbCGTTATGAAAATCACTATTATCCCCyef1-HindIII...

... DMEM.The medium was removed 24 h after the start of transfec-tion, the monolayer rinsed twice with OptiMem, and then5 mL of was added to each flask. This was incubated for a further 16 h before harvesting ... kinetic analysis of the ACE2mutants was not feasible. These data establish animportant role for both His505 and His345 as theirreplacement results in enzyme activity being dramatic-ally reduced.Modelling ... criticalfor substrate binding such that its replacement causes enzyme activity to beabolished. Although both His505 and His345 are involved in catalysis, it isHis345 and not His505 that acts as...