Báo cáo khoa học: All-trans-retinoic acid inhibits collapsin response mediator protein-2 transcriptional activity during SH-SY5Y neuroblastoma cell differentiation pptx
Báo cáo khoa học: All-trans-retinoic acid inhibits collapsin response mediator protein-2 transcriptional activity during SH-SY5Y neuroblastoma cell differentiation pptx
... 2006 FEBS 511 All-trans-retinoic acid inhibits collapsin response mediator protein-2 transcriptional activity during SH-SY5Y neuroblastoma cell differentiation Lorena Fonta´n-Gaba´s1, Erik ... mediators isthe protein known as collapsin response mediator protein-2, which interactswith and promotes tubulin polymerization. In this study, we investigated collapsin response mediator protein-2 ... response mediator protein-2 transcriptional regulation during all-trans-retinoic acid- induced differentiation of SH-SY5Y neuroblastoma cells. All-trans-retinoic acid is considered to be a potential...
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Tài liệu Báo cáo khoa học: Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural specificity variants doc
... [32P]-tetrasodium pyrophosphate(Perkin-Elmer; specific activity in assay 5–10 cpmÆpmol)1),together with amino acid, tRNA and enzyme in 50 l L reac-tions. Radioactivity incorporated into ATP was quantifiedby ... [14C]-amino acid, tRNA and arginyl-tRNA synthetase. Amino acid incorporation was followed using 3 MM filter discs(Whatman, Dassel, Germany) that had been pretreated with50 lL 5% trichloroacetic acid ... enzymes thatrequire tRNA for amino acid activation, the potentialfor misrecognition of related amino acids has beeninvestigated [9–13] and modulated by amino acid replacements and active site...
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Tài liệu Báo cáo khoa học: Fatty acid synthesis Role of active site histidines and lysine in Cys-His-His-type b-ketoacyl-acyl carrier protein synthases ppt
... decarboxylase activity below pH 6 as does the H298Q enzyme at allinvestigated pHs, whereas the wild-type enzyme retainsa vestige of activity at pH 4 demonstrates that decarb-oxylase activity depends ... residue at position 328 is an importantfactor for efficient transacylation activity, especially at4 °C.Elongation activity of the wild-type and K328mutant KAS I proteinsThe transfer and decarboxylation ... used. This implies that, althoughK328F decarboxylation activity was equivalent to thatcharacterizing K328H and K328R, its modified trans-fer activity prohibited condensation at a level thatcould...
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Tài liệu Báo cáo khoa học: Fatty acid desaturases from the microalga Thalassiosira pseudonana pptx
... replicatedthree times.Fatty acid analysisMicroalgae or yeast cells were harvested by centrifugation.Total fatty acids were extracted and transmethylated aspreviously described [14]. Fatty acid methyl esters ... dihydroxylated substrates.AbbreviationsARA, arachidonic acid; DHA, docosahexaenoic acid; EPA, eicosapentaenoic acid; EST, expressed sequence tag; FAME, fatty acid methylester; gDNA, genomic DNA; LCB, long ... two novel microalgalenzymes involved in the conversion of the x3-fatty acid, eicosapentaenoic acid, into docosahexaenoic acid. Bio-chem J 384, 357–366.16 Meyer A, Kirsch H, Domergue F, Abbadi...
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Báo cáo khoa học: MicroRNA-9 inhibits ovarian cancer cell growth through regulation of NF-jB1 ppt
... ES-2 cells and cell growth activity was detected using a MTT assay. It wasindicated that knockdown of NF-jB1 suppressed cell growth in a time-dependent manner. A significant reduc-tion in cell ... 0.05).MiR-9 inhibits ovarian cancer cell growth L M. Guo et al.5540 FEBS Journal 276 (2009) 5537–5546 ª 2009 The Authors Journal compilation ª 2009 FEBSKnockdown of NF-jB1 inhibits ES-2 cells growthin ... 8961–8982.MiR-9 inhibits ovarian cancer cell growth L M. Guo et al.5546 FEBS Journal 276 (2009) 5537–5546 ª 2009 The Authors Journal compilation ª 2009 FEBSMicroRNA-9 inhibits ovarian cancer cell growth...
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Báo cáo khoa học: Amino acid limitation regulates the expression of genes involved in several specific biological processes through GCN2-dependent and GCN2-independent pathways ppt
... circumstances,amino acid availability regulates fatty acid homeostasisin the liver during the deprivation of an essentialamino acid [6]. These examples demonstrate thatKeywordsamino acid; GCN2; gene ... expression in response toamino acid starvation.ResultsAmino acid starvation triggers changes in geneexpressionIn order to identify amino acid- regulated genes, mouseembryonic fibroblast (MEF) cells ... amino acids exhibit two important char-acteristics: (a) nine amino acids are essential for healthin adult humans, and (b) amino acids are not stored,which means that essential amino acids...
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Báo cáo khoa học: Energetic and metabolic transient response of Saccharomyces cerevisiae to benzoic acid docx
... the food industries.Along with other weak acids such as sulfite and sulfurdioxide, sorbic acid, acetic acid, propionic acid andlactic acid, benzoic acid is used on a large scale as afood preservative, ... requirement of the cells upon suddenexposure to benzoic acid. The remaining question iswhy the cells need the energy.A possible answer is that cells need to maintainintracellular pH homeostasis ... benzoic acid influx, which isproportional to the cell surface area, the cell alsodecreases its cellular machinery which may implydecreases in metabolic flux. This would make thedecrease in cell...
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Báo cáo khoa học: Glutamic acid residues in the C-terminal extension of small heat shock protein 25 are critical for structural and functional integrity pptx
... chaperone activity with the wild-type protein. Each of theglutamic acid residue mutants displayed a decrease in chaperone activity compared with wild-type Hsp25.A. M. Morris et al. Glutamic acid ... comparable chaperone activity with the wild-type protein. Each of theglutamic acid residue mutants displayed a decrease in chaperone activity compared with wild-type Hsp25.Glutamic acid mutants of ... whereas the glutamic acid residue mutantsexhibited significantly decreased thermostability.Functional chaperone activity assays of wild-typeand mutant Hsp25The chaperone activity of wild-type...
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Báo cáo khoa học: Docosahexaenoic acid stabilizes soluble amyloid-b protofibrils and sustains amyloid-b-induced neurotoxicity in vitro ppt
... micellar detergents and fatty acids.The need for micellar structures explains why arachidic acid did not stabilize protofibrils in our study. Thecritical melting temperature for this fatty acid ... interaction of DHA with Ab have onAb-induced cell toxicity. PC12 cells, a tumor cell linederived from rat adrenal gland, were chosen as a cell model, as these cells work very well with the MTTassay ... happens when the concentration offatty acid is over 10 lm, probably due to micelle for-mation at these concentrations. It thus appears thatfatty acid micelles interact with the Ab peptide andfacilitate...
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Báo cáo khoa học: Characterization of isocitrate dehydrogenase from the green sulfur bacterium Chlorobium limicola A carbon dioxide-fixing enzyme in the reductive tricarboxylic acid cycle ppt
... IDH activity c ould bedetected in the cell- free extract after ind uction with IPTG.The activity of the recombinant cell- extract (19.5 UÆmg)1)was 100-fold higher than that in the host cells ... recombinant E. co li. IDH activity was measured with carboxylation reaction.StepTotal protein(mg)Total activity (U)Specific activity (UÆmg)1)Yield(%)Purification(fold) Cell- free extract 254 ... or reduction of unsaturated f atty acid inS. cerevisiae [29]. In contrast, the carboxylation activity o fCl-IDH at pH 7.0 was as high as the decarboxylation activity (Fig. 2 A). Cl-IDH possessed...
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