Báo cáo khoa học: Antioxidant Dps protein from the thermophilic cyanobacterium Thermosynechococcus elongatus An intrinsically stable cage-like structure endowed with enhanced stability potx
Báo cáo khoa học: Antioxidant Dps protein from the thermophilic cyanobacterium Thermosynechococcus elongatus An intrinsically stable cage-like structure endowed with enhanced stability potx
... 2006 FEBS Antioxidant Dps protein from the thermophilic cyanobacterium Thermosynechococcus elongatus An intrinsically stable cage-like structure endowed with enhanced stability Stefano Franceschini*, ... exploited during the purification of Dps proteins. To investigate Dps ther-mostability, a protein from the thermophilic cyano-bacterium Thermosynechococcus elongatus (Dps- Te)was chosen. Thermosynechococcus ... Pores at the Dps- like interface of T. elongatus Dps (A), H. salinarum Dps (B), E. coli Dps (C), L. innocua Dps (D) and M. smegmatis Dps (E). The residues forming the pores and their Van der Waal’s...
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Báo cáo khoa học: Putative prion protein from Fugu (Takifugu rubripes) ppt
... colichaperone proteins [25–27], and then purified without the use of denaturants. The proteins thus obtained werestudied with CD and NMR spectroscopy.Our results show that the putative C-terminaldomain ... purificationand refolding methods that were successful with the other species. Considering that the sequence homologyamong fish species is 60% among the PrP1 proteins,50% among the PrP2 proteins, and ... nitrogensource, the yield was 0.4 mgÆ L)1.Characterization of tr1-PrP with CD and NMRspectroscopyTo compare the conformation of tr1-PrP with that ofrecombinant mammalian prion proteins, we used CDand...
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Tài liệu Báo cáo khoa học: Fatty acid desaturases from the microalga Thalassiosira pseudonana pptx
... synthes-ized from T. pseudonana RNA using the SuperScriptTMIIIRNase H–Reverse Transcriptase (Invitrogen) or the Enhanced Avian Reverse Transcriptase (Sigma) and genespecific primers pairs ... yield the plasmids pYDESA, pYDESB, pY-DESI, pYDESO, and pYDESK. The fidelity of the clonedPCR product was checked by sequencing. The vectors con-taining the T. pseudonana sequences were then transformedinto ... and 6, and one each onchromosomes 3, 7 and 21. Material used to sequence the T. pseudonana genome was derived from a singlediploid founder and this revealed the presence of twohaplotypes with...
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Báo cáo khoa học: Small peptides derived from the Lys active fragment of the mung bean trypsin inhibitor are fully active against trypsin pptx
... induction. Lane 2: after IPTG induction for 3 h. Lane 3: the supernatant after sonication. Lane 4: the unbound fraction on the GST affinity resin. Lane 5: the purified GST–Lys33GP eluted from the GSTresin. ... of the peptide to I2) to remove the Acm protecting group and allow the formation of anotherdisulfide bond (Cys18 and Cys26) [36]. The two disulfidebonds were then correctly paired, and the ... squares,filled triangles, and filled squares, respectively.Table 1. The relative molecular masses (Mr) and inhibition con-stants (Ki) of the native MBTI, Lys33GP, the Lys16 peptide, and the mutant Lys33K...
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Báo cáo khoa học: Nitric oxide formation from the reaction of nitrite with carp and rabbit hemoglobin at intermediate oxygen saturations pdf
... reflect the faster reaction of nitrite with deoxy hemes in the R structure than in the T structure [19,20]. Thus, the reaction rate was notmaximal at the start of the reaction, where the concen-tration ... was transferred to the tonometer andequilibrated for 1 h in the shaking tonometer to ensure fullequilibration of the Hb to the gas atmosphere of the system. The tonometer was then transferred ... 2008 The Author Journal compilation ª 2008 FEBS 3381 The addition of ATP to stabilize the T state and toimpede the T to R transition caused the disappearanceof the well-defined peak for the deoxyHb...
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Báo cáo khoa học: Characterization of oligosaccharides from the chondroitin/dermatan sulfates 1 H-NMR and 13 C-NMR studies of reduced trisaccharides and hexasaccharides doc
... the characterization of these structures is therefore ofvalue.GAGs are not primary gene products and thereforetheir analysis cannot rely on genomic approaches;structural analysis requires their isolation ... for 24 h at 65 °C. The GAGs were pre-cipitated from the soluble fraction by the addition of4 vol. ethanol, and the solution was cooled to 6 °C andallowed to stand overnight. The precipitate was ... GalNAc6S(b1–4)GlcA(b1–3)GalNAc6S-ol. The full assignment of all signals in the NMRspectra are given, and these data permit the further characterization ofCS ⁄ DS chains and their nonreducing capping structures.AbbreviationsCS,...
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Báo cáo khoa học: "Mining Parenthetical Translations from the Web by Word Alignment" potx
... as the answer key. For each Chinese and English word in the Wikipedia data, we first find whether there is a translation for the word in the extracted translation pairs. The Coverage of the ... For the examples in Table 2, the words频 道(channel), 波 道 (wave passage), 信 道 (signal pas-sage), and 通道 (passage) are aligned with Channel, and the words间距(distance) and 间隔 (gap) are aligned with ... Consider the candidates for Channel Spacing in Table 2. The suffix间隔 (gap) has the highest frequency count. It is none-theless an incomplete translation of Channel Spac-ing. The correct translations...
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Báo cáo khoa học: "Semantic Class Learning from the Web with Hyponym Pattern Linkage Graphs" pdf
... whethera candidate instance is a true class member is to seewhether it can produce new candidate instances. Ifwe instantiate our pattern with the candidate (i.e.,“CLASS NAME such as CANDIDATE ... progresses. Initially, the seed is the onlytrusted class member and the only vertex in the graph. The bootstrapping process begins by instan-tiating the doubly-anchored pattern with the seedclass ... to the web and ac-quires contexts around them. The KnowItAll system(Etzioni et al., 2005) also uses hyponym patterns toextract class instances from the web and then evalu-ates them further...
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Báo cáo khoa học: "Extracting Hypernym Pairs from the Web" potx
... distance between the assignedhypernym and the target word. The ideal distance isone, which would occur if the suggested ancestor isa parent. Grandparents are associated with distancetwo and ... recall and distance. Again, the conjunctivepattern outperformed the hypernym pattern. We as-sume that the frequency of the two patterns plays an important role (the frequency of pages with the ... hierarchies from evidence collected from con-junctions. Pantel, Ravichandran and Hovy (2004)learned syntactic patterns for identifying hypernymrelations and combined these with clusters builtfrom...
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Báo cáo khoa học: Odorant binding protein has the biochemical properties of a scavenger for 4-hydroxy-2-nonenal in mammalian nasal mucosa doc
... of the protein and in particular the chemicalresistance and binding capacity with respect to HNE. The incubation time of 20 min is the time required for the clearance of a molecule from the ... residues) compared with the porcineform (8.3%) [12], and because of their spatial arrange-ment in the 3D structure of the proteins (see below). The functionality of these HNE chemically modifiedOBPs ... PUFA and lead them from the mucus present onnasal epithelia to the digestive tract for their chemicalinactivation.Results Protein purification and functionalcharacterizationSDS ⁄ PAGE of the...
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Từ khóa: Báo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018Báo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhTìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinQuản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMMÔN TRUYỀN THÔNG MARKETING TÍCH HỢPQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ