Báo cáo khoa học: Functional characterization of artemin, a ferritin homolog synthesized in Artemia embryos during encystment and diapause doc

Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt

... Meyer-Klaucke for data collection and assis-tance in data evaluation. The assistance of T. Pavkov(Institute of Chemistry, University of Graz) in theacquisition of CD and DLS data is gratefully acknowl-edged. ... metal-binding recognitionas well as reactivity in chemical transformations. Func-tional annotation of cupin proteins from sequence and 3D structural data alone is a challenging task [7], asreﬂected ... enzymatic transformationrevealed that Bxe _A2 876 catalyzed breakdown of theb-diketone substrate via oxidative carbon–carbon bondcleavage to yield methylglyoxal and acetate.Kinetic characterization...

Báo cáo khoa học: Functional characterization of front-end desaturases from trypanosomatids depicts the ﬁrst polyunsaturated fatty acid biosynthetic pathway from a parasitic protozoan ppt

... areparasitic protozoa belonging to the order Kinetoplast-ida. They are causative agents of several highly disab-ling and often fatal diseases, including African sleepingsickness, Chagas disease ... branch that D5 desaturases in nem-atodes and vertebrates may have diverged from anancestral D6 desaturase in each of these lineages aftergene duplication. L. major D6 desaturase is located in a ... was detec-ted in some protozoa, including Euglena gracilis and marine microalgae [13]. Here, C20 FAs are elongatedto C22 and then desaturated by a D4 desaturase to thesame ﬁnal 22:5 n-6 and...

Tài liệu Báo cáo khoa học: Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts pptx

... (5¢-TAATACGACTCACTATAGGGGAATTG-3¢) and reverse primer Int R (5¢-CCCATGACCTTATTACCAACCTC-3¢), respectively. The ﬁnal ampliﬁedfragment was digested with XbaI and KpnI and inserted intopAB146. ... was inserted as alinker (Figs 1 and 2).Chromosomal DNA from E. coli strain UY211 (ara,D(lac-pro), nalA, thi) [32] was isolated and used as a template in two separate PCR reactions to prepare ... (5¢-TAGTTCCCCGGGGGTGAAGCTGAAGTTTCTCTGACCGGTCAGCCGTTC-3¢) and reverseprimer tsf-DOWNS (5¢-AGTCAGGATCCGTCGACAGAGCTTCGCCACTCAACTTAAGCAGAA-3¢). Likeprimer Ts185, Ts224 contains a unique AvaI restriction site(underlined) and part of a sequence...

Tài liệu Báo cáo khoa học: Solution structure of crotamine, a Na+ channel affecting toxin from Crotalus durissus terriﬁcus venom docx

... batrachotoxin and grayanotoxin [8]. Indeed,recent studies on the inactivation kinetics of the Na+current [11] have evidenced that Crt and scorpion a- toxinsact in a very similar fashion.Based ... Catterall, W .A. (1996) Molecular determinants of high a nity binding of a- scor-pion toxin and sea anemone toxin in the S3–S4 extracellular loop in domain IV of the Na+channel a subunit. J. ... [40] and SAXS [37,38] have indicated that Crt in aqueous solution may dimerize or, more in general,aggregate. Indeed, the NMR proton spectra obtained at a protein concentration of approximately...

Tài liệu Báo cáo khoa học: Constitutive oligomerization of human D2 dopamine receptors expressed in Spodoptera frugiperda 9 (Sf9 ) and in HEK293 cells Analysis using co-immunoprecipitation and time-resolved ﬂuorescence resonance energy transfer pdf

... FLAG-taggedD2receptors, using, in this case, the following oliogo-nucleotide encoding the FLAG epitope sequence:5¢-GCGGCCGCATGGACTACAAGGACGACGATGACAAGGATCCACTGAATCTGTCCTGG-3¢.Thissequence contains, in addition ... plasmid instead of TOPO, and BaculogoldTMDNA (Pharmingen) instead of Bac-N-BlueTMDNA. All theviruses were purified using plaque assay purification and amplified by serial infection of Sf9 cells.Ó ... visualizedby means of secondary conjugated antibodies. Anti-gp120Ig and anti-FLAG Ig identified bands corresponding toproteins with a molecular mass equivalent to  43 kDa and 85 kDa for D2Land...

Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding speciﬁcity and target candidates doc

... functionugcgucugacaUGUACAGCcccugccaaauuuuaauaggcaatAGUAAAUAaauaacgacaagaagcaaauggAt5g24490 (1) Ribosomal protein; unknown function cucaucucuccuuacaguuuaccuguguaggaguuaggguucuugaauaaacaaugcaacaaagauuguagaagucagUGUACAUAAt4g36040 ... 5¢-GAGCCAACAGAAGTTTGCTTCACACGTTGTTGAGAAATGTTT-3¢ (forward) and 5¢-GTCAAACATTTCTCAACAACGTGTGAAGCAAACTTCTGTTGG-3¢ (reverse) to APUM-2 and 5¢-CGATGCAGAAATTCAGTAGCAACATGGTGGAACGATGTCTCA-3¢ (forward) ... cucaucucuccuuacaguuuaccuguguaggaguuaggguucuugaauaaacaaugcaacaaagauuguagaagucagUGUACAUAAt4g36040 (1) Protein containing DNAJ domain;unknown functioncuacgucggacggaacugggaaaccgaucaguguugguagugaguuaacucggugaccgaguuaguagaacgaguuaauuagUGUAAAUAcgaagccaAt4g39090 (1)...

Tài liệu Báo cáo khoa học: Kinetic characterization of the ﬁrst step of the ribozyme-catalyzed trans excision-splicing reaction docx

... and 300 nM (d).Observed rate constants (kobs) were obtained from these plots and are the average of two independent assays. All data points betweenthe two independent assays have a standard ... USA). Data were fit using the kaleidagraph curve-fittingprogram (Synergy Software, Reading, PA, USA). The finalconcentration of the radiolabeled substrate in all reactionsis 1.3 nm. A typical ... labeled 5¢ exon intermediate was added and the reac-tion mixture was incubated at 44 ° C for 30 min to allowcomplete binding. A chase reaction was then initiated bythe addition of 40 lL of...

Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate speciﬁcity docx

... R105H-rev:5¢-GCGAGCGCTAAAAATGGAGTGCTCCATTATTTTTAGC-3¢. R105K-fwd:5¢-GCTAAAAATAATGGAGAAATCCATTTTTAGCGCTCGC-3¢. R105K-rev:5¢-GCGAGCGCTAAAAATGGATTTCTCCATTATTTTTAGC-3¢Sequence veriﬁcationPlasmids of the ... :5¢-CTTCTTGGGATCTTTCCACATCAGCTCCAGCAG-3¢. Q81N-fwd:5¢-TGGGCCATGCCCTTTAACAGTTATGTCACGCTG-3¢. Q81N-rev:5¢-CAGCGTGACATAACTGTTAAAGGGCATGGCCCA-3¢. R105H-fwd:5¢-GCTAAAAATAATGGAGCACTCCATTTTTAGCGCTCGC-3¢ ... nucleo-side analogue can be incorporated into the DNA chain and cause chain termination, induce apoptosis or inhi-bit DNA polymerase [10,11]. Because of the high cata-lytic rate and the broad substrate...

Tài liệu Báo cáo khoa học: Spectroscopic characterization of a higher plant heme oxygenase isoform-1 from Glycine max (soybean) ) coordination structure of the heme complex and catabolism of heme docx

... thesame time, distinct absorption bands of oxyhemeappeared at 540 and 579 nm. Then, a broad bandappeared at around 660 nm, and was maximal 9–12 minafter initiation of the reaction. The spectral ... initiatesthe reaction, as revealed by gradual diminution of theSoret band. After several minutes, a broad bandappears at around 660–675 nm; this increases in inten-sity with time, indicating ... degradation rate of GmHO-1, indi-cated in Table 4, is comparable to that of SynHO-1 in the presence of NADPH, FNR, and Fd, and also tothat of rHO-1 in the presence of NADPH and CPR.Here, NADPH...

Tài liệu Báo cáo khoa học: Thermodynamic characterization of interleukin-8 monomer binding to CXCR1 receptor N-terminal domain ppt

... DASApolarare the changes in ASA of the apolar and polar residues, respectively[39]. The structure-based calculations provide a DCp of )407 calÆmol)1ÆK)1 and DASAapolar and DASApolar of )1354 and ... This approach is clearlyan approximation; according to this parameterization,the changes in heat capacity arise from binding-induced changes in the solvent polar and apolarASA. To evaluate the ... and withoutthe receptor N-domain using the vadar program[41].The DCpwas calculated using the following equa-tion:DCp¼ 0:45DASAapolarÀ 0:26 ASApolarwhere DASAapolar and DASApolarare...

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