Báo cáo khoa học: Drosophila proteins involved in metabolism of uracil-DNA possess different types of nuclear localization signals pdf

... FEBS Drosophila proteins involved in metabolism of uracil-DNA possess different types of nuclear localization signals Ga´bor Mereńyi1, Emese Końya1and Bea´ta G. Ve´rtessy1,21 Institute ... Importin a possesses two major domainsfor its adaptor function, the importin b binding (IBB)domain in its N-terminus and the C-terminal NLS-binding domain. In the absence of importin b,anauto-inhibiting ... function of cells. We identified and characterized two types of monopartite nuclear localization sequences of D. mela-nogaster proteins involved in uracil-DNA metabolism (Tables 2 and 3). The C-terminus...

Báo cáo khoa học: Sin3 is involved in cell size control at Start in Saccharomyces cerevisiae Octavian Stephan and Christian Koch ppt

... to both Swi6 and Sin3[20,21,32,34]. In fact, Stb1 was originally identified asan interacting protein of Sin3 in two-hybrid assays(Stb1 for Sin three binding) [34]. STB1 transcriptionwas shown ... is inactivated [33]. Phosphory-lation of Stb1 inhibits interaction between Swi6 andStb1 [20]. Our finding of cell-cycle-specific promoterbinding by Sin3 is in agreement with the proposal of ... or in cln3D mutants. In addition, theeffect of ectopically expressing the dominant-negativeallele of SIN3 on cell size was most obvious in cln3mutants. The importance of Sin3 for regulating...

Báo cáo khoa học: Geranylgeranyl reductase involved in the biosynthesis of archaeal membrane lipids in the hyperthermophilic archaeon Archaeoglobus fulgidus potx

... was 0.5–2 mLÆmin)1.Extraction of archaeol from H. salinaliumH. salinarum was cultured in 1 L of culture medium, con-taining 5 g of casamino acids, 5 g of yeast extract, 3 g of Archaetidic ... quantitation of microgram quantities of protein util-izing the principle of protein-dye binding. Anal Biochem72, 248–254.31 Fujii H, Koyama T & Ogura K (1982) Efﬁcient enzy-matic hydrolysis of ... mixturecontained, in a ﬁnal volume of 3 mL, 600 nmol of nonlabe-led IPP, 600 nmol of (all-E) farnesyl diphosphate, 80 lmol of a-glycerophosphate, 30 lmol of MgCl2, 300 lmol of 2-molpholinoethanesulfonic...

Báo cáo khoa học: b3-Adrenoceptor knockout in C57BL/6J mice depresses the occurrence of brown adipocytes in white fat pptx

... uncoupling protein 1.Brown adipose tissue (BAT) and white adipose tissue(WAT) both play an important role in the control of energybalance in mammals. Indeed, BAT is involved in the control of ... Ten days of cold-exposure increased the number of multilocular cellsand induced the expression of UCP1 in some of them in both WT and b3KO mice.Mice maintained singly housed at 24 °C are ... It is interesting to note in the WAT of C57mice at 24 °C a signiﬁcant level of expression of UCP1mRNA reaching 3% of that observed in BAT. Our studyshows for the ﬁrst time that the lack of b3-adrenoceptorstrongly...

Tài liệu Báo cáo khoa học: Parvalbumin deficiency in fast-twitch muscles leads to increased ‘slow-twitch type’ mitochondria, but does not affect the expression of fiber specific proteins doc

... Schiffmann SN (2002) ‘New’functions for ‘old’ proteins: The role of the calcium-binding proteins calbindin D-28k, calretinin and parval-bumin, in cerebellar physiology. Studies with knockoutmice. ... and Ca2+ﬂuxes [8]. An increasedbiogenesis of mitochondria was detected in skeletalmuscle of null-mutant mice for proteins involved in ATP metabolism, such as creatine kinase [9] and theADP ... RT-PCRresults of increased PCR signals in PV– ⁄ – TA. Thetwofold increase also observed in CaN activity sup-ports a prominent role for the CaN-mediated pathway in this process. Two lines of evidence indicate...

Tài liệu Báo cáo khoa học: Altered deoxyribonucleotide pools in T-lymphoblastoid cells expressing the multisubstrate nucleoside kinase of Drosophila melanogaster doc

... 5-ﬂuoro-2¢-deoxyuridine;5-I-dUrd, 5-iodo-2¢-deoxyuridine; ACV, acyclovir; araC, 1-b-D-arabinofuranosylcytosine; araG, 9-b-D-arabinofuranosylguanine; araT, 1-b-D-arabin-ofuranosylthymine; BVaraU, ... caused by mutations in the nuclear gene for thymidine phosphorylase, which results in increased levels of thymidine. This enzyme catalyzes thephosphorolysis of thymidine to thymine and deoxyribose1-phosphate, ... with disorders in nucleotide metabolism. The increased incorporation of dThd, dCyd andBVDU in DNA of the Dm-dNK gene-transfected cellsAltered dNTP pools in T-cell lines expressing Dm-dNK A....

Báo cáo khoa học: Starch-binding domains in the CBM45 family – low-afﬁnity domains from glucan, water dikinase and a-amylase involved in plastidial starch metabolism pptx

... starch-bindingability of AtAMY3 in vitro. It cannot be precludedthat some binding may involve secondary binding sites in the catalytic domain, but the demonstrated starch-binding ability of different ... as a starch-binding domain(SBD) [3].CBMs are noncatalytic structural domains thatmediate the binding of proteins to polysaccharides,thus bringing the appended catalytic modules intoclose contact ... b-cyclodextrin injection, and thebottom panel depicts the binding isotherm; open circles representthe integrated binding heat of the data in the top panel, and the fullline is the ﬁt of a one-site binding...

Báo cáo khoa học: Short hydrogen bonds in proteins Sathyapriya Rajagopal and Saraswathi Vishveshwara pot

... SHBs in general are found to be involved in stabilizing the three dimensional structures of proteins at specific structural locations as discussedabove. A single protein such as copper amine oxidaseAsp ... Hydrogenbonds in the alpha helices of globular proteins. Biophy-sics 46, 969–977.35 Wilmot CM & Thornton JM (1988) Analysis and pre-diction of the different types of beta-turn in proteins. J ... statistics of SHBs in proteins Using the criteria specified in the Experimental proce-dures section, SHBs have been identified from the data-set of 948 proteins, after fixing hydrogen atoms usingboth...

Báo cáo khoa học: LmbE proteins from Bacillus cereus are de-N-acetylases with broad substrate speciﬁcity and are highly similar to proteins in Bacillus anthracis pot

... presence of three protein bands. The position of the main band is in agreement with the predicted molecular mass of thehexahistidine fusion protein (27 kDa). The N-terminalamino acid sequence of ... tuberculosis H37RvFig. 6. Neighbor joining tree for 929 LmbE proteins. Bacillus cereus proteins and pro-teins of known function are indicated.A. Deli et al. LmbE proteins from Bacillus cereusFEBS ... Bacillus proteins were placed in func-tional categories different from other LmbE proteins. As the sequence of the protein is not sufﬁcient toreveal the function of the enzyme, we sought otherlines...

Báo cáo khoa học: Autoregulatory binding sites in the zebraﬁsh six3a promoter region deﬁne a new recognition sequence for Six3 proteins potx

... identical to the corresponding DNA-bindingdomain of orthologous Six3 proteins in other verte-brates, displayed differential binding affinities for vari-ous sites containing a TAAT core motif, and ... specifically favouring binding toSix3 proteins were not identified.Our examination of the interaction of zebrafishSix3a with 43 TAAT-containing sequences, distributedwithin the promoter region of its own ... comparisons of the binding affinities of the 18 different ATTA-containing sites. In this study,we used a biotin-labelled 27 bp DNA fragment con-taining a1 as a probe in EMSAs, and tested the in u-ence...

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