Evaluation of in-house PCR for diagnosis of smear-negative pulmonary tuberculosis in Kampala, Uganda doc

... evaluated for the diagnosis of smear-negative PTB in the same setting.Using LJ culture as the base-line test, this study evalu-ated in- house PCR for rapid diagnosis of smear-negative PTB in a low income ... RES E A R C H A R T I C L E Open Access Evaluation of in- house PCR for diagnosis of smear-negative pulmonary tuberculosis in Kampala, Uganda Lydia Nakiyingi1,2*†, David P Kateete3†, Ponsiano ... was 0.7 [95% CI(0.43-1.14)] implying that a negative in- hou se PCR testis not indicative of absence of MTBC. Therefore, with in- house PCR in this setting, a clinician will need add-itional diagnostic...

Tài liệu Association of killer cell immunoglobulin-like receptors with pulmonary tuberculosis in Chinese Han pdf

... response in the remaining individuals only succeeds in containing the infection, as some bacilli escape killing by the mechanisms of im-mune activity and remain in latent or dormant states. Clinically, ... sets of primers were designed for each KIR gene (except for 2DS5; Table 1). PCR was preformed within a 20-μL system containing 6.6 μL PCR loading dye mix (Takara, Kyoto, Japan) including Taq ... temperature of 65°C for 30 s. Subsequently, 20 cycles were performed at a melting temperature of 94°C for 30 s, an an-nealing temperature of 62°C for 30 s, and an extension temperature of 72°C for...

Diagnostic and Therapeutic Problems of Pulmonary Tuberculosis in Elderly Patients doc

... likelyto die of the disease (6, 14). Age-related changes in the tuber-culin skin reaction and a high incidence of underlying illnessesalso play a role in prolonging the final diagnosis (15). ... of Internal Medicine; Department of Radiology*, Seoul National University College of Medicine, Seoul; Department of Radiology, AsanMedical Center, University of Ulsan College of Medicine,Seoul, ... diagnosis of TB in the elderly. A positive spu-tum AFB smear was obtained in 57.1% of elderly and in 57.5% of young TB patients, and a positive AFB culture was ob-tained in 70.6% of the elderly...

Báo cáo y học: "MALDI-TOF MS Combined With Magnetic Beads for Detecting Serum Protein Biomarkers and Establishment of Boosting Decision Tree Model for Diagnosis of Colorectal Cancer"

... was performed using an all -in- one peptide reference standard which contained vasopressin (1084.2Da), somatostatin (1637.9Da), bo-vine insulin β chain (3495.9 Da), human insulin re-combinant ... patterns for the diagnosis of CRC, the intensities of the protein peaks in the training set were submitted to BPS. A total of four peaks (2870.7, 3084, 9180.5, 13748.8) with the highest discriminatory ... useful tool for integrating separation and analysis of complex mixtures of proteins. Captured proteins are then analyzed by TOF-MS, generating a spectral map depicting approximations of the molecular...

Evaluation of Demonstration Test Results of Alternative Technologies for Demilitarization of Assembled Chemical Weapons A Supplemental Review for Demonstration II pptx

... short itsfact-finding efforts for input to this report as of March 30,2001. This cutoff was necessary in order to provide thesponsor with the needed information in a timely fashion. In 1996 the ... Cutting and Washout, 31Shredding of Metal Parts and Dunnage, 32SET™ Treatment of Shredded Metal Parts and Dunnage, 32Reevaluation of Steps Required for Implementation, 33Reevaluation of Findings ... problems of corrosionand plugging.Finding DII FEK-11. The experience of multiple shutdownsduring Demo II testing of the TW-SCWO and the resultingthermal stresses and crack generation in the liner...

Evaluation of Demonstration Test Results of Alternative Technologies for Demilitarization of Assembled Chemical Weapons A Supplemental Review docx

... Committee Findings, 24Supplemental Findings and Recommendation, 245 UPDATE OF GENERAL FINDINGS AND RECOMMENDATIONS 26Review of Earlier Findings and Recommendations, 26Supplemental General Findings, ... solid materials of interest. In the initialreport, the committee had stated the following (NRC, 1999):Finding GA-4. Shredding of dunnage and injection of the slurry directly into a SCWO system ... by-products in otherparts of a full-scale system.The committee’s earlier report contained the followingfinding concerning hydrolysis of energetics (NRC, 1999):Finding GA-2. Hydrolysis of energetics...

Evaluation of a diagnostic algorithm for smear-negative pulmonary tuberculosis in HIV-infected adults doc

... be initiated. Clinicians working in Khayelitsha found that difficulty making the diagnosis of TB in HIV-infected people when following these protocols led to delays in the initiation of TB ... clinical algorithm used for the diagnosis of smear-negative pulmonary tuberculosis (SNPTB) in HIV-infected adults. Design. An algorithm was designed to facilitate clinico-radiological diagnosis ... method for making an early diagnosis of PTB. The reduction in sensitivity of this test in HIV-infected patients leads to diagnostic delay. Sputum culture is a more sensitive method of diagnosing...

Commercial Serodiagnostic Tests for Diagnosis of Tuberculosis: Policy Statement ppt

... laboratory-based evaluation of 19 commercially available rapid diagnostic tests for tuberculosis 3 2.1.3 Case study of economic and epidemiological impact of serologic testing for active tuberculosis in India ... rapid diagnosis of TB and in a suitable format (e.g. point -of- care) would be particularly useful both as a replacement for laboratory-based tests and for extending TB diagnosis to lower levels of ... non-commercial tests for pulmonary TB (254 datasets including 51 distinct single antigens and 30 distinct multiple-antigen combinations) identified potential candidate antigens for inclusion in an antibody...

Tài liệu Diagnosis of smear-negative pulmonary tuberculosis in people with HIV infection or AIDS in resource-constrained settings: informing urgent policy changes docx

... 2007 Diagnosis of smear-negative pulmonary tuberculosis in people with HIV infection or AIDS in resource-constrained settings: informing urgent policy changesHaileyesus Getahun, Mark Harrington, ... algorithm for the diagnosis of smear-negative pulmonary tuberculosis included in their national tuberculosis control and treatment guidelines. The fi ndings were confi rmed and complemented by interviews ... programmes for the diagnosis of smear-negative pulmonary tuberculosis of both adults and children with HIV infection. We also identify diffi culties and ways to improve the diagnosis of smear-negative...

Tài liệu Handbook of Ecological Indicators for Assessment of Ecosystem Health ppt

... InformationFrom a more holistic point of view, some authors proposed indices capable of integrating the whole environmental information. An appro ach for application in coastal areas was first developed ... applied in stations M3 (duri ng spring), M4 and M2. In the otherstations, the absence of amphipods originated values tended toward infinity,therefore had no meaning.Table 3.12 Values obtained ... as in the case of the Mondegoestuary, and in detecting organic enrichment situations, as in the case of theMar Menor marine lagoon. In fact, the more complex Shannon–Wiennerindex has been influenced...

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