Báo cáo khoa học: The mouse Muc5b mucin gene is transcriptionally regulated by thyroid transcription factor-1 (TTF-1) and GATA-6 transcription factors pdf

... French governments works The mouse Muc5b mucin gene is transcriptionally regulated by thyroid transcription factor-1 (TTF-1) and GATA-6 transcription factors Nicolas Jonckheere1,2, Ame´lie ... Cancer86, 162–168.11 Escande F, Porchet N, Aubert JP & Buisine MP (2002) The mouse Muc5b mucin gene: cDNA and genomicRegulation of Muc5b mucin gene by TTF-1 and GATA factors N. Jonckheere ... for these factors in both the human [29] and murine (this report) MUC5B mucin genes, a restricted pattern of MUC5B expres-sion in the respiratory tract [5] and the expression of MUC5B, TTF-1 and...

Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc

... in the control of gene expression. Aseach step of the RNA metabolism is tightly regulated, the regulation of mRNA export, stability and transla-tion rate is essential for the control of the ... lacking this motif(DRS1) accumulated in the cytoplasm. By contrast,ASF ⁄ SF2 nucleo-cytoplasmic distribution is modiﬁedneither by the removal of the RS2 sub-domain, nor by point mutations disrupting ... was isolated and analyzed by northern blot using Fluc and Rluc riboprobes. The radioactive signals were quantiﬁed by the STORM 820 equipment and IMAGEQUANT software (Molecular Dynamics). The...

Tài liệu Báo cáo khoa học: The Pseudomonas aeruginosa nirE gene encodes the S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase required for heme d1 biosynthesis doc

... heme d1biosynthesis genes and the co-production of both NirE and the other hemed1biosynthesis proteins, in high amounts ensures the efﬁcient and highly concerted action of the proteins. Inorder ... proteins – NirE-His, Trx-S-NirE-His and S-NirE-His – and compared their catalytic activities. Weobserved that Trx-S-NirE-His and S-NirE-His showedsimilar activities. By contrast, NirE-His showed ... phase material and analysed by UV-visible absorption spectroscopy and mass spectrometry. In both cases (NirE-His and Trx-S-NirE-His production) the UV-visible absorptionspectrum of the extracted...

Tài liệu Báo cáo khoa học: The unique sites in SulA protein preferentially cleaved by ATP-dependent Lon protease from Escherichia coli ppt

... that the activity of the enzymewas inhibited by a low concentration of SDS (about 50%inhibition in the presence of 0.0025% SDS). On the otherhand, an extensive degradation of SulA3±169 by the ... of incubation. On the other hand, nodegradation of MBP was observed either in the presence orin the absence of ATP under the conditions used. Theseresults indicated that the enzyme speci®cally ... SulAmight a lso e xist in other e nterobacteria. It is a lso aninteresting issue to see whether such a mechanism exists inother proteolytic regulatory systems, such as the protea-some±ubiquitin...

Tài liệu Báo cáo khoa học: The crystal structure of coenzyme B12-dependent glycerol dehydratase in complex with cobalamin and propane-1,2-diol pptx

... performed, and the monitor and the packing values were checked to determine the result from the candidates. We concluded that there is an (abc)2dimer in an asymmetric unit of the cell. The calculated ... dehydratase is a keyenzyme for the dihydroxyacetone (DHA) pathway[23,26,27], and its genes are located in the DHA regulon[28,29]. On the other hand, diol dehydratase is a key enzymefor the anaerobic ... thatwith the (S)-isomer (Table 3). The afﬁnity of the enzyme for the (S)-isomer was essentially the same or only slightlyhigher than that for the (R)-isomer [Km(R)/Km(S)¼ 1.5]. Thispreference...

Báo cáo khoa học: The SWI⁄SNF protein BAF60b is ubiquitinated through a signalling process involving Rac GTPase and the RING ﬁnger protein Unkempt doc

... withmultiple transcription factors and are thought tobridge interactions between these transcription factors and SWI ⁄ SNF complexes, thereby allowing the recruit-ment of SWI ⁄ SNF to target genes ... factors (BAF). Among the later, the 60 kDa subunit BAF60 is found in most complexes; it can be represented by BAF60a, BAF60b or BAF60c, which are encoded by the smarcd1, smarcd2 and smarcd3 genes, ... impair the binding of RacGTP. The data indicate that Unkempt is a speciﬁc partnerof the GTP-bound form of RacGTPases and thereforesuggest that its function, whatever it is, may be regulated by...

Báo cáo khoa học: The capsid protein of human immunodeﬁciency virus: interactions of HIV-1 capsid with host protein factors ppt

... one in the center of the hexameric ring and another in a trilobed hole ﬂanked by the hexamer spokes [72].Although the mechanism of restriction is stillunclear, reverse transcription of the viral ... structural changes in the CA-CTD, as is evident by the inability to form aC198-C218 disulﬁde bond, which is surprising consid-ering that87HAGPIA92 is located in the CA-NTD[46]. The latter result ... increase was minimal. This supports the hypothesisthat TRIM5a causes premature uncoating ⁄ disassemblyof the viral capsid, which is detrimental to reverse transcription [70] and suggests an interaction...

Báo cáo khoa học: The Vps4 C-terminal helix is a critical determinant for assembly and ATPase activity and has elements conserved in other members of the meiotic clade of AAA ATPases pdf

... important. One of these, the TRP sequence, includes the start of the C-terminalhelix as well as the structured loop between the ATPase domain and the C-terminal helix and in the 3D structure is positioned ... helix). However, the majority of these proteins are likely to be other meioticclade AAA ATPases and have the AAA domain helix and the C-terminal helix, but not the b domain. The distinguishing feature ... sequence is positioned very close to the ATP-binding pocket in the adjacent subunit. Thus the TRP sequence at the beginning of the C-terminal helix and the RDFsequence at the end of the C-terminal...

Báo cáo khoa học: The role of interface framework residues in determining antibody VH ⁄ VL interaction strength and antigen-binding afﬁnity pptx

... verification of the hypothe-sis is needed. However, this will not be difficult if the spFv system is utilized.In the present study, we demonstrated that libraryscreening is indeed possible using the spFv ... sequences of all the mutants and the wild-type, sorted by this index, are summarized in Table 1.It is worth noting that while two clones with low affin-ity (1D5 and 4F1) had W at H47, the other 34 cloneshad ... affinity and VH⁄ VLinteraction strength, anovel phage display system that can switch two displaymodes, named the split Fv (spFv) system, wasemployed. The spFv system can either display VH and VLfragments...

Báo cáo khoa học: Pleiotropy of leptin receptor signalling is deﬁned by distinct roles of the intracellular tyrosines docx

... lg each of the luciferase reporter construct and the b-galactosidase reporter control plasmid pSVb-gal(Promega). Transcription of the lacZ gene in this controlvector is driven by the SV40 early ... leptin-induced activation of STAT factors wasmonitored by Western blot analysis with phospho-spe-ciﬁc antibodies and by reporter gene assays (Fig. 4).Consistent with the established consensus sequence ... whereas the glutamine in position P +3 is required for binding ofSTAT1 and STAT3, but is not relevant for STAT5. Thisresult is consistent with the fact that residues C-terminalof the phosphotyrosine...

Xem thêm