... Identificationof a twin-arginine leader-binding protein. Mol. Microbiol. 40,323–331.14. Papish, A.L., Ladne r, C.L. & Turner, R .J. (2003) T he twin-arginine leade r-binding protein, DmsD, interacts ... withpuromycin or puromycin in a Ôhigh saltÕ buffer aftertranslation but prior to c rosslinking. Both t reatmentsdiminished crosslinking to TF (Fig. 1B, lane 5 a nd 6),indicating that association ... UV-light to inducecrosslinking or kept in th e dark as indicated. UV-irradiated ribosome-nascent chain complexes were immunoprecipitated (IP) with TF a ntiserum asindicated. Prior to crosslinking,...