... explained by the association of these proteins in acomplex. As in the preceding fractionations, eluted proteinswere analyzed by SDS/PAGE, followed by Coomassie bluestaining. At this stage of the ... S100A8/A9,accompanied by a majority of p67phox, p47phox and Rac.Analysis of the protein distribution in the two HTP pools by SDS/PAGE followed by Coomassie blue staining revealed adiscrete number of proteins, including ... traces of protein contaminant [15]. The identity of S100A8 was furtherascertained by amino-acid sequencing, using Edman degra-dation. As S100A9 has a blocked N-terminal amino acid,analysis of the...