Báo cáo khoa học: Inhibition of the NF-jB transcriptional activity by protein kinase A pot
Báo cáo khoa học: Inhibition of the mitochondrial calcium uniporter by the oxo-bridged dinuclear ruthenium amine complex (Ru360) prevents from irreversible injury in postischemic rat heart docx
... [Ca2+]minisolated hearts was to measure the activated pyruvatedehydrogenase (PDH) activity in heart homogenates at the end of the perfusion protocols. PDH is activated by a calcium-dependent phosphatase. ... mitochondria obtained fromuntreated hearts; (m) values from mitochondria obtained fromhearts treated with Ru360. Each value was obtained from a singleheart and the data represent the mean ± SE of at ... biochemical populations of cardiac mito-chondria may explain this observation. It has beenreported that subsarcolemmal mitochondria (SLM) arelocated beneath the plasmatic membrane and thatinterfribillar...
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Báo cáo khoa học: Inhibition of the NF-jB transcriptional activity by protein kinase A pot
... 1E and F). These data indicate thatelevation of intracellular cAMP and subsequent activation of PKA lead to the inhibition of NF-jB activity. To further examine the effect of PKA on NF-jB activity, we ... Inhibition of the NF-jB transcriptional activity by protein kinase A Naoko Takahashi, Toshifumi Tetsuka, Hiroaki Uranishi and Takashi OkamotoDepartment of Molecular Genetics, Nagoya City ... examined whether cAMP and PKAmodulates NF-jB activity and found that the cAMP/PKAsignaling pathway inhibits the NF-jB activity by acting on the transactivation domain of p65. Possible mechanisms...
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Báo cáo khoa học: Inhibition of the D-alanine:D-alanyl carrier protein ligase from Bacillus subtilis increases the bacterium’s susceptibility to antibiotics that target the cell wall potx
... cassette was con-firmed by PCR.dLtA-P1, 5¢-ACAAATATAGACACCGAGCAAAATGGCAA; dLtA-P2, 5¢-CGAGCTCGAATTCGTAATCATGGTCATATTATAAATATATGAACCGCTATTCGCGGT-3¢(3¢ kanamycin fragment underlined); dLtA-P3, ... no A- domain with a d-aa as the sole substrate has beenbiochemically characterized. Especially the fact that the enzyme does not activate l-Ala corroborates the finding that A- domains as well as aaRS ... 5¢-GATACCATGGAACTTTTACATGCTATTCAAACAC-3¢ and 5¢-GATAAGATCTTACAAGAACCTCTTCGCCAATG-3¢ from chromosomalDNA of B. subtilis ATCC21332 and, after restriction digest of the amplified fragment, ligated into the NcoI and BglIIsites...
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Tài liệu Báo cáo khoa học: Inhibition of cobalamin-dependent methionine synthase by substituted benzo-fused heterocycles pptx
... using the Protein Assay kitbased on the method of Bradford [44]. Standards and sam-ples were assayed in triplicate, according to the manufac-turer’s instructions. Sample absorbances were read against a ... and the variable substrates. Plausible rationalizationincludes the possibility that the relatively small nitro-benzothiadiazole may displace the dimethylbenzimidaz-ole side chain of the cobalamin-cofactor ... min, the reaction wasinitiated by the addition of 500 lm (dl)-homocysteine andincubated at 37 °C for a further 30 min, unless otherwisestated. The reaction was terminated by the addition of...
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Tài liệu Báo cáo khoa học: Regulation of the actin–myosin interaction by titin doc
... actin-activated myosinATPase activity in the same way as T800 [64,69]. Inhibition of the ATPase activity was then explained by a slowingdown of the formation of active complex in solution. On the other ... we measured the Mg2+-ATPase activity of HMM and various actin–HMM complexes in the presenceor absence of T800. The ATPase activity of HMM alonewas not changed i n the presence of T800 (varying ... measured at 25 °C. The reaction was started by the addition of 2 mMATP andstopped after 10 min by 5% trichloroacetic acid. The amount of Piliberated was evaluated c olorimetrically [46].The...
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Tài liệu Báo cáo khoa học: Inhibition of glyceraldehyde-3-phosphate dehydrogenase by peptide and protein peroxides generated by singlet oxygen attack docx
... singH2O2. The role of radical vs. nonradical processes has beeninvestigated, as has the prevention of such damage.MATERIALS AND METHODSAmino acids, peptides and antioxidants were commercialsamples ... can also arise via radicalTable 2. Percentage of enzyme activity retained after incubation of GAPDH, GR and LDH with N-Ac-Trp-OMe peroxides or H2O2at 37 °Cintheabsence, or presence, of a ... that thiols can ameliorateinactivation of GAPDH induced by these1O2-generatedpeptide and protein peroxides, presumably by acting assacrificial targets. This is in accord with the known rapiddepletion...
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Báo cáo khoa học: Characterization of the rice carotenoid cleavage dioxygenase 1 reveals a novel route for geranial biosynthesis ppt
... 3-OH-c-car-otene and lycopene. The values represent ratios of the C17and C19dialdehydes in the sum of their peak areas calculated by integratingeach peak at its individual kmax. Data represent ... citral, the mixture of geranial and its cis-isomer neral, is a major component of the aroma of lemon grass and other lemon-scentedplants. Geranial is synthesized from geranyl diphos-phate by the ... localization and induction by highlight. MolMicrobiol 69, 231–244.41 Prado-Cabrero A, Estrada AF, Al-Babili S & Avalos J(2007) Identification and biochemical characterization of a novel carotenoid...
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Báo cáo khoa học: Inhibition of recombinant human maltase glucoamylase by salacinol and derivatives pdf
... FEBSstarch digestion. SIM accounts for almost all sucrase activity, all isomaltase activity, and 80% of the maltase activity, while MGA accounts for all glucoamylase activity, 20% of the maltase activity, ... four-carbon chain of salacinol and the seven-carbon chain of kotalanol.Fig. 2. Schematic diagram of MGA protein organization and expression construct.Amino acid boundaries of each of the domains ... of the breakdown of maltose, 5 lm acarbose onlyinhibited 4% of the activity. Thus, it would appear thatacarbose acts mainly by inhibiting human pancreatic a- amylase (HPA) and the breakdown of...
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Báo cáo Y học: Inhibition of the SERCA Ca21 pumps by curcumin Curcumin putatively stabilizes the interaction between the nucleotide-binding and phosphorylation domains in the absence of ATP pot
... presence of curcumin, using the coupledenzyme assay. In Fig. 3A, the half-maximal activation of the ATPase by Ca2þwas measured in the absence of and in the presence of 10 and 25 mM curcumin. The ... of Ca2þ(Fig. 4A) . The data showed that the amount of ATPbound to the ATPase was reduced by curcumin. The binding inhibition had a apparent Ki(IC50) of about 9 mM. Reversing the order of ... occur as at pH 6 the ATPase is essentially all inan E2 conformation (high fluorescence state) while the Fig. 3. The effects of Ca21and ATP dependence of Ca21ATPase activity by curcumin. (A) Ca2þ-ATPase...
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Báo cáo khoa học: Modulation of the Arabidopsis KAT1 channel by an activator of protein kinase C in Xenopus laevis oocytes potx
... calcium-dependentphosphorylation at some of the pinpointed residues in the cytosolic region of KAT1.AbbreviationsAAPK ⁄ ABR kinase, ABA-activated protein kinase ⁄ ABA-responsive kinase; ABA, ... phosphorylation assay, the Vicia AAPK ⁄ ABR kinase has been shown to phos-phorylate the C-terminal region of KAT1 [16]. One of the 10 members of the SNF1-related protein kinase 2in A. thaliana, SnRK2.6, ... activation. (A) Change in the current amplitudes of the quintuple KAT1 mutantKAT1-T30 8A- S31 2A- S58 9A- S59 0A- S64 1A after PMA application. The characteristics of WT KAT1 in the presence and absence of PMA...
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Từ khóa: chuyên đề điện xoay chiều theo dạngNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ