Báo cáo khoa học: X-ray crystallography, CD and kinetic studies revealed the essence of the abnormal behaviors of the cytochrome b5 Phe35fiTyr mutant pdf
Báo cáo khoa học: X-ray crystallography, CD and kinetic studies revealed the essence of the abnormal behaviors of the cytochrome b5 Phe35fiTyr mutant pdf
... chainconformation of this residue. The shift of the Ca atom of Tyr35 of the Phe35fiTyr mutant from that of Phe35 of the wild-type cyt b5is 0.21 A˚, within the error limit. The sidechain of Tyr35 of the Phe35fiTyr ... other mutants.However, the conformation of the latter is flexible. CD spectra of thermal denaturation of cytb5 and its Phe35fiTyr mutant Fig. 3A shows the CD spectra of wild-type cyt b5in the ... [35,36,40,41]. The apo-cyt b5 and apo-Mb have no absorption in the Soret bandregion because of the lack of the heme prosthetic group. For the holo-cyt b5 and holo-Mb, the CD spectra of the Soretband...
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Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx
... from low amounts of pantoate [9]. Here, wereport the results of the structural studies of the panto-ate-bound form of the N-terminal domain of E. coliPS (nPS). The structure and substrate-binding ... molecule takes the position of the adenine ring of ATP. The carbon and oxygen atoms of pantoate are shown inyellow and red, respectively. The carbon and oxygen atoms of ATP are shown in red, the nitrogen ... second molecule of pantoate binds to the pantoyl adenylate-bound form of the protein, at a site other than the active site,which then has a regulatory role in the kinetics of the reaction. Using...
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Báo cáo khoa học: Catalytic residues Lys197 and Arg199 ofBacillus subtilis phosphoribosyl diphosphate synthase Alanine-scanning mutagenesis of the flexible catalytic loop ppt
... Crystallization of the enzyme in the presence of the transition state analogue AlF3, the substrate Rib5P and the product AMP resulted in astructure with a bend arrangement of AMP-AlF3-AMP and with Rib5P ... interaction of hydrophobic aminoacids of b-strands located on either side of the flexiblecatalytic loop. The tightly packed interface prevents the closure of the loop, and thus, prevents the interaction of ... KRib5Pvalues of the mutant enzymeswere much less altered, if at all, compared to those of the wildtype enzyme. The Ki(ATP)value of the K197A mutant was reduced six-fold compared to that of the wildtype...
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Báo cáo khoa học: Crystal structure determination and inhibition studies of a novel xylanase and a-amylase inhibitor protein (XAIP) from Scadoxus multiflorus pot
... surfaces of the protein. Com-parison of the structure of XAIP with that of xylanase inhibitor protein-I, and docking studies, suggest that loops a3–b4 and a4 b5 may be involvedin the binding of GH11 ... from the protein surface into the solvent. There is yetanother interesting feature of the XAIP structurewhich is related to the conformation of loop b3–a3.This loop extends via the centre of the ... at the centre of the b-barrel,thus reducing the internal space of the TIM barrelconsiderably. Of the three observed cis peptides, two(Gly33–Phe34 and Trp253–Asp254) are found at the ends of...
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Báo cáo khoa học: Structural flexibility in Trypanosoma brucei enolase revealed by X-ray crystallography and molecular dynamics pdf
... the coordinated loop dimension(EV1) and the His156-out dimension (EV2). The tra-jectory reaches the EV1 value of the open sulfate_1*structure (maximum 1.73) and exceeds the EV2 values of the ... carboxylate oxygen of the substrate bridges the magnesium ions at the active site of enolase: struc-ture of the yeast enzyme complexed with the equilibriummixture of 2-phosphoglycerate and phosphoenolpyr-uvate ... labelled. Note the gaps in loops 1 and 3 of the sulfate_1 structure and the loop 3 gap in the sulfate_2 struc-ture. Side chains of Lys155 and His156 are shown as sticks, as are the structures’...
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Tài liệu Báo cáo khoa học: Hypothalamic malonyl-CoA and CPT1c in the treatment of obesity pptx
... Many of these stud-ies rely heavily on inhibitors that may affect the newlyidentified and structurally similar CPT1c. Therefore,some of these studies need to be re-evaluated in light of the ... ghrelin and adiponectin alter hypothalamic AMPK and malo-nyl-CoA levels [13,23,28,30–33]. The genetic evidencefor the role of AMPK in the hypothalamus is less clearbecause the loss of the AMPKa2 ... lipid nutrient sensing in the brain and is an example of an enzyme that can sense and respond to the nutritional environment. The majorchallenge to understanding the role of CPT1c is todetermine...
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Tài liệu Báo cáo khoa học: Activation loop 3 and the 170 loop interact in the active conformation of coagulation factor VIIa pdf
... 158(21) and 296(154) [14–17]. The high specific activity of theseFVIIa variants is presumably linked to a more stableburial of the N-terminus of the protease domain in the activation pocket of the ... included in the pub-lished alanine scanning mutagenesis study of FVIIa[13], and investigated the effects of the mutation on the enzymatic maturation of FVIIa and on the response of FVIIa to ... cleavage of the scissile bond. This would affect FX and peptidehydrolysis similarly, supported by the similar decreasein the rate of cleavage of both types of substrates. The effect of the G372(223)A...
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Tài liệu Báo cáo khoa học: Toggle switches, pulses and oscillations are intrinsic properties of the Src activation/deactivation cycle doc
... presenting the pulse of Src activity in the plane of the inactive and active fractions, si and s1. If the duration of the stimulus exceeds the critical value, the trajectory in the (si, ... conformation, the SH2 domain binds to pYion the C-terminal tail, and the Src homology 3 (SH3) domain binds to the linkerbetween the SH2 and kinase domains at the back of the small lobe, preventing the ... each of the six kinetic parameters involved(see, Fig. S3). We found that, when the ratio d of the catalytic efficiencies of the partially and fully activeforms (S and Sa1) is too large, the...
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Tài liệu Báo cáo khoa học: SREBPs: protein interaction and SREBPs Ryuichiro Sato doc
... Sp1-binding sites, and all of these sites arerequired for full activation of the LDL receptor pro-moter. Similarly, a number of promoters of the SREBP target genes contain the SREs and certainproximal ... that the nuclear importoccurs in the absence of importin-a. Furthermore, the dimerization of SREBPs via the leucine zipper domainis required for the interaction with importin-b [6]. In the nucleus, ... structure.With the increase in the number of proteins modifiedby SUMO, it has become obvious that the effects of SUMO conjugation are diverse and largely depend on the function of the protein targeted...
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Tài liệu Báo cáo khoa học: Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt
... resulted in 55% and 51%loss of the initial activities of BHLmL2 and BHAb,respectively. These results strongly suggest that Ser isinvolved in the catalytic activity of BHLmL2 and BHAb and are consistent ... 97% inhibition of BHLmL2 and BHAb, respectively. The influence of pH on the activities of the BHenzymes was investigated in the pH range 6–11. The pH optima were determined to be in the basic range(pH ... and BHAb. The temperature dependence of the activities of the BHs was tested in the range 5–56 °C; the apparentoptimum temperatures of BHLmL2 were 25–30 °CTable 3. Masses and scores of tryptic...
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