Báo cáo khoa học: Epoxidation of benzo[a]pyrene-7,8-dihydrodiol by human CYP1A1 in reconstituted membranes Effects of charge and nonbilayer phase propensity of the membrane pot

... PRIORITY PAPER Epoxidation of benzo[a]pyrene-7,8-dihydrodiol by human CYP1A1 in reconstituted membranes Effects of charge and nonbilayer phase propensity of the membrane Pyotr Kisselev1,*, ... correlation between the activation of the CYP1A1- catalyzed epoxidation reaction of 7,8-diols a nd the enhanced nonbilayer phase propensi ty in membranes containing PtdEtn.Effect of lipids on the stereoselectivity ... that in addition to the membrane charge, the nonbilayer phase propensity of the membrane is animportant determinant for an effective reconstitution of CYP1A1- dependent epoxidation activity. The...

Tài liệu Báo cáo khoa học: "Using adaptor grammars to identify synergies in the unsupervised acquisition of linguistic structure" docx

... We initialize the sampler by randomly as-signing each string in the training corpus a randomtree generated by the grammar. Then we randomlyselect a string to resample, and sample a parse of ... grammar, in which the subtrees are speciﬁed in advance, in an adaptor grammar the subtrees, aswell as their probabilities, are learnt from the train-ing data. In order to make parsing and inferencetractable ... terminal in the grammar. If Ais a terminal then GAis the distribution that puts all of its mass on the unit tree (i.e., tree consisting of asingle node) labeled A. If A is a nonterminal thenGAis...

Báo cáo khoa học: Testosterone 1b-hydroxylation by human cytochrome P450 3A4 pdf

... baculovirus-infected insect cells contain-ing 4 pmol of P450 3A4. (Varying amounts of P450 wereused because of differences in rates of the systems, in orderto maintain linearity of product formation vs. ... ay as one of the characteristic assays of P450 3A4, which wassubsequently shown to be the most abundant P450 in human liver and small intestine [ 22] and involved in t heoxidation of approximately ... is of interest to note that almost all of the P450 3A4-catalyzed hydroxylations of testosterone are on the b face.This information is of interest in considerations of the steroselectivity of...

Báo cáo khoa học: "Learning to Win by Reading Manuals in a Monte-Carlo Framework" pot

... baselines – on average winning53.7% of all games within the ﬁrst 100 steps. The dismal performance, on the other hand, of both the random baseline and the game’s own built -in AI(playing against ... yielding a27% absolute improvement and winning over78% of games when playing against the built- in AI of Civilization II.11 Introduction In this paper, we study the task of grounding lin-guistic ... containing randomtext. We generate this text by randomly permut-ing the word locations of the actual game manual,thereby maintaining the document’s overall statisti-cal properties. The second...

Báo cáo khoa học: PCSK9 is phosphorylated by a Golgi casein kinase-like kinase ex vivo and circulates as a phosphoprotein in humans doc

... (a) they occur ex vivo in human liver cell lines and in vivo in human plasma;(b) in the case of the propeptide, phosphorylation isdecreased in the naturally occurring ‘loss -of- function’R46L and ... autocatalyticprocessing of its prodomain in the endoplasmic reticulum and that itsinhibitory prosegment remains associated with it following secretion.Herein, we used a combination of mass spectrometry and ... isphosphorylated in a cell-type speciﬁc mannerWe examined the heterogeneity of the molecular mass of the propeptide of endogenous PCSK9 in the media of HepG2 cells by MS analyses of immunoprecipitateswith...

Báo cáo khoa học: Myocyte enhancer factor 2B is involved in the inducible expression of NOX1⁄ NADPH oxidase, a vascular superoxide-producing enzyme ppt

... arrows.(B) The exon ⁄ intron structure of the NOX1gene and its splicing pathway. Open boxesindicate exons. Numbers in the box denote the size of each exon (bp). The size of the intron is indicated ... affect the mobility of the speciﬁc bands (lane 4, Fig. 4B). These resultssuggest that PGF2aincreases the binding of MEF2to the consensus-binding site located between )139 and )130 of the NOX1 ... )125 in the 5¢-ﬂanking region containing aconsensus binding site for myocyte enhancer factor 2 (MEF2), to whichbinding of MEF2 was augmented by PGF2a. Gene silencing of MEF2B by RNA interference...

Tài liệu Báo cáo khoa học: Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural speciﬁcity variants doc

... FEBSbean and soybean. When binding of arginine in the presence of tRNA was investigated, some changes in the binding architecture were observed [35], in thatN(153:Y), in addition to interacting with ... arginylated in the presence of arginyl-tRNA synthetase fromjack bean (diagonal shading) or soybean (vertical shading), and the level of charging was compared with that in the presence of the E. ... level attained in the pres-ence of L-arginine was compared to that in the presence of 1 mM of the analogue indicated, using [32P]-labelled jack bean transcript tRNA.Inset: Activity of arginyl-tRNA...

Tài liệu Báo cáo khoa học: "Enhanced word decomposition by calibrating the decision threshold of probabilistic models and using a model ensemble" pdf

... into training and test subsets with the ratio of 9:1 we randomly split the data into training, validation and test sets with the ratio of 8:1:1. We then run our experiments and measured contingency ... Hafer and Weiss, 1974). Another way of classifying ap-proaches is based on the learning aspect during the construction of the morphological model. If the data for training the model has the same ... PROMODES and PROMODES-H the f-measure increased by 0.0228 and 0.0353 on the test set. In short, we have shown that by combiningPROMODES and PROMODES-H and ﬁnding the optimal threshold, the ensemble...

Báo cáo khoa học: The natural mutation by deletion of Lys9 in the thrombin A-chain affects the pKa value of catalytic residues, the overall enzyme’s stability and conformational transitions linked to Na+ binding pdf

... altering the geometry and protonation state of the residues involved in catalysis and inhibitor binding, and (b) limiting the allosteric effects triggered by sodium binding.X-ray structures of human ... simulation of DK9thrombin in explicit water solvent supports the role of the A-chain in affecting the conformation and catalyticproperties of the B-chain, particularly in some insertionloops of the ... thrombin show that the A-chain closely follows the contour of the catalyticB-chain, hinging the two interacting six-stranded bar-rel-like domains of the B-chain [17]. A well-structurednetwork of...

Báo cáo khoa học: Enhanced peptide secretion by gene disruption of CYM1, a novel protease in Saccharomyces cerevisiae doc

... intracellularmaturation of CCK-22 was investigated in an in vitroprotease assay using a c rude extract of S. cerevisiae toanalyse the processing of synthetic human CCK-33 toCCK-22 in the presence of different inhibitors. ... the Lys-speciﬁc processing of proCCK, or that the proteaseis attached to the outer membrane of the mitochondriawith the N-terminus exposed to the cytosol. The precursors of GH1 and proBNP contain potentialprocessing ... nature of the protease performing the cleavage of synthetic human CCK-33 to CCK-22 wasanalysed by inclusion of a number o f inhibitors with the extract from B J2168. Only the a ddition of a metal...

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