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Báo cáo khoa học: Mutagenesis of hydrogenase accessory genes of Synechocystis sp PCC 6803 Additional homologues of hypA and hypB are not active in hydrogenase maturation ppt

Báo cáo khoa học: Mutagenesis of hydrogenase accessory genes of Synechocystis sp. PCC 6803 Additional homologues of hypA and hypB are not active in hydrogenase maturation ppt

Báo cáo khoa học: Mutagenesis of hydrogenase accessory genes of Synechocystis sp. PCC 6803 Additional homologues of hypA and hypB are not active in hydrogenase maturation ppt

... Hoffmann et al. Cyanobacterial hydrogenase accessory genes Mutagenesis of hydrogenase accessory genes of Synechocystis sp. PCC 6803 Additional homologues of hypA and hypB are not active in hydrogenase ... metal insertion process.The hydrogenase activity of DhypA1 and DhypB1 could be increased by theaddition of nickel, suggesting that HypA1 and HypB1 are involved in theinsertion of nickel into ... mutation and not to some unpredictableside effects.Function of hypA and hypB in hydrogenase maturation The function of hypA and hypB was investigated in more detail. Because HypA and HypB were...
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Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx

Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx

... mitogen-activated protein kinase kinase, in vitro and in vivo: analysis of active mutants expressed in E.coli and generation of the active form in stress response in seedlings. Plant J 29, 637–647.40 ... MPK3 and MPK6 phosphorylate OXI1 and PTI1-4. (A)Recombinant kinase-inactive GST-lofMPK3 and GST-lofMPK6 weremixed with HIS-OXI1 in kinase buffer and [c-32P]-ATP. (B) Recombi-nant kinase -active ... (kinase-inactive) forms of theMAPKs were produced as GST-lofMPK3 and GST-lofMPK6. However, when lofMPK3 and lofMPK6proteins were tested for phosphorylation with OXI1,no phosphorylation of...
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Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf

Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf

... been found toincrease the amount of complex containing MT1-MMP and furin, and the expression of a catalyticallyinactive dominant negative furin construct affected theprocessing of pro-MT1-MMP. ... thatbinding of MT1-MMP to GRASP55 could be regu-lated via tyrosine phosphorylation.Surprisingly, MT1-MMP and furin ICDs were alsofound to interact with the C-terminal region (region 3) of GRASP55. ... comprising animportant sequence for the interaction with the PDZproteins: Mint-3, AMPA binding protein and gluta-mate receptor interacting protein (GRIP). It wouldtherefore be interesting to...
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Tài liệu Báo cáo khoa học: An ecdysteroid-inducible insulin-like growth factor-like peptide regulates adult development of the silkmoth Bombyx mori docx

Tài liệu Báo cáo khoa học: An ecdysteroid-inducible insulin-like growth factor-like peptide regulates adult development of the silkmoth Bombyx mori docx

... familypeptides in other insects showing such an expressionpattern are good candidates for being the functionalequivalent of BIGFLP. Although bombyxin genes and the majority of known insect ILP genes are ... similar amino acid sequences, they havedistinct domain organizations and physiological func-tions: insulin is a heterodimeric peptide consisting of an A-chain and a B-chain, whereas IGFs are single-chain ... than to those of bombyxin and insulin,which are of the order of 100 pm [23,24]. The veryhigh titer of 8K-BLP may be due to a remarkably highlevel of gene expression in the fat body and to thelarge...
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Tài liệu Báo cáo khoa học: Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural specificity variants doc

Tài liệu Báo cáo khoa học: Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural specificity variants doc

... FEBSbean and soybean. When binding of arginine in thepresence of tRNA was investigated, some changes in the binding architecture were observed [35], in thatN(153:Y), in addition to interacting with ... level attained in the pres-ence of L-arginine was compared to that in the presence of 1 mM of the analogue indicated, using [32P]-labelled jack bean transcript tRNA.Inset: Activity of arginyl-tRNA ... December2008)doi:10.1111/j.1742-4658.2009.06866.xl-Canavanine occurs as a toxic non-protein amino acid in more than 1500leguminous plants. One mechanism of its toxicity is its incorporation intoproteins, replacing l-arginine and giving rise...
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Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc

Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc

... restingcells of a mutant, strain Y-2, of the aniline-assimilatingPseudomonas sp. strain AW-2 [20].ResultsSpectral changes during metabolism of 4-amino-3-hydroxybenzoic acid by crude extracts of strain ... substrate. Insights into the metabolicfate of 4-amino-3-hydroxybenzoic acid in strain 10d are revealed.Materials and methodsBacterial strain and growth conditionsBordetella sp. strain 10d was ... enzyme responsible for thedeamination of a chemically unstable intermediate in the metabolicpathway of 4-amino-3-hydroxybenzoic acid in Bordetella sp. strain 10dChika Orii1, Shinji Takenaka2,...
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Tài liệu Báo cáo khoa học: What makes biochemical networks tick? A graphical tool for the identification of oscillophores ppt

Tài liệu Báo cáo khoa học: What makes biochemical networks tick? A graphical tool for the identification of oscillophores ppt

... Netherlands;3Department of Biochemistry and Molecular Biology, Faculty of Chemistry and CeRQT at BarcelonaScientific Parc, University of Barcelona, Spain In view of the increasing number of reported concentrationoscillations ... species.Reactions involving more than one molecule of a singlespecies are described as a sequence of two independentreactions. Assuming s patial homogeneity and a sin glecompartment, the kinetic equations ... of asubgraph that contains p reactions and p concentrations, asminus the sum of all its combined autoinfluence paths of order p. Note therefore that negativity of a subgraph and positivity of...
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Tài liệu Báo cáo khoa học: N-Methyl-L-amino acid dehydrogenase from Pseudomonas putida A novel member of an unusual NAD(P)-dependent oxidoreductase superfamily ppt

Tài liệu Báo cáo khoa học: N-Methyl-L-amino acid dehydrogenase from Pseudomonas putida A novel member of an unusual NAD(P)-dependent oxidoreductase superfamily ppt

... discriminatingbetween methylamine and ammonia are poorly under-stood. N-Methyl amino acids are structurally similarto opines such as nopaline, octopine, lysopine, and histopine, which are N-substituted ... naturally as components of several depsipeptides, including cyclosporin A [1],vancomycin, destruxins [2], didemnins [3], dolastatin[4], and enniatin [5], as well as a calpain inhibitor fromStreptomyces ... relative to methylamine) were found withethylenediamine, hydroxylamine, isopropylamine, n-butylamine, n-amylamine, n-hexylamine, 1,6-diamino-hexane, and spermidine. Interestingly, the enzyme...
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Tài liệu Báo cáo khoa học:

Tài liệu Báo cáo khoa học: "Mining User Reviews: from Specification to Summarization Xinfan Meng Key Laboratory of Computational Linguistics " doc

... Laboratory of Computational Linguistics(Peking University)Ministry of Education, Chinamxf@pku.edu.cnHoufeng WangKey Laboratory of Computational Linguistics(Peking University)Ministry of Education, ... struc-ture information and unit of measurementinformation are mined from the specifi-cation to improve the accuracy of featureextraction. At summary generation stage,hierarchy information in specifications ... Some features are present in every product specification. But there are cases that some features are not available in allspecifications. For instance, “WiFi” features are only available in a few mobile...
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Tài liệu Báo cáo khoa học: A peptide containing a novel FPGN CD40-binding sequence enhances adenoviral infection of murine and human dendritic cells doc

Tài liệu Báo cáo khoa học: A peptide containing a novel FPGN CD40-binding sequence enhances adenoviral infection of murine and human dendritic cells doc

... CD40-binding peptide that has no detectablehomology to CD40 ligand (data not shown) and thatenhances adenoviral infection of CD40-positive cells of bothhuman and murine origin, including DC and ... amplified using the E. coli ER2738 host strain(New England Biolabs, Inc.) and subjected to two additional rounds of biopanning and amplification. Upon completion of three rounds of biopanning, individual ... DC,monocyte/macrophages and B cells. Cross-linking of CD40,following its interaction with CD40 ligand (CD40L) onactivated helper T lymphocytes, induces activation and maturation of antigen presenting cells [1]. In...
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