Báo cáo khoa học: Transcription termination at the mouse mitochondrial H-strand promoter distal site requires an A/T rich sequence motif and sequence specific DNA binding proteins pptx
Báo cáo khoa học: Transcription termination at the mouse mitochondrial H-strand promoter distal site requires an A/T rich sequence motif and sequence specific DNA binding proteins pptx
... termination at the mouse mitochondrial H-strand promoter distal site requires an A/T rich sequence motif and sequence specific DNA binding proteins Vijayasarathy Camasamudram, Ji-Kang Fang and Narayan ... L and H strand promoters (LSP and HSP), L and H strand transcription start sites (LSS and HSS). The L-strand sequence and position of the putative promoter distal transcription ter-mination element ... understand the nature of D-TERM DNA binding proteins and their possible role in transcription Fig. 1. Location of the putative D-TERMelement for H-strand transcription termination on the mouse...
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Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll fluorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc
... indicatesthat, for all treatments, the FI rise was faster at 25 °Cthan at 15 °C and that the contribution from the O–Jphase decreased at high temperature. Figure 3 repre-sents the amplitudes and ... in the FI curves depends on the relative amplitude and rate constant of the J–Iphase compared to the amplitudes and rate constantsof the two other phases. The above should be gov-erned by the ... uorescence at time t, F0is the initialuorescence, AOJ, AJI and AIPare the amplitudes, and kOJ, kJI and kIPare the rate constants of the OJ, JI and IP steps of the uorescence transient.EAvalues...
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Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt
... mutants, the relationship between the DEC1–BMAL1 interactio n and transcriptional suppressionby DEC1. We also evaluated the E-box -binding activity ofthese mutants. Our results showed that the ... blot analys is with anti-FLAGimmunoglobulin (left-hand panel). The transcriptional activities of FLAG-BMAL1 and FLAG-DEC1 were confirmed by using the luciferase assay(right-hand panel). The values ... for the bHLH domain-,basic region- and Orange domain-deleted DEC1 mutants,three sets of primers (5¢-ATTGATCAGC AGCAGCAGAAAATCATTGCC-3¢ and 5¢-CTTGCTGTCCTCGCTCCGCTTTATTCCC-3 ¢ for DEC1DbHLH and DEC1:4–232DbHLH;...
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Báo cáo khoa học: NF-jB regulates the transcription of protein tyrosine kinase Tec pdf
... Tec-920 and Tec-1800; two putative NF-jB binding sites are indicated, and the knownPU.1 and SP1 binding sites are also shown. (B) Schematic representation showing the potential NF-jB binding sites ... to the Tec promoter and induces Tec transcription. (A) The chromatin of A20 cells was cross-linked,sheared and immunoprecipitated with the indicated antibodies.Input and immunoprecipitated DNAs ... A20, U937 and HEK293T(data not shown). Collectively, our results show thatNF-jB can bind to both human and mouse Tec pro-moters and induce their transcriptional activity, and proteasome and NF-jB...
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Báo cáo khoa học: Novel repressor of the human FMR1 gene ) identification ¨ of p56 human (GCC)n-binding protein as a Kruppel-like transcription factor ZF5 ppt
... the negative control fraction remained in afinal fraction. There were also two bands at 65 kDa and 72 kDa that were detected in both the negative control and final fractions. The p56 and p68 proteins ... [18–20] and within the 5¢-UTR of the human very low densitylipoprotein receptor gene (VLDLR) [21]. Such GCC-elements are transcriptionally active and can interactspecifically with human and mouse ... of the rpL32 promoter and the 5¢-UTR of the humanFMR1 gene. We further identify p56 as the Kru¨ppel-like transcription factor ZF5. We show, for the firsttime, that recombinant mammalian ZF5...
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Báo cáo khoa học: Sequences downstream of the transcription initiation site are important for proper initiation and regulation of mouse ribonucleotide reductase R2 gene transcription ppt
... of the mouse and human R2 DNA sequences around their transcription start sites. Capital letters in bold style indicate nucleotides that areconserved between human and mouse; arrows indicate the ... numbers represent the intensity of each band normalized to the band in lane 1. The lower numbers represent the intensity of the bandsin lanes 5 and 6 normalized to the band in lane 4.1798 I. Kotova ... templates TATAWtUTRmut and TATAmutUTRmut). We found that both TBP (Fig. 2A,compare lanes 4 and 5) and the R2 TATA-box (Fig. 2A,compare lanes 2 and 3) were absolutely required fortranscription...
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Báo cáo khoa học: "Medical treatment for the terminally ill: the ‘risk of unacceptable badness’"
... concur that there isno meaningful chance of reanimation [8].Some reasons why this occurs are as follows:1. Physicians tell surrogates that they can make any decisionthey want as an open-ended ... regarding treatment options because of theirproximate knowledge of what the patient would have wantedbefore they became incompetent [7]. This position is basedon the postulate that any attempt ... convert death into life-in-death[4]. Patients may be stalled in suspended animation; they arenot alive in the sense the we enjoy life but neither are theyable to die as long as nutrition, hydration,...
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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... ion and the addi-tional water oxygen were only 0.03 and 0.04 A˚, respec-tively, out of the equatorial plane defined by the otherthree atoms (Table 3). The angles around the metalion indicated ... Sedimentation equilibrium distributionof ApeSOD. The line reflects the best fit of the data and indicatesthat the apparent molecular mass is 96 265 Da. The deviationbetween empirical data and the ... the superox-ide substrate coordinates to the metal center from the equatorial plane, and the coordination site is the sameas that of the additional water molecule in the Fe-bound ApeSOD octahedral...
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Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt
... PAGE and R2F-antibody as a marker to assistin the purification of the R2F-protein. In the new puri-fication strategy that was developed (see Materials and methods), an increase in the putative ... man-ganese in the transcriptional regulation of the nrdoperon [35] may not be considered in the light of the results obtained in the present study. Rather, the involvement of manganese in the ... together, these obser-vations demonstrate conclusively that the purified pro-tein was R2F and that it contained a manganesemetallocofactor. The decisive step for purification of the manganese...
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Tài liệu Báo cáo khoa học: Nucleolin/C23 mediates the antiapoptotic effect of heat shock protein 70 during oxidative stress pptx
... condensation and DNA fragmentation [1,2], apoptosis can cause irreversibleloss of terminally differentiated cardiac myocytes and, therefore, contributes significantly to the pathogenesisof many ... in the nucleolus, nucleus and cytoplasm of the cell, aswell as at the cell surface [10]. Nucleolin ⁄ C23 regulatesmany aspects of DNA and RNA metabolism, such asRNA polymerase I-mediated transcription, ... together, our results demonstrate an impor-tant relationship among the regulation of nucleo-lin ⁄ C23, the activation of caspase-3 and the inductionof apoptosis under the setting of oxidative...
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