Báo cáo khoa học: Cytotoxic activity of nucleoside diphosphate kinase secreted from Mycobacterium tuberculosis pptx
Báo cáo khoa học: Cytotoxic activity of nucleoside diphosphate kinase secreted from Mycobacterium tuberculosis pptx
... 1814–1818.634 P. Chopra et al. (Eur. J. Biochem. 270) Ó FEBS 2003 Cytotoxic activity of nucleoside diphosphate kinase secreted from Mycobacterium tuberculosis Puneet Chopra1,2, Anubha Singh1,3, Anil ... (H117Q)resulted in loss of both ATP binding and hydrolysis activity (Fig. 3). Thus, H117 is crucial for ATPase activity. Secretion of nucleoside diphosphate kinase byM. tuberculosis M. tuberculosis H37Rv ... 2003 Nucleoside diphosphate kinase of Mycobacterium (Eur. J. Biochem. 270) 631 of M. tuberculosis to promote apoptosis may also beimportant for dissemination of infection.Aknockoutmutant of Ndk...
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Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx
... mechanisms of calpain 7 in vitro:(a) by binding of MIM, MIT domains dissociate from the protease domain to expose the catalytic core; and(b) binding of MIM causes a conformational change of calpain ... the C-terminal half of PacC [16]. A human ortholog of PalA, ALIX (also known as AIP1), functions in thebudding of enveloped RNA viruses from plasmaY. Osako et al. Enhancement of calpain 7 autolysis ... the case of mGFP–calpain 7C290S,suggesting that the degradation was caused by proteo-lytic activity of mGFP–calpain 7 itself. We refer to thisissue later.Effects of mutations of IST1 MIMs...
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Tài liệu Báo cáo khoa học: ATPase activity of magnesium chelatase subunit I is required to maintain subunit D in vivo ppt
... expected size of a DNA fragment amplified from the cDNA was 378 bp, whereas the size of a DNAfragment amplified from genomic Xantha-g DNA was566bp.DNAfragmentsof378bpcouldbeisolatedfromthe wild-type ... encoding a third subunit, analysis of the interaction of the three subunits with the yeast two-hybrid system, and recon-stitution of the enzyme activity by co-expression of recombinantCHL D, CHL H ... contactwith the integrin-binding motif of BchH, simultaneouslytriggering porphyrin metallation. This would also lead to arelease of the blockade of the ATP-binding site of BchI bythe integrin I domain,...
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Tài liệu Báo cáo khoa học: High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine pptx
... themechanism of action of chymotrypsin: the role of the low barrierhydrogen bond. Biochemistry 36, 4576–4584.46. Warshel, A. (1998) Electrostatic origin of the catalytic power of enzymes and the role of ... results from the strong polarization of the C–O bond by the dipoles of the oxyanion hole in conjunction with the influence of aclose nucleophile like the Oc of the catalytic serine. Thesame type of ... general features of therecently determined X-ray structure of human BuChE [7,8].In particular, most of the essential features of the catalyticsite (i.e. a catalytic triad of Ser-His-Glu, an...
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Báo cáo khóa học: Chaperone activity of cytosolic small heat shock proteins from wheat pptx
... of its chaper-one activity has been performed. Wheat TaHsp17.8C-II is 33% identical overall to TaHsp16.9C-I. Only two previ-ous studies have examined the chaperone activity of thisclassofsHsp,andnoplantclassIIsHsphasbeentestedfor ... the ratio of sHsp to substrate that is required tosuppress light scattering can be used as a measure of effectiveness of the chaperone. Using this assay we tested therelative activity of the two ... exhibit ATP-independent molecular chaperone activity. We studied thechaperone activity of dodecameric wheat TaHsp16.9C-I, aclass I cytosolic sHsp from plants and the only eukaryoticsHsp for...
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Báo cáo khoa học: Antimicrobial activity of histones from hemocytes of the Pacific white shrimp ppt
... inhibited growth of the test bacterium M. luteus at a c oncentration of 4.5 lM.Histone H2A from a variety of v ertebrate species has beenshown to poss ess an timicrobial activity, but its activity appears ... the results of a proteomic investigation of hemocyte antimicrobial peptides of the P acific whiteshrimp, L. vannamei, and the r ole of histones as potentiallyimportant components of their immune ... peptides are also derived from larger proteins by proteolysis. For example, bovinelactoferricin is derived from lactoferrin, an iron bin dingprotein, by pepsin cleavage of its N-terminus [9]....
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Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt
... surface of the molecule and is ratherfar away from the trinuclear site. Therefore, theC-terminal sequence of RlL might be more of an evolu-tionary relic than a functional feature of the enzyme.The ... at pH levels ranging from 2 to 8.AcknowledgementsWe are grateful to M. Paloheimo from Roal Oy forproviding us with samples of TaLcc1. The ESRF isthanked for provision of synchrotron facilities. ... Academy of Finland (Project 115085)supported the work at Joensuu, and part of the VTTwork was carried out with financial support from theMarie Curie EU-project ‘Enzymatic tailoring of pro-tein...
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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... Three of the ligands,OD2 of Asp165, NE2 of His79 and NE2 of His169,formed an equatorial plane. The other protein ligand,NE2 of His31, bound to the metal, in the company of a water oxygen, from ... conformationaround the active site of ApeSOD is independent of the presence of a metal cofactor.Figure 3D shows the superimposition of the activesite structures of apo, Mn-bound and Fe-bound Ape-SODs. ... the presence of either of Mn or Fe. The crystal structures of the superoxide dismutase from A. per-nix in the apo, Mn-bound and Fe-bound forms were determined at resolu-tions of 1.56, 1.35...
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Tài liệu Báo cáo khoa học: Structural features of proinsulin C-peptide oligomeric and amyloid states pptx
... widerange of spectroscopic techniques.Suitable methods for investigation of the secondarystructure and morphology of such structures, however,require the presence of large amounts of the oligomericstate, ... distribu-tion of 100 lM proinsulin C-peptide in thepresence of divalent Ca2+and Mg2+ionsunder native conditions (B), and of 100 lMC-peptide in the presence of insulin (C) and of 100 lM ... population of monomerswith a hydrodynamic radius of approximately 16 A˚,which is in agreement with the results of the NMR.The addition of SDS to the C-peptide sample leads toformation of larger...
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Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt
... prior studies of nonhemeFe2+centers [29–31], is assigned to the mixing of the4p orbital with the 3d orbital of the metal cofactor.Two important pieces of information can be gleaned from the pre-edge ... aliquots of 35 lm or 70 lm from a3.7 mm stock solution); xanthine (360 lm) in the presence of 2-ketoglutarate (1 mm); catechol (200 lm); and quercetin(170 lm). The duration of the assay varied from ... The assistance of T. Pavkov(Institute of Chemistry, University of Graz) in theacquisition of CD and DLS data is gratefully acknowl-edged. B. Kuczewski and H. Wiltsche (Institute of Analytical...
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