Báo cáo khoa học: O-MACS, a novel member of the medium-chain acyl-CoA synthetase family, speciﬁcally expressed in the olfactory epithelium in a zone-speciﬁc manner doc

... Y. Oka et al. (Eur. J. Biochem. 270) Ó FEBS 2003 O-MACS, a novel member of the medium-chain acyl-CoA synthetase family, specifically expressed in the olfactory epithelium in a zone-specific manner Yuichiro ... manner by using the differential displaymethod. Among the clones isolated from the rat OE, wecharacterized a novel olfactory protein termed O-MACS, a member of the medium-chain acyl-CoA synthetase ... vomeronasal epithelium; VLACS, very long chain acyl-CoA synthetase. Enzymes: Medium-chain acyl-CoA synthetase (EC 6.2.1.2).Notes: DNA databank of Japan (DDBJ) accession number for the O-MACS sequence...

Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx

... Interestingly, annotation of the DNAsequences ﬂanking the Jannaschia sp. CCS1 HYDJsrevealed an ORF encoding a putative allantoate amido-hydrolase, which is part of the urate catabolic pathway in ... Tempe, AZ, USA4 Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, ChinaOptically pure d-orl-amino acids are used as inter-mediates in several industries. d-amino acids areinvolved ... databases haveprovided an additional source for identifying d-hydan-toinases with high catalytic activity. In this study, anapproach combining genomic database mining andactivity screening...

Tài liệu Báo cáo khoa học: Characterization and functional expression of cDNAs encoding thyrotropin-releasing hormone receptor from Xenopus laevis Identiﬁcation of a novel subtype of thyrotropin-releasing hormone receptor ppt

... (5¢-GATGTCACGCAGAGTGAGCAGGTAG-3¢)/TRHR-7(5¢-GAGACCATACAGAAC-C-3¢); second set, A5 2 (5¢-AGAGTGAGCAGGTAGCGAGAGGAG-3¢)/TRHR-8(5¢-GGGGGTGTAGAGGTTTCTGGAGAC-3¢); thirdset, A5 3 (5¢-CGAGAGGAGCATTAGA-TAGATGCAG-3¢)/TRHR-9 (5¢-GCCGAAATGTTGATGCCCAGATAC-3¢) ... usingpartially overlapping cDNA fragments and the pair of primers TRHR1-2 sense (5¢-ATAATGGATAACGTAACTTTTGCTG-3¢)/TRHR1-4 antisense (5¢-TCTGTTAAATGTACCTAAGTAGGCA-3¢)andTRHR2-2sense (5¢-CAGCAAAATGGAAAATAGTAGC-3¢)/TRHR2-4 ... pars intermediacells of the frogs, indicating that TRH stimulates the phospholipase C pathway in melanotrope cells [10]. In thesecells, TRH induces also an increase of the intracellularcalcium...

Báo cáo khoa học: Identiﬁcation and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot

... domain B Ligand binding domain Fig. 1. Sequence alignment. (A) Alignment of DNA binding domain (C domain) and itsC-terminal extension. (B) Alignment of ligandbinding domain (E domain) (after ... gene assay in in vivo results.Experimental proceduresParasites The NMRI strain of S. mansoni was maintained in snails(Biomphalaria glabrata) and Syrian golden hamsters(Mesocricetus auratus ... [21]. A SmNR1 speciﬁc probe of 497 bp wasproduced by PCR ampliﬁcation with TOPO 2.1-SmNR1 as a template (forward primer: 5¢-ATTTCAGAAGTTGAACAAACACAC-3¢, reverse primer: 5¢-AAGATGGTATTGAAGATGATGGTTGA-3¢),...

Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx

... is a novel splice variant of ANKHD1 and may play a role in cellular apoptosis (antiapoptotic) andcell survival pathway(s).AbbreviationsANK, ankyrin repeat motif; ANKHD1, ankyrin repeat and ... contain a KHdomain. RNA analysis and blast analysis indicatedthat homologs and orthologs of VBARP exist, indica-ting the presence of VBARP in diverse phyla such asplants, yeast, and eukaryotes. ... Oncogenes and Related Genes. ShanghaiCancer Institute, Shanghai, China.8 Smith RK, Carroll PM, Allard JD & Simon MA (2000)MASK, a large ankyrin repeat and KH domain-con-taining protein involved...

Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt

... DiscussionSulfonimides as inhibitors of RNase A We began by determining the ability of three backboneanalogs of RNA to inhibit catalysis by RNase A. These analogs have a simple polyanionic backbonewith neither a ... USAIntroductionUpon catalyzing the cleavage of RNA, RNases operateat the crossroads of transcription and translation.Bovine pancreatic RNase A (EC 3.1.27.5) is the bestcharacterized RNase. A notoriously stable ... further development and use of N-acylsulfonamides and sulfonimides as antagonists of nucleic acid-bindingproteins.DatabaseStructural data for the two RNase A complexes are available in the...

Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf

... is a proteinaseinvolved in the remodelling of extracellular matrix and the cleavage of a number of substrates. MT1-MMP is synthesized as a zymogen that requiresintracellular post-translational ... (GRASP55). Further analysis revealed that GRASP55 associated with the cytoplasmic domain of both proteases and that the LLY573motif in the MT1-MMP intracellular domain was crucial for the interaction ... 3169MT1-MMP and furin can interact with GRASP55.Overexpression of GRASP55 has been found toincrease the amount of complex containing MT1-MMP and furin, and the expression of a catalyticallyinactive...

Tài liệu Báo cáo khoa học: Structural and functional investigations of Ureaplasma parvum UMP kinase – a potential antibacterial drug target ppt

... andpart of the His-tag was observed in the B chain. A fewamino acid residues are found in disallowed regions in the Ramachandran plot; these are A4 , A1 65, A1 67, B3, B4 andF165, all of which are ... Serina L, Blondin C, Krin E, Sismeiro O, Danchin A, Sakamato H, Gilles A- M & Baˆ rzu O (1995) Escherichiacoli UMP-kinase, a member of the aspartokinase family, is a hexamer regulated by guanine ... main-chain and medium side-chain restraints. Modelbuilding was carried out in o [29] and coot [30]. In chain A, residues 1 and 169–176 are missing; in chain B, residues 167–172 are missing; in...

Tài liệu Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera pptx

... 000 A ˚2) as con-tact area. Thus, a large amount of the available surfacearea of the molecule is buried upon pentamerization,increasing the stability of the protein. The hAd2 ⁄ 12 penton base ... protein. The penton contains all necessary components for viral attachment andentry into the host cell. After initial attachment via the head domain of the fiber protein, the penton base interacts ... the initial bind-ing of minifiber. The fluorescence emission of the TMRwas assumed to be independent of the binding of the minifiber to the penton base, because it is attached at the C-terminal...

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a sialic acid speciﬁc lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

... 2000-fold increase in speciﬁcactivity. Analysis of the lectin on SDS/PAGE gave a singleband at apparent molecular mass of 34 kDa. The binding afﬁnity of the lectin in the hemolymph of the freshwater ... Bovine submaxillary mucin,which contains mainly 9-O-acetyl- and 8,9 di-O-acety-N-acetyl neuraminic acid was the most potent inhibitor of the lectin. Sialidase treatment and de-O-acetylation of ... SuriyaDepartment of Zoology, Holy Cross College, Rochnagar, Nagercoil Tamil Nadu, India A naturally occurring hemagglutinin was detected in the serum of the freshwater crab, Paratelphusa jacquemontii(Rathbun)....

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