Báo cáo khoa học: The role of helix 8 and of the cytosolic C-termini in the internalization and signal transduction of B1 and B2 bradykinin receptors potx

Tài liệu Báo cáo khoa học: A role of miR-27 in the regulation of adipogenesis ppt

... in obese mice In order to gain insights into the potential biologicallyrelevant role of miR-27 in the regulation of adipose tis-sue functions in vivo, we examined the expression of miR-27 in ... differentiationwas initiated at 48 h post-transfection by maintaining the cells in culture medium containing 2% horse serum. Cells were ﬁxed on day 4 of differentiation and stained with hematoxylin and eosin. ... together, these results illustratea critical and speciﬁc role of miR-27 in the regulation of adipogenic differentiation.miR-27 prevents the induction of PPARc and C⁄EBPa In order to delineate...

Tài liệu Báo cáo khoa học: A role for the intersubunit disulﬁdes of seminal RNase in the mechanism of its antitumor action docx

... mechanism of antitumouraction of BS-RNase is based on the ability of the proteinto resist the neutralizing action of the cytosolic RNaseinhibitor, a resistance due to the dimeric structure of the enzyme. ... Eﬀects of protein RNase inhibitor and substrate on the quaternary structures of bovine seminal RNase.Biochemistry 35, 388 0– 388 5.6. Leland,P.A.,Schultz,L.W.,Kim,B M.&Raines,R.T.(19 98) Ribonuclease ... hypothesize that the cytotoxicactivity of the latter monomers was due to the presence in these proteins of disulﬁde bonds, with their potentialchemical instability. It is well known that in the...

Tài liệu Báo cáo khoa học: Key role of the loop connecting the two beta strands of mussel defensin in its antimicrobial activity docx

... moleculecomprising Ôresidues in the protruding domain consisting of the type VI b-turn and the ﬁrst part of b-strand 3Õ (i.e. the b-hairpin loop and part of the adjoining b-sheet) and Ôresidues in the loop ... obtained by other groupswho also point to the role of the connecting loop of the b-hairpin of defensins. A combination of mutationalanalysis [35] and structural analysis of the plant defensinRs-AFP1 ... is not possible to infer whether the key role of the b-hairpin loop of MGD1 (loop 3) in antimicrobial activity of MGD1 is of general value in the mechanism of action of defensins. However, our...

Tài liệu Báo cáo khoa học: Electrostatic role of aromatic ring stacking in the pH-sensitive modulation of a chymotrypsin-type serine protease, Achromobacter protease I pdf

... deviates from the original position. In the struc-tural deviation, the solvent ASA of the side-chain of His210increased with the decrease in size of the side-chain atresidue 169 (Table 1 and Fig. ... effect of the size of the residue at position 169 may berelated to the solvent accessibility of the side-chain of His210 (Fig. 3C). When the accessibility of the side-chain of His210 increased, the ... accessibility of His210. The size of the side-chain at residue 169 and the pK2showed a clear linear relationship (Fig. 3B). The pK2increased as the size of the residue at position 169 increased.The...

Báo cáo khoa học: Critical role of the plasma membrane for expression of mammalian mitochondrial side chain cleavage activity in yeast pptx

... hydrolysis, resulting in anincrease in intracellular sterol trafficking. It is unclear, then,whether the corresponding increase in pregnenolone pro-duction results from an increase in free sterol ... pregnenolone, including the effect of the steroid products on the yeast sterol synthesispathway, acetylation of the product, availability of the substrate, electron flow and localization of the differentprotein ... the ATF2 gene. Therefore, both free pregnenoloneproduction and the efficient coupling of the SCC and the 3b-HSD activities in yeast require the use of strains lackingthis acetylating activity....

Báo cáo khoa học: Essential role of the C-terminus in Melanocarpus albomyces laccase for enzyme production, catalytic properties and structure pdf

... loosebinding of azide to the trinuclear copper center. As the hydrogen bond between the C-terminus and His140 isbroken, and the stability of the trinuclear site isdecreased, the binding of azide ... preventing binding of oxygen.We analyzed the azide inhibition and determined the inhibition constants for sodium azide, in order to see the effect of the mutation (L559A) on the bindingproperties ... expressedboth in yeast and in T. reesei, was practically inactive.Also, the kinetic parameters were altered. The change of only one amino acid in the C-terminus of the lac-case, giving the Tr(L559G) and...

Báo cáo khoa học: Dual role of Nbs1 in the ataxia telangiectasia mutateddependent DNA damage response ppt

... signaling [23]. Domains similar to the ATM-binding domain of Nbs1 are also found in ATRIP and Ku80 and are required for the interaction of these proteins with the ATM-related kinase, ATR, and the ... activation of the S-phase checkpoint. The Mre11-bind-ing domain is responsible for binding to Mre11 during formation of the Mre11–Rad50–Nbs1 (MRN) complex. The ATM-binding domainat the COOH-terminus ... COOH-terminus (BRCT) domain in itsNH2-terminal region, as well as an Mre11-bindingdomain and an ATM-binding domain in its COOH-terminal region (Fig. 1). FHA and BRCT domains areoften present in...

Báo cáo khoa học: Functional role of the linker region in puriﬁed human P-glycoprotein pot

... release restores the inward-facing state. The linker region of P-gp can be assumedto connect the C-terminal helix of subunit A (shown in red in Fig. 7) with the N-terminal elbow helix of sub-unit ... containingP-gp, the results could be affected by other proteins. In fact, it was reported that the linker region of P-gpinteracts with other proteins such as RING ﬁngerprotein 2 (RNF2) [25] and ... sub-unit B (shown in purple in Fig. 7) in the MsbA dimer. In the inward-facing state, there appears to be lessinteraction between the linker region and each subunitbecause both ends of these two helices...

Báo cáo khoa học: Functional role of Bb-chain N-terminal fragment in the ﬁbrin polymerization process pdf

... sequences in the N-terminal region of the fibrin Bb-chain, have beeninvestigated. Peptides Bb12–26 and Bb26–46, but not Bb40–46, Bb19–26, and Bb26–36, proved to be specific inhibitors of fibrin polymerization. ... activity of synthetic peptides mimick-ing the various fragments of the N-terminal region of the fibrin Bb-chain was investigated. Two peptides,Bb12–26 and Bb26–46, but not Bb19–26, Bb26–36, orBb40–46, ... in the fibrinbC-domain [7]. The ‘B’–‘b’ pairing leads to structuralrearrangements in the D-domain and the strengthening of protofibril lateral contacts [9,10].Many indirect data show that other...

Báo cáo khoa học: Biological role of bacterial inclusion bodies: a model for amyloid aggregation potx

... in the target recombinant protein[22]. The distribution of side chains in the sequence,such as occurs in protein folding, plays a pivotal role in determining the conformational properties of ... problems in the development of the biotechnology industry. Over the last decade, the ﬁnedissection of the quality control system in bacteria and the recognition of the amyloid-like architecture of inclusion ... powerfulbiocatalysts (the embedded proteins acting as immobi-lized enzymes) [12,13]. On the other hand, the ﬁne and timely analysis of the amyloid architecture of IBproteins [14,15] has led to the use of these...

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