Báo cáo khoa học: 3 Cdt1 and geminin are down-regulated upon cell cycle exit and are over-expressed in cancer-derived cell lines potx

... recombinant full-length Cdt1 and geminin (HisT7 -geminin and His -geminin, amount of recombinant protein run in each lane in ng is indicated) and immunoblotted with anti -Cdt1 and anti -geminin Ig. ... and geminin mRNAs are down-regulated in G0During the cell cycle, Cdt1 and geminin mRNA levels are mostly stable and protein levels are primary controlled byFig. 3. Expr ession of Cdt1 and geminin ... protein and RNA level, and geminin p rotein accumulates with a 3 6 hdelay over Cdt1, following serum re-addition. Similarly,mouse NIH3T3 cells down-regulate Cdt1 and geminin mRNA and p rotein...

Tài liệu Báo cáo khoa học: Specific targeting of a DNA-alkylating reagent to mitochondria Synthesis and characterization of [4-((11aS)-7-methoxy-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepin-5-on-8-oxy)butyl]-triphenylphosphonium iodide doc

... triphenyl-phosphonium.(Received 27 February 20 03, revised 15 April 20 03, accepted 12 May 20 03) Eur. J. Biochem. 270, 2827–2 836 (20 03) Ó FEBS 20 03 doi:10.1046/j.1 432 -1 033 .20 03. 036 60.x(NARP), by targeting a restriction enzyme ... 36 . Grossman, L.I., Watson, R. & Vinograd, J. (19 73) The presence ofribonucleotides in mature closed-circular mitochondrial DNA.Proc.NatlAcad.Sci.USA70, 33 39 33 43. 37 . Chappell, ... ofmolecules into mitochondria [ 23, 30 33 ]. Therefore attach-ing the DNA alkylating reagent DC-81 to TPP shouldgive rise to a high local concentration in the vicinity ofmtDNA with the resultant binding...

Tài liệu Báo cáo khoa học: Degradation of tropoelastin by matrix metalloproteinases – cleavage site speciﬁcities and release of matrikines pptx

... 150 mm; Dionex) and elution using a linear binarygradient: 5–65% ACN in 0.1% formic acid in 35 min,maintenance at 65% ACN for 10 min, and 65–5% ACN in 10 min.The typical operating conditions ... identiﬁedcleavage sites, and Ala was found N-terminal to 21 (33 %). Similar results were obtained for MMP-12,where Gly was found N-terminal of 30 (33 %) and Ala N-terminal of 36 (39 %) of the 92 identiﬁedcleavage ... containing the matrikines VPGVG (34 1 34 5),VGVPG (34 4 34 8), and GARPG (38 4 38 8) werereleased by MMP-12 exclusively (Tables 3 and 4). Thesmallest peptides found with bioactive sequencesTable 3. ...

Tài liệu Báo cáo khoa học: parDtoxin–antitoxin system of plasmid R1 – basic contributions, biotechnological applications and relationships with closely-related toxin–antitoxin systems ppt

... point (+1) and the extended )10 and )35 are indicated (blue letters). The ribosome-binding site (RBS) and translation initiation codon(Met) of kis are underlined and shown in red. The N-terminal ... 1 43 (Pt 11), 34 03 34 16.8 Hayes F (20 03) Toxins-antitoxins: plasmid mainte-nance, programmed cell death, and cell cycle arrest.Science 30 1, 1496–1499.9 Yarmolinsky MB (1995) Programmed cell ... FEBSmonomers are involved in RNA binding, and disrup-tion of the orientation of the amino terminal hairpinby the C-terminal tail of the antitoxin inactivates thetoxicity of the protein.The crystal...

Tài liệu Báo cáo khoa học: Novel aspects of heat shock factors: DNA recognition, chromatin modulation and gene expression ppt

... constitutively in cells and binds to and regulates the expression of genes without anyapparent stress intervention [1 ,3] .HSF2 and HSF4 may not be directly involved in thestress response, but rather in cell ... DNA-binding domain (DBD)at the amino-terminus and the oligomerization domain in the central region of the protein [1,4]. The HSFDBD belongs to the ‘winged’ helix-turn-helix family ofDNA-binding ... DBD–DBD interaction is important in theHSF–HSF and HSF–HSE interactions. In HSF DBD–HSE co-crystals, the protein–protein interface consistsof the helix 2 amino-terminus, the turn and the wing[15]....

Tài liệu Báo cáo khoa học: The miRNA-192 ⁄194 cluster regulates the Period gene family and the circadian clock doc

... of colorectal cell lines and NIH3T3 cells overexpressing miR-192 ⁄ 194. NIH3T3) is the control cell line and NIH3T3+ indicates the cells overexpressing miR-192 ⁄ 194.(C) Graph showing the periodicity ... cluster in NIH3T3 cells to identify its effect on the circadian cycle. In NIH3T3 cells, miR-192 and miR-194 are almost undetectable (Fig. 5A). As expected, the intro-duction of miR-Vec-192 ⁄ 194 in ... miRNAs are single-stranded, nonprotein-codingRNA molecules, approximately 19–25 nucleotides in length. By binding to the complementary sites in the 3 UTRs of their target genes, they can induce...

Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding speciﬁcity and target candidates doc

... class of RNA-bindingproteins. RNA 3, 1421–1 433 . 35 Cheong CG & Hall TM (2006) Engineering RNAsequence specificity of Pumilio repeats. Proc Natl AcadSci USA 1 03, 136 35– 136 39. 36 Murata Y & ... thebinding of RNA-binding proteins to 3 UTR tran-scripts has been poorly described in plants.PUF proteins are a large family of RNA-bindingproteins found in all eukaryotes. These proteins ... used are given in Tables S1 and S2.Single amino acid change in APUM-2 and APUM-7 proteinsThe amino acid substitution to generate pYESTrp3APUM-2 ⁄ N fi H and pYESTrp3APUM-7 ⁄ H fi N proteins wasobtained...

Tài liệu Báo cáo khoa học: Mouse recombinant protein C variants with enhanced membrane afﬁnity and hyper-anticoagulant activity in mouse plasma pptx

... containing over 20 grouped PS molecules).We have shown that doubling the PS concentration in the membrane results in an increase in the number ofbinding sites (compare Fig. 3D and 3E) and also in ... ⁄ Q32E ⁄ N33D (QNED);mutant III, P10Q ⁄ S12N ⁄ D23S ⁄ Q32E⁄ N33D (QNSED); mu-tant IV, P10Q ⁄ S12N ⁄ D23S (QNS); mutant V, Q32E ⁄ N33D(ED); mutant VI, D23S (S); and mutant VII, D23S ⁄ Q32E ... majority are buried and are integral to maintain the membrane-binding confor-mation [9, 13] . A few of the Gla-bound Ca2+ are acces-sible to solvent and may play a role in membranebinding. A...

Tài liệu Báo cáo khoa học: The cellulosomes from Clostridium cellulolyticum Identiﬁcation of new components and synergies between complexes ppt

... Severalhemicellulases were identiﬁed, including three xylanases in F1, and severalcellulases belonging to glycoside hydrolase families 9 and 5 and, a cysteinprotease inhibitor were identiﬁed in F5 and ... cluster,beginning with the gene encoding the scaffolding pro-tein named cellulosome-integrating protein C (CipC),followed by genes that code for the major cellulosomalcellulase Cel48F and nine other ... interaction with enzyme-borne dockerin modules.The cellulosomes produced by C. cellulolyticumgrown on cellulose contain  30 dockerin-containingproteins [14]. The majority of these proteins...

Tài liệu Báo cáo khoa học: Comparative studies of endonuclease I from cold-adapted Vibrio salmonicida and mesophilic Vibrio cholerae docx

... 64.0 33 .3 ) 30 .7 ) 3. 7 ) 40.6 ) 36 .8m 67.8 73. 9 6.125 p 65.8 33 .2 ) 32 .6 ) 2 .3 ) 40.6 ) 38 .2m 68.1 73. 8 5.7 30 p 65.5 33 .2 ) 32 .3 ) 1.9 ) 40.6 ) 38 .7m 67.4 73. 7 6.4 37 p 66.1 33 .1 ) 32 .9 ... N-terminus (Thr and Met) are encoded by theexpression vector.An analysis of the amino-acid composition showsthat VsEndA contains 13 more lysines and two fewerarginines than Vc EndA, resulting in ... Microb Technol 19, 33 2 33 8. 32 Bradford MM (1976) A rapid and sensitive method forthe quantitation of microgram quantities of protein uti-lizing the principle of protein-dye binding. Anal Biochem72,...

Xem thêm