... NY. 32 Nijhout HF, Smith WA, Schachar I, Subramanian S,Tobler A & Grunert LW (2007) The control of growth and differentiation of the wing imaginal disks of Mand-uca sexta. Dev Biol 30 2, ... to affinity chromatogra-phy. Aliquots of the input and the eluate of the chromatographywere resolved by Tricine ⁄ SDS ⁄ PAGE and then analyzed by Coo-massie Brilliant Blue staining (left) and ... days of cultivation, the number of the disk cells was approxi-mately 30 % larger than in the control (Fig. 6M). Con-sidering the BrdU incorporation in specific areas of the disks (Fig. 6E), the...