... functioning as nitroreductase and ferricreductase is capable of catalyzing the Fenton reaction.FEBS J 274, 1318–1327.14 Kitamura M, Kojima S, Ogasawara K, Nakaya T,Sagara T, Niki K, Miura K, Akutsu ... argon, andNADH or NADPH was used as the reductant. The observed increase in absorption around 340 nm iscaused by the addition of NADH or NADPH. Based on the amino acid comparison (Fig. 1) and ... a gradient of 0–20% acetonitrile in0.1% trifluoroacetic acid at a flow rate of 0.8 mLÆmin)1.Crystallization, data collection and processing The details of crystallization and data collection...