Báo cáo khoa học: The fabp4 gene of zebraﬁsh (Danio rerio) ) genomic homology with the mammalian FABP4 and divergence from the zebraﬁsh fabp3 in developmental expression pot

... role in the specificity and affinity of ligand binding by mammalian FABP4 [23]. The phylogenetic relationship of zebrafish fabp4 and the H6-FABP gene from Antarctic fishes was deter-mined using amino ... Alignment of the cDNA sequence with the coding sequence of zebrafish fabp4 showedone nucleotide difference in the coding region of exon2, resulting in an alteration of the ninth codon of thisexon from ... identity of zebrafish FABP4 was to mammalian FABP4 (51–53 %), FABP3 (54–56 %) and FABP7 (54–55 %). In contrast to the divergence in amino acidsequence between fish FABP4s (83–88 %) and mamma-lian FABP4s...

Tài liệu Báo cáo khoa học: 3T3-L1 adipocyte apoptosis induced by thiazolidinediones is peroxisome proliferator-activated receptor-c-dependent and mediated by the caspase-3-dependent apoptotic pathway doc

... 30821065 and 30870559 from the China National Nature SciencesFoundation, 2006CB910703 from the Ministry of Sciences and Technology of China, and 07dz05907 from the Committee of Sciences and Technology ... 1 lgÆmL )1 insulin, 1 lm dexamethasone, and 0.5 mm 1-methyl-3-isobutylxanthine. The medium wasreplaced with medium containing 1 lgÆmL )1 insulin after72 h, and then changed every other day.Retroviral ... During leptin-induced adipocyte apop-tosis, the expression of PPARc is increased by 80%[10]. Furthermore, the activation of PPARc stimulatesapoptosis in a variety of cell types, including...

Tài liệu Báo cáo khoa học: Solution NMR structure of ﬁve representative glycosylated polyene macrolide antibiotics with a sterol-dependent antifungal activity doc

... rimocidin(C), candidin (D) vacidin A (E). Right: Views of the NMR ensembles, seen along the longaxis of the polyene with the D-mycosaminefront (indicated with an arrow). The bonds of the hydroxyl ... supporting informa-tion) from the X-PLORlibraries and topology ﬁles of different parts of other molecules taken from the ProteinData Bank [49]. For each molecule, the atoms involved in of the ... macrolides asource of lead structures for the engineering of futuremolecules with improved medicinal purposes. In parti-cular, the gene clusters involved in the biosynthesis of pimaricin in S. natalensis...

Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt

... ribonucle-otide binding and radical transfer from the tyrosyl rad-ical (Y12 2) of the R2 subunit to the cysteine (C43 9) found in the active site of the R1 subunit; (b) the abstraction of two protons and water ... of man-ganese in the transcriptional regulation of the nrdoperon [35] may not be considered in the light of the results obtained in the present study. Rather, the involvement of manganese in ... this RNR and that of other class 1RNRs or, instead, is a result of the interaction of the R2F with the inactivated (oxidized) form of the R1E.We consider the ﬁrst option unlikely. Under these...

Báo cáo khoa học: Spatio-temporal distribution of fatty acid-binding protein 6 (fabp6) gene transcripts in the developing and adult zebraﬁsh (Danio rerio) pptx

... the intestine (A), liver (B), ovary (C) and the adrenal homo-log of the fish kidney (D). Panel 2 shows the locations of the distalregion of the intestine (A), liver (B), ovary (C) and the adrenal ... distribution and binding activities of FABPs and regulation of FABP genes, includingFABP gene knock-out experiments [8], our under-standing of the physiological function(s) of these pro-teins remains ... and adult zebrafish (Danio rerio). Like most members of the vertebrate FABP multigene family, the zebrafish fabp6 gene containsfour exons separated by three introns. The coding region of the gene...

Tài liệu Báo cáo khoa học: Glycomics-based analysis of chicken red blood cells provides insight into the selectivity of the viral agglutination assay docx

... of cRBCs within the region of interest is shown in Fig. 3 and the list of important chemical shifts, along with a schematic of protons and probable assignments, is shown in Table S1. Within the ... hemagglutinins as examples. Finally, recent understanding of the role of various glycan structures in high afﬁnity binding to in uenzahemagglutinins provides context to our ﬁndings. These results ... difference in the ability of SC18 and Alb58 HA to agglutinate cRBC isexplained by minimal presence of sialylated glycans with poly-lactosamine repeats in the cRBCs. Alb58 on the other hand binds to...

Tài liệu Báo cáo khoa học: Minor capsid proteins of mouse polyomavirus are inducers of apoptosis when produced individually but are only moderate contributors to cell death during the late phase of viral infection ppt

... properties of the minor structural proteins (VP2 and VP 3) of the MPyVwere studied in the absence of other virus componentsas well as during the late phase of virus infection. The role of the minor ... as in the release of virus progeny from infected cells. In the late phase of SV40 infection, production of a late protein, VP4 (125amino acids from the C-terminus of VP 3), which trig-gers the ... suggests that the minor capsid pro-teins are not the sole or even the main inducers of apoptosis in the infection process and are dispensablefor cell death. During infection, most of the minorcapsid...

Tài liệu Báo cáo khoa học: An autoinhibitory effect of the homothorax domain of Meis2 ppt

... binding, and indirectly via inter-action with other transcription factors [8,9]. However,even when HD proteins bind their cognate DNA-bind-ing site, they generally bind to other DNA-bindingcofactors ... containing either a consensusMeis2-binding and Pbx1-binding site or the Pbx–Hox-bind-ing site from the Hox B1 ARE were phosphorylated with polynucleotide kinase (NE Biolabs, Ipswich, MA, USA) and ligated ... mouse and human, the 17 codons removed in human Meis3.2 are presentat the 5¢-end of exon 6 of all three genes. In contrast, in the Prep1 gene, the equivalents of exons 5 and 6 in the Meis genes...

Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc

... probes16-doxylstearic acid in the interior of the micelle (A) and Mn2+ in the aqueous buffer (B) selectively attenuate distinct regions of VpU-cyt, reﬂecting the topology and the dynamics of the micelle-associ-ated ... into the micelle and are largely shielded from water(green). In contrast, central residues of the interhelicallinker extend away from the detergent–water interface(red). Both ends of the interhelical ... a-helix 2 and may even be of the 310kind. Most residues in the interhelical linker(R49–E6 3) and the C-terminal region of VpUcyt from G71 to L81 exhibited more intense daN(i,i + 1) thandNN(i,i...

Tài liệu Báo cáo khoa học: Substrate speciﬁcity of the pseudouridine synthase RluD in Escherichia coli doc

... of one U by C has no effect on the conversion of others (i.e. formation of pseudouridines at positions 1911, 1915, and 1917 are independent of eachother); (c) A1916 is the only position in the ... revealed: (a) RluD isomerizes uridine in vivo only at posi-tions 1911, 1915, and 1917, regardless of the presence of uridine at otherpositions in the loop of helix 69 of 23S rRNA variants; (b) substitution ... rRNA in the 50S subunits and in the 70S ribosomes. 23S rRNA from a strainlacking RluD did not have pseudouridines in H69 asFig. 1. A scheme of E. coli 23S rRNA stem-loop 69 (H6 9). Numbers of the...

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