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Báo cáo khoa học: A new approach for distinguishing cathepsin E and D activity in antigen-processing organelles pdf

Báo cáo khoa học: A new approach for distinguishing cathepsin E and D activity in antigen-processing organelles pdf

Báo cáo khoa học: A new approach for distinguishing cathepsin E and D activity in antigen-processing organelles pdf

... similar proteinases, CatE and CatD, and can therefore be used to investigate the involvementof these enzymes in antigen processing and presentation.Experimental proceduresEnzymes and chemicalsCatD ... antibodies were preincubatedwith different concentrations of CatE orCatD, before standard ELISA. ELISA wasperformed as described in Experimental pro-cedures. The increasing concentration ofCatE ... three cell types tested [30]. In contrast, CatE activity was mainly detected in endo-somal fractions, as indicated in Table 2 and Fig. 5. A low level of CatD activity was determined in endosomal...
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Báo cáo khoa học: A single intersubunit salt bridge affects oligomerization and catalytic activity in a bacterial quinone reductase pptx

Báo cáo khoa học: A single intersubunit salt bridge affects oligomerization and catalytic activity in a bacterial quinone reductase pptx

... visible absorbance spectra were recorded before and after denaturation of the enzyme with a photometer(model specord 205) from Analytik Jena AS (Jena, Ger-many). All measurements were performed in ... chromatographywere verified by native PAGE, in accordance with thestandard procedures for SDS-PAGE, using 12.5% separat-ing gels and 5% stacking gels. The gels and the runningbuffer, respectively lacked ... gen-erated, expressed, purified, crystallized and biochemically characterized.The K109L and D1 37L variants form dimers instead of tetramers, whereasthe K109L ⁄ D1 37L and K10 9D D1 37K variants appear...
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Báo cáo khoa học:

Báo cáo khoa học: "A new Approach to Improving Multilingual Summarization using a Genetic Algorithm" pptx

... Overlap-based edge matching between title and sentence graphsTITLE E J Jaccard-based edge matching between title and sentence graphs D COV E O Overlap-based edge matching between sentence and a document ... nodes having a degree higherthan a predefined thresholdDEG Average degree for all sentence nodes:i∈{words(S)}DegiNGRASE Frequent sentences from bushy paths are selected. Each sentence in ... DEG Graph-based extensions of LUHN, KEY and COV measures respectively.KEY DEG Node degree is used instead of a word frequency: words are consideredCOV DEG significant if they are represented...
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Báo cáo khoa học:

Báo cáo khoa học: "A New Approach to the Mechanical Syntactic Analysis of Russian" ppt

... 1. An Identification Tag (t) is appended to the occurrence to indicate the page, sentence, and serial number. Its characters are counted and examined for indications anent its physical make-up. ... therein are capitalized when first encountered in the text. (It is planned to expand this information to include diacritical material designed to aid in the semantic analysis of the sentence.) ... and to obtain the same results,—such a transla- tion may be regarded as a practical one. Perhaps the translation is not couched in elegant terms; here and there several alternative meanings...
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Báo cáo khoa học: A new paradigm for oxygen binding involving two types of ab contacts docx

Báo cáo khoa học: A new paradigm for oxygen binding involving two types of ab contacts docx

... sites in the b4tetramer areindeed different from the corresponding a1 b1 contact sites in the HbA tetramer.Anyway, we have revealed that over the wide range ofpH 5–10, the separated a and ... equations derived therefrom were tested for their fit to the experimental data with the aid of a computer.As a result, the pH-dependence curves for the autoxidationrate of the separated a and ... function was notclear until quite recently. However, detailed pH-dependencestudies of the autoxidation rate of HbO2have revealed thatthe a1 b1anda2b2 interfaces are used for controlling thestability...
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Báo cáo khoa học:

Báo cáo khoa học: "A Clustering Approach for the Nearly Unsupervised Recognition of Nonliteral Language" pdf

... multiple seedset learners, a voting schema, and addi-tional features like SuperTags and extra-sentential context. Detailed experimentson hand-annotated data show that our en-hanced algorithm ... word found in WordNet(literal feedback set) and the DoKMIE (nonliteralfeedback set). The feedback sets also contain ex-ample sentences provided in the target-word en-tries of these datasets. ... literal and nonliteral precision; similarly for av-erage recall. For overall performance, we take thef-score of average precision and average recall.We calculated two baselines for each word....
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Báo cáo khóa học: A new UV-B absorbing mycosporine with photo protective activity from the lichenized ascomycete Collema cristatum docx

Báo cáo khóa học: A new UV-B absorbing mycosporine with photo protective activity from the lichenized ascomycete Collema cristatum docx

... such DNA lesions depends on the capacity ofthe cell to repair the damage before it can be incorporatedpermanently into the genome. Typically, DNA damage isrepaired at a relative high rate in ... formation, HaCaTkeratinocytes were irradiated through a quartz plate onwhich the compound was spread, cells were harvestedimmediately following irradiation and analysed for pyri-midine dimer formation. ... pyrimidine dimer formation in cultured human keratinocytes, and prevention of UV-Binduced erythema. It was found that the pure isolatedcompound prevented UV-B induced cell destruction in a dose-dependent...
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Báo cáo khoa học:

Báo cáo khoa học: "A Discriminative Model for Joint Morphological Disambiguation and Dependency Parsing" ppt

... CASE3,acc and CASE6,accare bolded, indicatingthat w3 and w6are linked and both have the accusative case. The ternary factor CASE-LINK, that connects to thesethree variable, therefore ... as a graphical model. The variables, all boolean, are represented by circles and are bolded if their correct values are true. Factors are represented by rectangles and are bolded if they fire. For ... ar-ticle, and later classic Greek, which has a definitearticle; Hungarian has both a definite and an indefi-nite article. In both languages (Tables 5 and 6), noun and adjective gender, number, and case...
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Tài liệu Báo cáo khoa học:

Tài liệu Báo cáo khoa học: "A New Dataset and Method for Automatically Grading ESOL Texts" pdf

... the language models we developed toestimate the error-rate. In the experiments reportedhereafter, we use the ukWaC+CLC LM to calculatethe error-rate. In order to assess the independent as ... shallow, and the approach doesn’t make effi-cient use of the training data as a separate classifieris trained for each grade point.Recently, Chen et al. (2010) has proposed an un-supervised approach ... the ranks.Table 1 presents the Pearson’s and Spearman’scorrelation between the CLC scores and the AA sys-tem predicted values, when incrementally addingto the model the feature types described...
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Báo cáo khoa học: A new molecular tool for transgenic diatoms Control of mRNA and protein biosynthesis by an inducible promoter–terminator cassette docx

Báo cáo khoa học: A new molecular tool for transgenic diatoms Control of mRNA and protein biosynthesis by an inducible promoter–terminator cassette docx

... dimerization domain (aa 321–447), heme domain (aa 519–592), FADdomain (aa 623–728) and NADH domain (residues 744–858) [27]. A unique 17-amino-acid insertion in the molybdopterin-binding domain iden-tified ... (5¢-CATNCCCATNARRTTCCA-3¢) were designed based on the alignment of thededuced amino-acid sequences of nine NR genes from algae and higher plants. The cDNA was used as template for 35cycles of PCR amplification (15 ... including the5¢-UTR and 3¢-UTR. To determine the 5¢ sequence of theCfNR mRNA, the cDNA was C-tailed as described in [35] and used in a 5 ¢-RACE PCR using antisense primer5¢-CAGCTAAACCCAATAGTCTG-3¢...
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