Báo cáo khoa học: Progressive decrease of phosphocreatine, creatine and creatine kinase in skeletal muscle upon transformation to sarcoma pot

... 2008 FEBS 3247 Progressive decrease of phosphocreatine, creatine and creatine kinase in skeletal muscle upon transformation to sarcoma Subrata Patra1, Soumen Bera1, Soumya SinhaRoy1,*, Sarani ... unable to retain their intra-cellular creatine pool in the form of phosphocreatine.The simultaneous decrease in phosphocreatine, crea-tine and CK in sarcoma tissue suggests that creatine availability ... using such a procedure, and also by injecting existing sarcoma 180 cells that hadbeen previously maintained in the peritoneal cavity of mice. Analyses of phosphocreatine, creatine and CKand...

Tài liệu Báo cáo khoa học: Differential effects of Mxi1-SRa and Mxi1-SRb in Myc antagonism ppt

... predominanttranscript in the adult intestine and in the developingembryo, whereas Mxi1-SRb transcripts predominate in the adult liver and kidney [12]. In our initial description of Mxi1-SRa and ... many of the biological properties attributedoriginally to Mxi1 and outlined above (we haverenamed the original Mxi1 isoform Mxi1-SRb) [12].Speciﬁcally, Mxi1-SRa can also bind to Max and to Sin3, ... Mxi1-SRb in the REF assay In our earlier report describing Mxi1-SRa, this isoformappeared functionally homologous to Mxi1-SRb in thatboth could bind to Max and Sin3 and repress bothbasal and Myc-activated...

Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx

... media To examine changes in iron metabolism parameters weexamined expression of the transferrin receptor, ferritin and storage iron in HepG2 cells grown in media sup-plemented with 100 lm of ferric ... Quantification of DNA contentby flow cytometry revealed that the inhibition of cellgrowth was due to a decrease in the percentage of cells in the S and G2 ⁄ M phases of the cell cycle (Fig. 3B). In a total ... in iron-rich mediashowed a further increase in ROS production as deter-mined by an increase in DCFH mean fluorescenceintensity (Fig. 2B, thick line). In addition, determin-ation of acrolein...

Tài liệu Báo cáo khoa học: Inhibitory properties of cystatin F and its localization in U937 promonocyte cells ppt

... to restore monomers and inhibi-tory activity under nondenaturing conditions. Loss of inhibitory potential of dimerized cystatin F can beexplained by blocking of the N-terminal part, disablingprotease ... processregulating its inhibitory properties.Screening of proteases for their inhibition showedthat cystatin F is different from other cystatins, both in terms of speciﬁcity and strength of binding to ... cystatin C is the mostdiscriminating residue for binding to cathepsins B, H,L, S [24]. No L9K mutants of cystatin C have beenprepared yet to study the effect of incorporating theCystatin F in...

Báo cáo khoa học: Covalent activation of heart AMP-activated protein kinase in response to physiological concentrations of long-chain fatty acids docx

... apoptosis, insulin receptor substrate 1, mammaliantarget of rapamycin kinase (mTOR), mitogen-activatedprotein kinase kinase 3, c-Jun N-terminal kinase, translationelongation factor eEF2 and ... phosphatidylinositol 3 -kinase inhibitorwortmannin, no other details of this insulin process areknown. A novel and physiologically interesting observation of the present study was that palmitate totally ... blockedinactivation of the AMPK by insulin (Fig. 7), suggesting adominance of the fatty acid-driven pathway for activation of AMPK over at least some aspects of insulin signalling.This dominance of...

Báo cáo khoa học: Weak oligomerization of low-molecular-weight protein tyrosine phosphatase is conserved from mammals to bacteria pot

... families of proteintyrosine phosphatases [1]. In eukaryotic cells, lmwPTPsparticipate in platelet-derived growth factor (PDGF)-induced mitogenesis [2] and insulin-mediated mitoticsignaling [3]. ... missing in prokaryotic lmwPTP, whereas the role of its indoleside-chain in the stabilization of the dimer seems to betaken on by the phenyl group of F40 of YwlE.Residues N44 in YwlE and R53 in ... a regulatory mechanism leading to the generation of signaling pulses. The presence of a phenylalanine residue in the dimeriza-tion site of YwlE, replacing a tyrosine residue conserved in all...

Báo cáo khoa học: Acute activation of Erk1/Erk2 and protein kinase B/akt proceed by independent pathways in multiple cell types ppt

... phosphatidylinositol; PtdIns3K, phosphatidylinositol 3 -kinase; PKB, protein kinase B ⁄ akt; PKC, protein kinase C.4372 FEBS Journal 272 (2005) 4372–4384 ª 2005 FEBSlead to activation of downstream kinases includingprotein ... Woodgett J& Dedhar S (1998) Phosphoinositide-3-OH kinase- dependent regulation of glycogen synthase kinase 3 and protein kinase B ⁄ AKT by the integrin-linked kinase. Proc Natl Acad Sci USA 95, ... Phosphoinositide 3 -kinase- dependent regulation of interleukin-3-induced proliferation: involvement of mitogen-activated protein kinases, SHP2 and Gab2.J Biol Chem 276, 24274–24283.PtdIns3K activity...

Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf

... 4625response to glucose, suggesting the importance of PKA in protein trafﬁcking [47,48]. In this study, we usedPKA knockout mutants to determine the role of PKA in mitochondrial targeting of CYP2E1. In ... shown).Role of PKA in the mitochondrial targeting of CYP2E1The role of PKA in mitochondrial targeting of CYP2E1 was investigated using two approaches. Theﬁrst approach involved measuring the level of ... University of Pennsylvania. Journal compilation ª 2007 FEBSMitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomycescerevisiae)role of protein kinase...

Tài liệu Báo cáo khoa học: Down-regulation of reduced folate carrier may result in folate malabsorption across intestinal brush border membrane during experimental alcoholism docx

... counterstaining.Haematoxylin–eosin stainingHaematoxylin–eosin staining was carried out followingthe routine histological method described by Kayser and Bubenzer [38]. The haematoxylin–eosin staining techniqueemploys ... was to delineate the regulatorymechanisms of folate transport in intestinal absorptive epithelia in order to obtain insights into folate malabsorption in a rat model of alcoholism. Therats were ... primers corresponding to a sequence in theopen reading frame of rat RFC and b-actin (as aninternal control); products of 489 and 588 bp for RFC and b-actin, respectively, were obtained on electropho-resis...

Tài liệu Báo cáo khoa học: Identiﬁcation of a novel matrix protein contained in a protein aggregate associated with collagen in ﬁsh otoliths pdf

... determine the calcium-binding domain of the protein. (A) Schematic drawing of the recombinant proteins. SixGST-fused recombinant proteins containing the three distinctivedomains of tandem repeat 1 ... to the beads. By contrast, incubation with otolith extractresulted in binding of a complex of the HMW aggregate containingOMM-64 and otolin-1 to the beads.H. Tohse et al. A novel protein in ... speciﬁcally in otolith matrix-producingcells and deposited onto the otolith. Moreover, the HMW aggregate binds to the inner ear-speciﬁc short-chain collagen otolin-1, and the resultingcomplex forms ring-like...

Xem thêm